By definition, eukaryotic cells are made up of different compartments, each with its own specific functions. This specificity is the result of differences in the protein and lipid compositions of these compartments. Lipids not only act as a physical barrier between compartments, they also play a key role in numerous mechanisms. In particular, lipid...
Published in Bio-protocol
Intracellular bacterial pathogens have evolved to be adept at manipulating host cellular function for the benefit of the pathogen, often by means of secreted virulence factors that target host pathways for modulation. The lysosomal pathway is an essential cellular response pathway to intracellular pathogens and, as such, represents a common target ...
Published in BMC Cancer
Background Glioblastoma (GBM) is an aggressive brain tumor that exhibits resistance to current treatment, making the identification of novel therapeutic targets essential. In this context, cellular prion protein (PrPC) stands out as a potential candidate for new therapies. Encoded by the PRNP gene, PrPC can present increased expression levels in GB...
Ces dernières années, l'intérêt porté à la sécrétion de vésicules membranaires, appelées vésicules extracellulaires (VE), n'a fait que grandir. Les VE, qui sont sécrétées par toutes les cellules de l'organisme, sont devenues des acteurs majeurs de la communication intercellulaire. Elles peuvent agir localement au sein d'un tissu ou à plus longue di...
Published in Acta pharmaceutica Sinica. B
Protein corona (PC) has been identified to impede the transportation of intravenously injected nanoparticles (NPs) from blood circulation to their targeted sites. However, how intestinal PC (IPC) affects the delivery of orally administered NPs are still needed to be elucidated. Here, we found that IPC exerted "positive effect" or "negative effect" ...
Published in Cellular and Molecular Life Sciences: CMLS
Mitochondria are present in the pre- and post-synaptic regions, providing the energy required for the activity of these very specialized neuronal compartments. Biogenesis of synaptic mitochondria takes place in the cell body, and these organelles are then transported to the synapse by motor proteins that carry their cargo along microtubule tracks. ...
Published in Fluids and barriers of the CNS
The detailed mechanisms by which the transferrin receptor (TfR) and associated ligands traffic across brain capillary endothelial cells (BECs) of the CNS-protective blood-brain barrier constitute an important knowledge gap within maintenance and regulation of brain iron homeostasis. This knowledge gap also presents a major obstacle in research aimi...
Published in European journal of pharmacology
The most prevalent cystic fibrosis (CF)-causing mutation - F508del - impairs the folding of CFTR protein, resulting in its defective trafficking and premature degradation. Small molecules termed correctors may rescue F508del-CFTR and therefore constitute promising pharmacotherapies acting on the fundamental cause of the disease. Here, we screened a...
Published in Methods in molecular biology (Clifton, N.J.)
The Retention Using Selective Hooks (RUSH) system allows for the synchronized release of one or more proteins of interest from a donor endomembrane compartment, usually the endoplasmic reticulum, and the subsequent monitoring of their traffic toward acceptor compartments. Here we describe the RUSH system applied to cytotoxic T cells to characterize...
Published in Methods in molecular biology (Clifton, N.J.)
Endocannabinoids (eCBs) are endogenous lipids able to bind to cannabinoid receptors, the primary molecular targets of the cannabis (Cannabis sativa) active principle Δ9-tetrahydrocannabinol. During the last 20 years, several N-acylethanolamines and acylesters have been shown to act as eCBs, and a complex array of receptors, metabolic enzymes, and t...
