Álvarez-Palomo, Belén García-Martinez, Iris Gayoso, Jorge Raya, Angel Veiga, Anna Abad, María Luisa Eiras, Adolfo Guzmán-Fulgencio, María Luis-Hidalgo, Mar Eguizabal, Cristina
...
Published in
Stem Cell Research & Therapy
BackgroundiPSC (induced pluripotent stem cells) banks of iPSC lines with homozygous HLA (human leukocyte antigen) haplotypes (haplobanks) are proposed as an affordable and off-the-shelf approach to allogeneic transplantation of iPSC derived cell therapies. Cord blood banks offer an extensive source of HLA-typed cells suitable for reprogramming to i...
Rawat, Nidhi Singh, Manoj Kumar Sharma, Tushar Vats, Preeti Nagoorvali, D Palta, Prabhat Chauhan, Manmohan Singh Manik, Radhey Sham
Published in
Animal biotechnology
Many contrasting reports are available on generation of bovine induced pluripotent stem cells (iPSCs) employing different timelines and culture conditions which signifies reprogramming process varies between species and cell types. The present study determines an optimum time period required to re-initiate reprogramming events in buffalo fibroblast...
Pain, Bertrand
Published in
Veterinary Research
Organoids are three-dimensional structures that are derived from the self-organization of stem cells as they differentiate in vitro. The plasticity of stem cells is one of the major criteria for generating organoids most similar to the tissue structures they intend to mimic. Stem cells are cells with unique properties of self-renewal and differenti...
Wray, Selina
Published in
Seminars in cell & developmental biology
Neurodegenerative Diseases such as Alzheimer's Disease represent a major public health challenge, with no disease modifying therapies available. The availability of induced pluripotent stem cells from patients with phenotypes and genotypes of interest, that can be subsequently differentiated in vitro into disease-affected cell types, has revolution...
Xiang, Yangfei Cakir, Bilal Park, In-Hyun
Published in
Seminars in cell & developmental biology
Brain organoids, three-dimensional neural cultures recapitulating the spatiotemporal organization and function of the brain in a dish, offer unique opportunities for investigating the human brain development and diseases. To model distinct parts of the brain, various region-specific human brain organoids have been developed. In this article, we rev...
Duong, Angela Evstratova, Alesya Sivitilli, Adam Hernandez, J. Javier Gosio, Jessica Wahedi, Azizia Sondheimer, Neal Wrana, Jeff L. Beaulieu, Jean-Martin Attisano, Liliana
...
Published in
Scientific Reports
Mitochondrial health plays a crucial role in human brain development and diseases. However, the evaluation of mitochondrial health in the brain is not incorporated into clinical practice due to ethical and logistical concerns. As a result, the development of targeted mitochondrial therapeutics remains a significant challenge due to the lack of appr...
Lee, Yee-Ki Sala, Luca Mura, Manuela Rocchetti, Marcella Pedrazzini, Matteo Ran, Xinru Mak, Timothy S H Crotti, Lia Sham, Pak C Torre, Eleonora
...
Published in
Cardiovascular research
In long QT syndrome (LQTS) patients, modifier genes modulate the arrhythmic risk associated with a disease-causing mutation. Their recognition can improve risk stratification and clinical management, but their discovery represents a challenge. We tested whether a cellular-driven approach could help to identify new modifier genes and especially thei...
Maroli, Giovanni Braun, Thomas
Published in
Cardiovascular research
Knowledge about the molecular mechanisms regulating cardiomyocyte (CM) proliferation and differentiation has increased exponentially in recent years. Such insights together with the availability of more efficient protocols for generation of CMs from induced pluripotent stem cells (iPSCs) have raised expectations for new therapeutic strategies to tr...
Navarro-Guerrero, Elena Tay, Chwen Whalley, Justin P. Cowley, Sally A. Davies, Ben Knight, Julian C. Ebner, Daniel
Published in
Scientific Reports
Genome engineering using CRISPR/Cas9 technology enables simple, efficient and precise genomic modifications in human cells. Conventional immortalized cell lines can be easily edited or screened using genome-wide libraries with lentiviral transduction. However, cell types derived from the differentiation of induced Pluripotent Stem Cells (iPSC), whi...
Häkli, Martta Kreutzer, Joose Mäki, Antti-Juhana Välimäki, Hannu Lappi, Henna Huhtala, Heini Kallio, Pasi Aalto-Setälä, Katriina Pekkanen-Mattila, Mari
Published in
Scientific Reports
Ischemic heart disease is a major cause of death worldwide, and the only available therapy to salvage the tissue is reperfusion, which can initially cause further damage. Many therapeutics that have been promising in animal models have failed in human trials. Thus, functional human based cardiac ischemia models are required. In this study, a human ...
