Blanco, Elena Perez-Andres, Martin Sanoja-Flores, Luzalba Wentink, Marjolein Pelak, Ondrej Martín-Ayuso, Marta Grigore, Georgiana Torres-Canizales, Juan López-Granados, Eduardo Kalina, Tomas
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Published in
Journal of immunological methods
The clinical value of assessing immunoglobulin (Ig)G and IgA subclasses in addition to the isotypes of soluble Igs in serum has been well established. >20years ago, the International Union of Immunological Societies and the World Health Organization performed collaborative studies in order to validate antibody (Ab) clones for the detection of IgG a...
Kooij, Gijs Derada Troletti, Claudio Leuti, Alessandro Norris, Paul C Riley, Ian Albanese, Maria Ruggieri, Serena Libreros, Stephania van der Pol, Susanne M A van Het Hof, Bert
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Published in
Haematologica
Chronic inflammation is a key pathological hallmark of multiple sclerosis and suggests that resolution of inflammation, orchestrated by specialized pro-resolving lipid mediators, is impaired. Here, through targeted-metabololipidomics in peripheral blood of patients with multiple sclerosis, we revealed that each disease form was associated with dist...
Mei, Jiang Na, Li Dexiang, Ji Fei, Li Zhanglin, Zhang
Published in
Turkish journal of haematology : official journal of Turkish Society of Haematology
Yuan, Bo Smith, Janice Ewton, April Pingali, Sai Ravi Zieske, Arthur Breman, Amy
Van Nguyen, Thao Alfaro, Andrea C
Published in
Fish & shellfish immunology
Flow cytometry (FCM) is routinely used in fundamental and applied research, clinical practice, and clinical trials. In the last three decades, this technique has also become a routine tool used in immunological studies of molluscs to analyse physical and chemical characteristics of haemocytes. Here, we briefly review the current implementation of F...
Arbore, Giuseppina Henley, Tom Biggins, Laura Andrews, Simon Richard Vigorito, Elena Turner, Martin Leyland, Rebecca
A fast antibody response can be critical to contain rapidly dividing pathogens. This can be achieved by the expansion of antigen-specific B-cells in response to T-cell help followed by differentiation into plasmablasts. MicroRNA-155 (miR-155) is required for optimal T-cell dependent extrafollicular responses via regulation of PU.1, though the cellu...
Páral, Petr Báječný, Martin Savvulidi, Filipp Nečas, Emanuel
Published in
Methods in molecular biology (Clifton, N.J.)
The thymidine analogues BrdU (5-bromo-2´-deoxyuridine) and EdU (5-ethynyl-2´-deoxyuridine) are routinely used for determination of the cells synthesizing DNA in the S-phase of the cell cycle. Availability of the anti-BrdU antibody clone MoBu-1 detecting only BrdU allowed to develop a method for the sequential DNA labelling by these two thymidine an...
Rothammer, Anna Sage, Eva K. Werner, Caroline Combs, Stephanie E. Multhoff, Gabriele
Published in
Radiation Oncology
BackgroundBreast cancer is the most common invasive tumor in women worldwide and the second cause of cancer-related deaths. After breast conserving surgery the tumor bed gets irradiated. Radiation-induced tumor cell death has been found to be associated with the release of damage-associated molecular patterns (DAMPs) including free Hsp70 that can s...
Garcia-Prat, Marina Álvarez-Sierra, Daniel Aguiló-Cucurull, Aina Salgado-Perandrés, Sandra Briongos-Sebastian, Sara Franco-Jarava, Clara Martin-Nalda, Andrea Colobran, Roger Montserrat, Isabel Hernández-González, Manuel
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Published in
Cytometry. Part B, Clinical cytometry
For the accurate diagnosis of immunodeficiencies is crucial to compare patients' immunology laboratory values with age-sex matched controls, yet there is a paucity of normal values for most populations. To define appropriate reference values of extended lymphocyte subpopulations and T-cell receptor excision circle (TRECs) levels in healthy pediatri...
Raskovalova, Tatiana Berger, Marc Jacob, Marie-Christine Park, Sophie Campos, Lydia Aanei, Carmen Mariana Kasprzak, Julie Pereira, Bruno Labarere, Jose Cesbron, Jean-Yves
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Suspicion of myelodysplastic syndromes is one of the commonest reasons for bone marrow aspirate in elderly patients presenting with persistent peripheral blood cytopenia of unclear etiology. A peripheral blood assay that accurately rules out myelodysplastic syndromes would have major benefits. The diagnostic accuracy of the intraindividual robust c...
