Abscisic Acid Uridine Diphosphate Glucosyltransferases Play a Crucial Role in Abscisic Acid Homeostasis in Arabidopsis
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A novel xylan degrading beta-D-xylosidase: purification and biochemical characterization
SISBIOTA CNPq
A novel xylan degrading beta-D-xylosidase: purification and biochemical characterization
SISBIOTA CNPq
A novel xylan degrading beta-D-xylosidase: purification and biochemical characterization
SISBIOTA CNPq
Biochemical characterization of a maize stover beta-exoglucanase and its use in lignocellulose conversion
Plant is one of the important resources for glycosyl hydrolase production. A beta-exoglucanase with molecular weight of 63.1 kDa was purified from fresh maize stover and subjected to enzymatic characterization. The optimal temperature and pH of the beta-exoglucanase was 40 degrees C and 6.0, respectively. The beta-exoglucanase was active against p-...
The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a beta-gl...
The relative contributions to the specificity and catalysis of aglycone, of residues E190, E194, K201 and M453 that form the aglycone-binding site of a beta-glycosidase from Spodoptera frugiperda (EC 3.2.1.21), were investigated through site-directed mutagenesis and enzyme kinetic experiments. The results showed that E190 favors the binding of the ...
The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a beta-gl...
The relative contributions to the specificity and catalysis of aglycone, of residues E190, E194, K201 and M453 that form the aglycone-binding site of a beta-glycosidase from Spodoptera frugiperda (EC 3.2.1.21), were investigated through site-directed mutagenesis and enzyme kinetic experiments. The results showed that E190 favors the binding of the ...
The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a beta-gl...
The relative contributions to the specificity and catalysis of aglycone, of residues E190, E194, K201 and M453 that form the aglycone-binding site of a beta-glycosidase from Spodoptera frugiperda (EC 3.2.1.21), were investigated through site-directed mutagenesis and enzyme kinetic experiments. The results showed that E190 favors the binding of the ...
A novel thermostable alpha-galactosidase from the thermophilic fungus Thermomyees lanuginosus CBS 395.62/b: Purification...
Converting a beta-glycosidase into a beta-transglycosidase by directed evolution
Directed evolution was applied to the beta-glycosidase of Thermus thermophilus in order to increase its ability to synthesize oligosaccharide by transglycosylation. Wild-type enzyme was able to transfer the glycosyl residue with a yield of 50% by self-condensation and of about 8% by transglycosylation on disaccharides without nitrophenyl at their r...
