Vlacil, Ann-Kathrin Schuett, Jutta Ruppert, Volker Soufi, Muhidien Oberoi, Raghav Shahin, Kinan Wächter, Christian Tschernig, Thomas Lei, Yu Liu, Fan
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Published in
Basic Research in Cardiology
Atherosclerosis is crucially fueled by inflammatory pathways including pattern recognition receptor (PRR)-related signaling of the innate immune system. Currently, the impact of the cytoplasmic PRRs nucleotide-binding oligomerization domain-containing protein (NOD) 1 and 2 is incompletely characterized. We, therefore, generated Nod1/Nod2 double kno...
Drews, Barbara Landaverde, Luis Flores Kühl, Anja Drews, Ulrich
Published in
BMC Developmental Biology
BackgroundIn normal mammalian development a high percentage of implantations is lost by spontaneous resorption. This is a major problem in assisted reproduction and blastocyst transfer. Which embryo will be resorbed is unpredictable. Resorption is very fast, so that with conventional methods only final haemorrhagic stages are encountered.Here we de...
Wen, Yichuan Ahmad, Feroz Mohri, Zahra Weinberg, Peter D Leake, David S
Published in
Atherosclerosis
We have shown previously that low density lipoprotein (LDL) aggregated by vortexing is internalised by macrophages and oxidised by iron in lysosomes to form the advanced lipid/protein oxidation product ceroid. We have now used sphingomyelinase-aggregated LDL, a more pathophysiological form of aggregated LDL, to study lysosomal oxidation of LDL and ...
Bhansali, Shipra Khatri, Shivani Dhawan, Veena
Published in
Lipids in Health and Disease
BackgroundIncreased macrophage and foam cell apoptosis during early atherogenesis retards plaque progression by impeding foam cell formation, suppressing inflammation and limiting lesion cellularity. Our previous in vitro study in THP1 macrophages demonstrated that Terminalia Arjuna (TA) attenuates dual-specificity phosphatase1 (DUSP1), a key negat...
Liu, Cong-Lin Zhang, Xian Liu, Jing Wang, Yunzhe Sukhova, Galina K. Wojtkiewicz, Gregory R. Liu, Tianxiao Tang, Rui Achilefu, Samuel Nahrendorf, Matthias
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Published in
Nature Communications
Na+-H+ exchanger 1 (Nhe1) regulates extracellular pH by extruding protons in exchange for extracellular Na+ . Here, Liu et al. show that Nhe1 promotes the development and acidification of atherosclerotic lesions and that pH-sensitive probes can be used to monitor plaque growth and acidification.
Gallagher, Hayley Williams, Jessica O Ferekidis, Nele Ismail, Alaa Chan, Yee-Hung Michael, Daryn R Guschina, Irina A Tyrrell, Victoria J O'Donnell, Valerie B Harwood, John L
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Published in
Biochimica et biophysica acta. Molecular basis of disease
Atherosclerosis and its complications are responsible for one in three global deaths. Nutraceuticals show promise in the prevention and treatment of atherosclerosis but require an indepth understanding of the mechanisms underlying their actions. A previous study showed that the omega-6 fatty acid, dihomo-γ-linolenic acid (DGLA), attenuated atherosc...
Wang, Jing Yannie, Paul J Ghosh, Siddhartha S Ghosh, Shobha
Published in
FEBS letters
A causal relationship exists between macrophage cholesterol levels and inflammation, for example, Interleukin-1β (IL-1β) secretion. A decrease in intracellular K+ is essential for inflammasome activation/IL-1β secretion and, herein, we examined the hypothesis that cellular cholesterol affects K+ -channel activity and K+ -efflux using mouse peritone...
Ouimet, Mireille Barrett, Tessa J. Fisher, Edward A.
Published in
Circulation research
Cardiovascular disease (CVD), with atherosclerosis as the major underlying factor, remains the leading cause of death worldwide. It is well established that cholesterol ester-enriched foam cells are the hallmark of atherosclerotic plaques. Multiple lines of evidence support that enhancing foam cell cholesterol efflux by high density lipoprotein (HD...
Mehrpouri, Mahdieh Bashash, Davood Mohammadi, Mohammad Hossien Gheydari, Mohammad Esmail Satlsar, Esmail Shahabi Hamidpour, Mohsen
Published in
Turkish Journal of Hematology
Objective: Far beyond hemostasis and thrombosis, significant evidence has indicated the critical role of platelets in atherosclerosis. SDF-1 is among the pro-inflammatory chemokines that are increased in platelets of patients with coronary artery disease (CAD). The goal of the current work is to identify the in vitro effect of platelets from either...
Yamazaki, Hisataka Takahashi, Manabu Wakabayashi, Tetsuji Sakai, Kent Yamamuro, Daisuke Takei, Akihito Takei, Shoko Nagashima, Shuichi Yagyu, Hiroaki Sekiya, Motohiro
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Published in
Journal of atherosclerosis and thrombosis
Acyl-CoA cholesterol acyltransferase 1 (ACAT1) esterifies free cholesterol to cholesteryl esters (CE), which are subsequently hydrolyzed by neutral cholesterol ester hydrolase 1 (NCEH1). The elimination of ACAT1 in vitro reduces the amounts of CE accumulated in Nceh1-deficient macrophages. The present study aimed at examining whether the loss of AC...
