Background In this study, we investigated the effect of the mechanical loading history on the expression of receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) in MLO-Y4 osteocyte-like cells. Methods Three hours after MLO-Y4 osteocytes were seeded, a continuous compressive force (CCF) of 31 dynes/cm2 with or withou...
Published in Data in Brief
For a long time, traditional purification and extraction methods for the native Torpedo californica nicotinic acetylcholine receptor in lipid-like detergent complex (nAChR-DC) have compromised its purity, functionality and X-ray structural studies possibility. The dataset presented in this article provide a characterization of the Torpedo californi...
Published in Bio-protocol
Liquid-liquid phase separation (LLPS) underlies the physiological assembly of many membrane-less organelles throughout the cell. However, dysregulation of LLPS may mediate the formation of pathological aggregates associated with neurodegenerative diseases. Here, we present complementary experimental approaches to study protein aggregation within an...
Published in Methods in cell biology
The exocytosis process delivers proteins, lipids, and carbohydrates to the plasma membrane or the extracellular space to sustain plant cell growth, development, and response to environmental stimuli. Plant exocytosis is highly dynamic and requires the coordinated functions of multiple cellular components such as tethering complexes, GTPase signalin...
Published in Methods in molecular biology (Clifton, N.J.)
Exocytosis is a fundamental process essential for many cellular functions by targeting signal peptides, proteins, and cell wall components to the plasma membrane (PM) or extracellular matrix. Conventional methods, such as FRAP, often underestimate the exocytosis rate of a specific molecule, because retrieval of the molecules from the PM by endocyto...
Published in Colloids and surfaces. B, Biointerfaces
Gangliosides are glycolipids that are enriched on the outer surface of cell membranes. Gangliosides are receptors for a number of signaling molecules and toxins, and therefore are often incorporated into biosensors. Many of these biosensors incorporate gangliosides into supported lipid bilayers which are formed by the spontaneous rupture of unilame...
Published in Small (Weinheim an der Bergstrasse, Germany)
Experimental setups to produce and to monitor model membranes have been successfully used for decades and brought invaluable insights into many areas of biology. However, they all have limitations that prevent the full in vitro mimicking and monitoring of most biological processes. Here, a suspended physiological bilayer-forming chip is designed fr...
Published in Biochimica et biophysica acta. Molecular cell research
Doublecortin X (DCX) plays essential roles in neuronal development via its regulation of cytoskeleton dynamics. This is mediated through direct interactions between its doublecortin (DC) domains (DC1 and DC2) with microtubules (MTs) and indirect association with actin filaments (F-ACT). While the regulatory role of the DCX C-terminus following DC2 ...
Published in Journal of cell science
Transcription factor mobility is a determining factor in the regulation of gene expression. Here, we have studied the intranuclear dynamics of the glucocorticoid receptor (GR) by using fluorescence recovery after photobleaching and single-molecule microscopy. First, we have described the dynamic states in which the GR occurs. Second, we have analyz...
Published in PLoS biology
Tip growth has been studied in pollen tubes, root hairs, and fungal and oomycete hyphae and is the most widely distributed unidirectional growth process on the planet. It ensures spatial colonization, nutrient predation, fertilization, and symbiosis with growth speeds of up to 800 μm h-1. Although turgor-driven growth is intuitively conceivable, a ...
