Liu, Xiaolin Lertsethtakarn, Paphavee Mariscal, Vanessa Yildiz, Fitnat Ottemann, Karen
UNLABELLED: Motility promotes biofilm initiation during the early steps of this process: microbial surface association and attachment. Motility is controlled in part by chemotaxis signaling, so it seems reasonable that chemotaxis may also affect biofilm formation. There is a gap, however, in our understanding of the interactions between chemotaxis ...
Link, Kathryn G Guy, Robert D Thomases, Becca Arratia, Paulo E
Many microorganisms propel themselves through complex media by deforming their flagella. The beat is thought to emerge from interactions between forces of the surrounding fluid, the passive elastic response from deformations of the flagellum and active forces from internal molecular motors. The beat varies in response to changes in the fluid rheolo...
Bryant, Owain J Fraser, Gillian M
UNLABELLED: Type III secretion systems (T3SSs) are essential for motility and virulence in many bacterial pathogens. Proteins destined for the flagellar T3SS contain at least two export signals in their N-terminal D0 domain. Here, we describe a third carboxy (C)-terminal signal in early flagellar subunits that facilitates subunit targeting to the e...
Liu, XF Onda, M Schlomer, J Bassel, L Kozlov, S Tai, C-H Zhou, Q Liu, W Tsao, H-E Hassan, R
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Bacterial flagella and type IV pili (TFP) are surface appendages that enable motility and mechanosensing through distinct mechanisms. These structures were previously thought to have no components in common. Here, we report that TFP and some flagella share proteins PilO, PilN, and PilM, which we identified as part of the Helicobacter pylori flagell...
Chen, Mingfei Trotter, Valentine V Walian, Peter J Chen, Yan Lopez, Romario Lui, Lauren M Nielsen, Torben N Malana, Ria Gracielle Thorgersen, Michael P Hendrickson, Andrew J
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Biofilms aid bacterial adhesion to surfaces via direct and indirect mechanisms, and formation of biofilms is considered as an important strategy for adaptation and survival in suboptimal environmental conditions. However, the molecular underpinnings of biofilm formation in subsurface sediment/groundwater ecosystems where microorganisms often experi...
Dupuis, Sophie Girault, Marie-Sophie Le Beulze, Morgane Ialy-Radio, Côme Bermúdez-Guzmán, Luis Ziyyat, Ahmed Barbaux, Sandrine
Acknowledgements: The authors would like to thank A. Schmitt from the Electron Microscopy Facility (Institut Cochin, Paris), R. Onifarasoaniaina from the HISTIM core facility (Institut Cochin, Paris), J. Lesieur and T. Guilbert from the IMAG’IC core facility (Institut Cochin, Paris). The authors thank P. Lorès, A.Touré and M. Whitfield for providin...
Uesaka, Kazuma Inaba, Keita Nishioka, Noriko Kojima, Seiji Homma, Michio Ihara, Kunio
Published in
PeerJ
The motility of Vibrio species plays a pivotal role in their survival and adaptation to diverse environments and is intricately associated with pathogenicity in both humans and aquatic animals. Numerous mutant strains of Vibrio alginolyticus have been generated using UV or EMS mutagenesis to probe flagellar motility using molecular genetic approach...
Shiozaki, Momoko Haas, Kelsey Skinner, Will Zhao, Shumei Guo, Caiying Polacco, Benjamin Yu, Zhiheng Krogan, Nevan Lishko, Polina Kaake, Robyn
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To understand the molecular mechanisms of cellular pathways, contemporary workflows typically require multiple techniques to identify proteins, track their localization, and determine their structures in vitro. Here, we combined cellular cryoelectron tomography (cryo-ET) and AlphaFold2 modeling to address these questions and understand how mammalia...
He, Lei Qin, Jianmei Guo, Jia Wang, Shuai Rong, Haifeng Tong, Meiping
Published in
The Science of the total environment
The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents remain unclear. The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 ...
Klena, Nikolai Maltinti, Giovanni Batman, Umut Pigino, Gaia Guichard, Paul Hamel, Virginie
Published in
Bio-protocol
Expansion microscopy is an innovative method that enables super-resolution imaging of biological materials using a simple confocal microscope. The principle of this method relies on the physical isotropic expansion of a biological specimen cross-linked to a swellable polymer, stained with antibodies, and imaged. Since its first development, several...