ABSTRACT Purpose: Collagen deposition and myofibroblast differentiation are critical factors related to excessive scarring in ocular surgeries. This study evaluated the anti-fibrotic activity of rosmarinic acid on rabbit Tenon’s capsule fibroblasts stimulated with transforming growth factor- β2. Methods: Primary cultures of rabbit Tenon’s capsule f...
Published in BMC Medical Genetics
BackgroundIntermediate filament proteins that construct the nuclear lamina of a cell include the Lamin A/C proteins encoded by the LMNA gene, and are implicated in fundamental processes such as nuclear structure, gene expression, and signal transduction. LMNA mutations predominantly affect mesoderm-derived cell lineages in diseases collectively ter...
Published in Clinical Epigenetics
BackgroundDNA methylation (DNAm) age constitutes a powerful tool to assess the molecular age and overall health status of biological samples. Recently, it has been shown that tissue-specific DNAm age predictors may present superior performance compared to the pan- or multi-tissue counterparts. The skin is the largest organ in the body and bears imp...
Published in Applied biochemistry and biotechnology
Cancer-associated fibroblasts (CAFs) are important ingredient in tumor microenvironment. The dynamic interplay between CAFs and cancer cells plays essential roles during tumor development and progression. However, the mechanisms of intercellular communication between CAFs and cancer cells remain largely unknown. We characterized exosomes secreted f...
Published in BMC Oral Health
BackgroundThree-dimensional (3D) printing is widely used in the fabrication of dental prostheses; however, the influence of dental materials used for 3D printing on temporary restoration of fibroblasts in tissues is unclear. Thus, the influence of different dental materials on fibroblasts were investigated.MethodsDigital light processing (DLP) type...
Published in Aesthetic plastic surgery
New regenerative treatments have emerged with the use of multipotent mesenchymal cells, with special interest in adipose-derived stem cells (ADSCs). In recent years, studies that have sought to identify possible quantitative or qualitative differences in ADSCs derived from different donor subcutaneous adipose tissue have shown divergent results mak...
Published in Respiratory Research
BackgroundIdiopathic pulmonary fibrosis (IPF) is a rapidly progressing disease with challenging management. To find novel effective therapies, better preclinical models are needed for the screening of anti-fibrotic compounds. Activated fibroblasts drive fibrogenesis and are the main cells responsible for the accumulation of extracellular matrix (EC...
Published in Stem Cell Research & Therapy
BackgroundFibroblasts are crucial for supporting normal wound healing. However, the functional state of these cells is impaired in diabetics because of a high-glucose (HG) microenvironment. Small extracellular vesicles (sEVs) have emerged as a promising tool for skin wound treatment. The aim of this study was to investigate the effects of sEVs deri...
Fig 3a) LDH attachment assay. Fig 4) QNM, KPFM and PFM data taken using Bruker Multimode 8 atomic force microscope with a MESP-RC-V2 probe (Bruker). Fig S7) QNM data. Fig S9) KPFM data. Fig S10) LDH attachment assay. Fig S11) QNM data. Fig S12) KPFM data. Fig S13) PFM data. Fig S14) QNM data. Fig S15b) XRD data. Fig S15c) LDH attachment assay. See ...
Published in BMC Molecular and Cell Biology
BackgroundGene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than immortalized cells, and have a limited proliferative capacity. Improving the gene transfer by using ...
