Falak, Samreen Macdonald, Rainer Busby, Eloise J O'Sullivan, Denise M Milavec, Mojca Plauth, Annabell Kammel, Martin Zeichhardt, Heinz Grunert, Hans-Peter Kummrow, Andreas
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Published in
Methods (San Diego, Calif.)
Viral load monitoring in human immunodeficiency virus type 1 (HIV-1) infection is often performed using reverse transcription quantitative PCR (RT-qPCR) to observe response to treatment and identify the development of resistance. Traceability is achieved using a calibration hierarchy traceable to the International Unit (IU). IU values are determine...
Milavec, Mojca Pavšič, Jernej Bogožalec Košir, Alexandra Jones, Gerwyn M O'Sullivan, Denise M Devonshire, Alison S Van Heuverswyn, Fran Karczmarczyk, Maria Neeb, Jannika Plauth, Annabell
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Published in
Methods (San Diego, Calif.)
A candidate digital PCR (dPCR)-based reference measurement procedure for quantification of human cytomegalovirus (hCMV) was evaluated in 10 viral load comparison schemes (seven external quality assessment (EQA) and three additional training schemes) organized by INSTAND e.V. over four years (between September 2014 and March 2018). Four metrology in...
Manjengwa, Julius Zakaryan, Arsen Orelle, Arnaud Fensham, Vivian Razafindranovonar, Arihanta Aleksanyan, Naira Kotsinyan, Nune Danielyan, Hrant Tumanyan, Pertsh Davtyan, Zaruhi
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Published in
One Health
Introduction Early warning and objective evidence of systematic errors in laboratory diagnosis ensures evidence based corrective and preventive actions that instill patient safety and confidence. External quality assessment contributes significantly to the above as an essential component of laboratory quality assurance. However, implementation of E...
Vu, Quang Huy Van, Hy Triet Tran, Van Thanh Huynh, Thi Diem Phuc Nguyen, Van Chinh Le, Dinh Thanh
Published in
Practical laboratory medicine
The external quality assessment (EQA) scheme is particularly important for laboratory performance evaluation. Peripheral blood smears are necessary to identify morphological features, and the procedure for preparing such smears must be robust to meet the ISO 15189 standard. Although blood smear preparation is a routine activity in medical laborator...
Leung, Vivian K Y Deng, Yi-Mo Todd, Angela Peck, Heidi Buettner, Iwona Zakis, Tasoula Subbarao, Kanta Barr, Ian G Nahapetyan, Karen Inbanathan, Francis Y
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Published in
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
Influenza viruses must be amplified in cell culture for detailed antigenic analysis and for phenotypic assays assessing susceptibility to antiviral drugs or for other assays. Following on from the first external quality assessment (EQA) for isolation and identification of influenza viruses using cell culture techniques in 2016, a follow up EQA was ...
Yoon, Young Ahn Lee, Yong-Wha Kim, Sollip Lee, Kyunghoon Park, Hyung-Doo Chun, Sail Min, Won-Ki
Published in
Annals of Laboratory Medicine
Background Total cholesterol concentration measurement is important in the diagnosis of dyslipidemia and evaluation of cardiovascular disease risk factors. Measurement reliability for obtaining an accurate total cholesterol concentration requires procedure standardization. We evaluated the standardization status for total cholesterol concentration ...
Jurmeister, Philipp Vollbrecht, Claudia Jöhrens, Korinna Aust, Daniela Behnke, Anke Stenzinger, Albrecht Penzel, Roland Endris, Volker Schirmacher, Peter Fisseler-Eckhoff, Annette
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Published in
Virchows Archiv
With this external quality assessment (EQA) scheme, we aim to investigate the diagnostic performance of the currently available methods for the detection of ALK alterations in non-small cell lung cancer on a national scale, namely, in situ hybridization (ISH), immunohistochemistry (IHC), and RNA/DNA sequencing (NGS). The EQA scheme cohort consisted...
Keppens, Cleo Dequeker, Elisabeth Mc Pauwels, Patrick Ryska, Ales 't Hart, Nils von der Thüsen, Jan H
Published in
Virchows Archiv : an international journal of pathology
Programmed death ligand 1 (PD-L1) immunohistochemistry (IHC) is accepted as a predictive biomarker for the selection of immune checkpoint inhibitors. We evaluated the staining quality and estimation of the tumor proportion score (TPS) in non-small-cell lung cancer during two external quality assessment (EQA) schemes by the European Society of Patho...
Keppens, Cleo Schuuring, Ed Dequeker, Elisabeth M C
Published in
Virchows Archiv : an international journal of pathology
External quality assessment (EQA) schemes assess the performance of predictive biomarker testing in lung and colorectal cancer and have previously demonstrated variable error rates. No information is currently available on the underlying causes of incorrect EQA results in the laboratories. Participants in EQA schemes by the European Society of Path...
Keppens, Cleo Boone, Elke Gameiro, Paula Tack, Véronique Moreau, Elisabeth Hodges, Elizabeth Evans, Paul Brüggemann, Monika Carter, Ian Lenze, Dido
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Published in
Virchows Archiv
Clonality analysis of immunoglobulin (IG) or T-cell receptor (TR) gene rearrangements is routine practice to assist diagnosis of lymphoid malignancies. Participation in external quality assessment (EQA) aids laboratories in identifying systematic shortcomings. The aim of this study was to evaluate laboratories’ improvement in IG/TR analysis and int...