Nakkarach, Atchareeya Foo, Hooi Ling Song, Adelene Ai-Lian Mutalib, Nur Elina Abdul Nitisinprasert, Sunee Withayagiat, Ulaiwan
Published in
Microbial Cell Factories
BackgroundExtracellular metabolites of short chain fatty acids (SCFA) excreted by gut microbiota have been reported to play an important role in the regulation of intestinal homeostasis. Apart from supplying energy, SCFA also elicit immune stimulation in animal and human cells. Therefore, an attempt was conducted to isolate SCFA producing bacteria ...
Park, Jin Yeong Lim, Jeong-Hyeon Ahn, Joong-Hoon Kim, Bong-Gyu
Published in
Applied Biological Chemistry
Resveratrol (3,5,4′-trihydroxy-trans-stilbene) is a phenolic compound widely used in pharmaceutics and nutraceutics. Although resveratrol is produced by some plant species, including grapes, peanuts, and berries, the content of resveratrol and its derivatives are very low. Therefore, an alternative biosynthetic method using microorganisms, such as ...
Chen, Jie Yang, Hao Feng, Yanru Shi, Qiuxiao Li, Zhao Tao, Ze Fan, Jie Jin, Youmei Li, Shengfu Cheng, Jingqiu
...
Published in
Applied Microbiology and Biotechnology
Abstract Due to their potent immune stimulation, tumor necrosis factor alpha (TNFα) variants with tumor-homing activity are attractive as novel antitumor drugs. The promising antitumor effect of NGR-TNFα in clinical trials triggered extensive interest in developing novel tumor-homing TNFα variants in recent years. Owing to its promising antitumor e...
Nicchi, Sonia Giuliani, Maria Giusti, Fabiola Pancotto, Laura Maione, Domenico Delany, Isabel Galeotti, Cesira L. Brettoni, Cecilia
Published in
Microbial Cell Factories
BackgroundThe display of recombinant proteins on cell surfaces has a plethora of applications including vaccine development, screening of peptide libraries, whole-cell biocatalysts and biosensor development for diagnostic, industrial or environmental purposes. In the last decades, a wide variety of surface display systems have been developed for th...
Alonso Villela, Susana María Ghezal-Kraïem, Hazar Bouhaouala-Zahar, Balkiss Bideaux, Carine Aceves Lara, César Arturo Fillaudeau, Luc
In the pharmaceutical industry, nanobodies show promising properties for its application in serotherapy targeting the highly diffusible scorpion toxins. The production of recombinant nanobodies in Escherichia coli has been widely studied in shake flask cultures in rich medium. However, there are no upstream bioprocess studies of nanobody production...
Gouyau, Jimmy Duval, Raphael Boudier, Ariane Lamouroux, Emmanuel
Multidrug-resistant (MDR) bacteria constitute a global health issue. Over the past ten years, interest in nanoparticles, particularly metallic ones, has grown as potential antibacterial candidates. However, as there is no consensus about the procedure to characterize the metallic nanoparticles (MNPs; i.e., metallic aggregates) and evaluate their an...
Stargardt, Patrick Striedner, Gerald Mairhofer, Juergen
Published in
Microbial Cell Factories
BackgroundPrecise regulation of gene expression is of utmost importance for the production of complex membrane proteins (MP), enzymes or other proteins toxic to the host cell. In this article we show that genes under control of a normally Isopropyl β-d-1-thiogalactopyranoside (IPTG)-inducible PT7-lacO promoter can be induced solely with l-arabinose...
Hoogland, Inge C M Westhoff, Dunja Engelen-Lee, Joo-Yeon Valls Seron, Mercedes Houben-Weerts, Judith H M P van Westerloo, David J van der Poll, Tom van Gool, Willem A van de Beek, Diederik
Published in
Cells
Systemic infection is an important risk factor for the development cognitive impairment and neurodegeneration in older people. Animal experiments show that systemic challenges with live bacteria cause a neuro-inflammatory response, but the effect of age on this response in these models is unknown. Young (2 months) and middle-aged mice (13-14 months...
He, Yijie Liu, Yanhong Ji, Peng
Published in
Metabolites
This study explored the metabolomic profiles in ileal mucosa and colon digesta in response to enterotoxigenic Escherichia coli F18 (ETEC) infection and dietary use of probiotics and low-dose antibiotics. Weaned pigs (n = 48, 6.17 ± 0.36 kg body weight) were randomly allotted to one of four treatments. Pigs in the negative control (NC) were fed a ba...
Sy, Brandon M. Tree, Jai J.
Published in
Frontiers in Cellular and Infection Microbiology
Enteric and extraintestinal pathotypes of Escherichia coli utilize a wide range of virulence factors to colonize niches within the human body. During infection, virulence factors such as adhesins, secretions systems, or toxins require precise regulation and coordination to ensure appropriate expression. Additionally, the bacteria navigate rapidly c...