Ce travail de thèse avait deux objectifs principaux. D’une part, la recherche de nouvelles ATA via le criblage de banques d’enzymes issues de la biodiversité et d’autre part le développement de nouveaux procédés biocatalytique associant AL et TA. Nous avons développé deux tests de criblages originaux permettant le criblage haut débit des collection...
Multi-step cascade reactions have gained increasing attention in the biocatalysis field in recent years. In particular, multi-enzymatic cascades can achieve high molecular complexity without workup of reaction intermediates thanks to the enzymes’ intrinsic selectivity; and where enzymes fall short, organo- or metal catalysts can further expand the ...
Published in Applied microbiology and biotechnology
Raspberry ketone is a widely used flavor compound in food and cosmetic industry. Several processes for its biocatalytic production have already been described, but either with the use of genetically modified organisms (GMOs) or incomplete conversion of the variety of precursors that are available in nature. Such natural precursors are rhododendrol ...
Published in Biosensors & bioelectronics
Horseradish peroxidase (HRP)-based electrochemical immunoassays are considered promising techniques for point-of-care clinical diagnostics, but the necessary addition of unstable H2O2 in the enzymatic system may hinder their practical application. Although glucose oxidase (GOx) has been widely explored for in situ generation of H2O2 in HRP-based im...
Published in Colloids and surfaces. B, Biointerfaces
Keratin is widely used in the biomaterial application, but the keratin prepared by the physical or chemical approach has relatively low molecular weight and mechanical properties. Here we report the preparation of high molecular keratin (HMK) with molecular weight of 120 kDa via multi-enzyme cascade pathway and its application in wound healing. Bri...
Published in Chembiochem : a European journal of chemical biology
Bacterial luciferase (Lux) catalyzes a bioluminescence reaction by using long-chain aldehyde, reduced flavin and molecular oxygen as substrates. The reaction can be applied in reporter gene systems for biomolecular detection in both prokaryotic and eukaryotic organisms. Because reduced flavin is unstable under aerobic conditions, another enzyme, fl...
Published in Biotechnology and bioengineering
The efficiency of a versatile in vivo cascade involving a promiscuous alcohol dehydrogenase, obtained from a biodiversity search, and a Baeyer-Villiger monooxygenase was enhanced by the independent control of the production level of each enzyme to produce ε-caprolactone and 3,4-dihydrocoumarin. This goal was achieved by adjusting the copy number pe...
La transcétolase thermostable de Geobacillus stearothermophilus (TKgst, EC 2.2.1.1) permet de synthétiser efficacement à haute température des cétoses à 4, 5 et 6 atomes de carbone de configuration d-thréo (3S, 4R), par formation stéréosélective d’une liaison C-C, à partir d’aldéhydes α-hydroxylés (2R) à courte chaîne. L’objectif de ces travaux est...
L'une des voies principales du métabolisme cellulaire est la voie des Pentoses Phosphates (PPP). Cette voie métabolique est composée de deux cascades enzymatiques, une voie oxydative et une voie non oxydative. La voie oxydative de la PPP produit un cofacteur, le NADPH, qui est responsable du processus de détoxification de la cellule par son activit...
The chemo-enzymatic cascade which combines alcohol oxidase from Hansenula polymorpha (AOXHp) with vanadium chloroperoxidase (VCPO), for the production of biobased nitriles from amino acids was investigated. In the first reaction H2O2 (and acetaldehyde) are generated from ethanol and oxygen by AOXHp. H2O2 is subsequently used in the second reaction ...
