Pericardial edema - fluid accumulation within the pericardium - is a frequently observed malformation in zebrafish embryo-based chemical toxicity screens. We recently discovered that the severity of triphenyl phosphate (TPHP)-induced pericardial edema was dependent on the ionic strength of exposure media. TPHP is an aryl phosphate ester (APE) widel...
Background In vitro embryo production is increasingly used for genetic improvement in cattle but bypasses the oviduct environment and exposes the embryos to oxidative stress with deleterious effects on further development. Here we aimed to examine the effect of oviduct epithelial spheroids (OES) on embryo development and quality in terms of morphol...
Cette intervention visera non seulement à comprendre comment la société française régule-t-elle l’équilibre entre autonomie des femmes et vie anténatale, mais aussi à interroger les discours anti-avortement. Il apparaît que ce qui est en jeu dans les débats autour de l’avortement est bien la difficulté à définir clairement le statut de l’être avant...
Dynamic changes in maternal-zygotic transition (MZT) require complex regulation of zygote formation, maternal transcript decay, embryonic genome activation (EGA), and cell cycle progression. Although these changes are well described, some key regulatory factors are still elusive. Sirtuin-1 (SIRT1), an NAD + -dependent histone deacetylase, is a vers...
Preimplantation genetic testing (PGT) has opened unforeseen ways to prevent the spread of severe genetic diseases. By transferring only embryos that do not carry the targeted disease or are healthy carriers, children-to-be are ensured to never develop symptoms in their lifetime. However, transferring healthy carrier embryos may have long-term conse...
This draft examines the “moral labour” of French professionals involved in preimplantation genetic testing, an ethically sensitive practice aimed at preventing the birth of children with hereditary diseases by transferring only "healthy" embryos. While French law forbids discarding healthy-carrier embryos, it leaves a “grey area” regarding the pref...
Southern Distinct Population Segment (sDPS) green sturgeon spawn solely in one stretch of the Sacramento River in California. Management of this spawning habitat is complicated by cold water temperature requirements for the conservation of winter-run Chinook salmon. This study assessed whether low incubation and rearing temperatures resulted in car...
Xenopus embryos provide a favorable material to dissect the sequential steps that lead to dorsal-ventral (D-V) and anterior-posterior (A-P) cell differentiation. Here, we analyze the signaling pathways involved in this process using loss-of-function and gain-of-function approaches. The initial step was provided by Hwa, a transmembrane protein that ...
Although the effects of genetic and environmental perturbations on multicellular organisms are rarely restricted to single phenotypic layers, our current understanding of how developmental programs react to these challenges remains limited. Here, we have examined the phenotypic consequences of disturbing the bicoid regulatory network in early Droso...
Published in Integrative and comparative biology
How animal body plans evolved and diversified is a major question in evolutionary developmental biology. To address this question, it is important to characterize the exact molecular mechanisms that establish the major embryonic axes which give rise to the adult animal body plan. The anterior-posterior (AP) axis is the first axis to be established ...
