This letter presents a biosensor first developed to perform microwave dielectric spectroscopy (MDS) analysis, which is evaluated for in situ electroporation (EP) at the single- cell level. After demonstrating the possibility to characterize living cancer cells in the radio-frequency (RF) sensor by acquir- ing their dielectric properties, we then ev...
Published in Microbiological research
Marine bacteria play vital roles in symbiosis, biogeochemical cycles and produce novel bioactive compounds and enzymes of interest for the pharmaceutical, biofuel and biotechnology industries. At present, investigations into marine bacterial functions and their products are primarily based on phenotypic observations, -omic type approaches and heter...
Published in Scientific reports
Transposon directed insertion-site sequencing (TraDIS), a variant of transposon insertion sequencing commonly known as Tn-Seq, is a high-throughput assay that defines essential bacterial genes across diverse growth conditions. However, the variability between laboratory environments often requires laborious, time-consuming modifications to its prot...
The understanding of the mechanisms involved in DNA electrotransfer in human skin remains modest and limits the clinical development of various biomedical applications, such as DNA vaccination. To elucidate some mechanisms of DNA transfer in the skin following electroporation, we created a model of the dermis using a tissue engineering approach. Th...
Published in Bioelectrochemistry (Amsterdam, Netherlands)
Despite extensive research in recent decades, pancreatic cancer continues to be among the most lethal forms of cancer, with no substantial increase in survival rates. Local recurrences account for approximately 30 per cent of all disease recurrences. With the intent to improve survival, we designed a novel, hybrid treatment strategy consisting of s...
Published in Annals of biomedical engineering
The mechanisms of cell death due to electroporation are still not well understood. Recent studies suggest that cell death due to electroporation is not an immediate all-or-nothing response but rather a dynamic process that occurs over a prolonged period of time. To investigate whether the dynamics of cell death depends on the pulse parameters or ce...
Published in Annals of biomedical engineering
Escherichia coli bacterium is a rod-shaped organism composed of a complex double membrane structure. Knowledge of electric field driven ion transport through both membranes and the evolution of their induced permeabilization has important applications in biomedical engineering, delivery of genes and antibacterial agents. However, few studies have b...
Published in Annals of biomedical engineering
In this paper, a new application of Piezoelectric Transformer (PT)-based power converters to generate high-voltage (HV) bipolar pulses for medical electroporation therapy is proposed. In particular, PT-based power conversion is investigated as an alternative to magnetics-based approaches of generating HV from a relatively low-voltage (LV) input sou...
Published in Annals of biomedical engineering
Pulsed electric field (PEF) technologies treat many types of tissue. Many systems mandate synchronization to the cardiac cycle to avoid the induction of cardiac arrhythmias. Significant differences between PEF systems make the assessment of cardiac safety from one technology to another challenging. A growing body of evidence suggests that shorter d...
Les effets d’impulsions de courtes durées et de très hautes intensités sur les cellules vivantes sont de plus en plus étudiés. En effet, ce type d’exposition a montré une très grande efficacité dans plusieurs domaines de la vie quotidienne tels que les science environnementales, l’agroalimentaire et le biomédicale. Une chaine d’exposition est compo...
