Mehalko, Jennifer Drew, Matthew Snead, Kelly Denson, John-Paul Wall, Vanessa Taylor, Troy Sadtler, Kaitlyn Messing, Simon Gillette, William Esposito, Dominic
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Published in
Protein expression and purification
The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is a commonly used antigen for serology assays critical to determining the extent of SARS-CoV-2 exposure in the population. Different versions of the RBD protein have been developed and utilized in assays, with higher sensitivity attributed to particular forms of the protein. To impr...
Breva, Silvia Montolio Clavero, Carmen Molina Bertomeu, Frederic Gómez Picó-Plana, Ester Orús, Núria Serrat Sánchez, Inmaculada Palau Prad, Maria Teresa Mestre Mateu, Maria Teresa Sans
Published in
Enfermedades Infecciosas Y Microbiologia Clinica
Introducción: Para hacer frente a la pandemia actual causada por el nuevo coronavirus SARS-CoV-2 se han desarrollado recientemente varios inmunoensayos serológicos con el objetivo de ser utilizados como herramienta diagnóstica complementaria y apoyar la técnica de RT-PCR actualmente considerada como el "estándar de oro". Sin embargo, estos nuevos e...
Tozetto-Mendoza, Tania Regina Kanunfre, Kelly Aparecida Vilas-Boas, Lucy Santos Sanchez Espinoza, Evelyn Patricia Paião, Heuder Gustavo Oliveira Rocha, Mussya Cisotto de Paula, Anderson Vicente de Oliveira, Maura Salaroli Zampelli, Daniella Bosco Vieira, José Mauro Jr
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Published in
Journal of Virological Methods
Objectives We evaluated the performance of a nucleoprotein-based enzyme-linked immunosorbent assay (ELISA) for detection of IgG antibodies to SARS-CoV-2. Methods The ELISA was based on serum IgG reactivity to a 46-kDa protein derived from the recombinant SARS-CoV2 nucleoprotein. Assay sensitivity was assessed using serum samples from 134 COVID-19 c...
Register, Karen B. Parker, Margaret Patyk, Kelly A. Sweeney, Steven J. Boatwright, William D. Jones, Lee C. Woodbury, Murray Hunter, David L. Treanor, John Kohr, Marshall
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Published in
BMC Veterinary Research
BackgroundMycoplasma bovis causes mastitis, otitis, pneumonia and arthritis in cattle and is a major contributor to bovine respiratory disease complex. Around the year 2000, it emerged as a significant threat to the health of North American bison. Whether healthy bison are carriers of M. bovis and when they were first exposed is not known. To inves...
Shen, Yuanxi Ji, Rongyi Chai, Rui Yuan, Nana Zhang, Jiyue Jing, Yi Yang, Man Zhang, Lanqi Hong, Yang Lin, Jiaojiao
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Parasites & Vectors
BackgroundSchistosomiasis japonica is a severe zoonosis. Domestic animals are the primary source of infection and play an important role in disease transmission. Surveillance and diagnosis play key roles in schistosomiasis control; however, current techniques for the surveillance and diagnosis of the disease have limitations. In this study, we deve...
Toker, Eda Baldan Yeşilbağ, Kadir
Published in
Tropical Animal Health and Production
The aim of this study was to evaluate the compatibility among virus isolation (VI), ELISA, and PCR for diagnosis of the major viral agents (BPIV-3, BRSV, BVDV, and BoHV-1) responsible for BRD in the field samples. For that purpose, a total of 193 samples (133 nasal swabs and 60 lung tissue samples) from cattle with respiratory signs in northwestern...
de Assis, Rafael R. Jain, Aarti Nakajima, Rie Jasinskas, Algis Felgner, Jiin Obiero, Joshua M. Norris, Philip J. Stone, Mars Simmons, Graham Bagri, Anil
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Published in
Nature Communications
COVID-19 diagnosis is commonly performed by PCR testing, however, serologic methods are more accurate and versatile for monitoring disease burden and epidemiology. Here the authors report a protein microarray with antigens from SARS-CoV-2, SARS-CoV, MERS-CoV as well as common human respiratory viruses.
Klumpp-Thomas, Carleen Kalish, Heather Drew, Matthew Hunsberger, Sally Snead, Kelly Fay, Michael P. Mehalko, Jennifer Shunmugavel, Anandakumar Wall, Vanessa Frank, Peter
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Published in
Nature Communications
Understanding the infection parameters and host responses against SARS-CoV-2 require data from large cohorts using standardized methods. Here, the authors optimize a serum ELISA protocol that has minimal cross-reactivity and flexible sample collection workflow in an attempt to standardize data generation and help inform on COVID-19 pandemic and imm...
Das, Rina Jobayer Chisti, Mohammod Ahshanul Haque, Md. Ashraful Alam, Md. Das, Subhasish Mahfuz, Mustafa Mondal, Dinesh Ahmed, Tahmeed
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Vaccine
Background Vaccine-preventable infectious diseases are often responsible for childhood morbidity and high rates of mortality. Immune response to the vaccine is associated with multiple factors in early childhood and measured by antibody titers. Among them, micronutrient deficiencies such as vitamin D and zinc deficiencies are the most important in ...
Paquet-Mercier, François Juncker, David Bergeron, Sébastien
Published in
Methods in molecular biology (Clifton, N.J.)
Common multiplex sandwich immunoassays suffer from cross-reactivity due to the mixing of detection antibodies and the combinatorial, undesired interaction between all reagents and analytes. Here we present the snap chip to perform antibody colocalization microarrays that eliminates undesirable interactions by running an array of singleplex assays r...