Torok, Justin Maia, Pedro D Anand, Chaitali Raj, Ashish
Neurodegenerative diseases such as Alzheimer's disease exhibit pathological changes in the brain that proceed in a stereotyped and regionally specific fashion. However, the cellular underpinnings of regional vulnerability are poorly understood, in part because whole-brain maps of a comprehensive collection of cell types have been inaccessible. Here...
Biswas, Pooja Woodard, DaNae R Hollingsworth, TJ Khan, Naheed W Lazaro, Danielle R Berry, Anne Marie Dagar, Manisha Pan, Yang Garland, Donita Shaw, Peter X
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The high-temperature requirement A1 (HTRA1), a serine protease, has been demonstrated to play a pivotal role in the extracellular matrix (ECM) and has been reported to be associated with the pathogenesis of age-related macular degeneration (AMD). To delineate its role in the retina, the phenotype of homozygous Htra1-KO (Htra1-/-) mice was character...
Zhu, Fengli Wang, Zhuqing Davis, Kayla McSwiggin, Hayden Zyuzin, Jekaterina Liu, Jie Yan, Wei Rehan, Virender K Jendzjowsky, Nicholas
BackgroundEpigenetic changes can be shaped by a wide array of environmental cues, maternal health, and behaviors. One of the most detrimental behaviors to the developing fetus is nicotine exposure. Perinatal nicotine exposure remains a significant risk factor for cardiovascular health and, in particular, hypertension. Increased basal carotid body (...
Duran, Pamela Zelus, Emma I Burnett, Lindsey A Christman, Karen L Alperin, Marianna
BackgroundVaginal childbirth is a key risk factor for pelvic floor muscle injury and dysfunction, and subsequent pelvic floor disorders. Multiparity further exacerbates these risks. Using the rat model, validated for the studies of the human pelvic floor muscles, we have previously identified that a single simulated birth injury results in pelvic f...
Özcan, Ezgi Yu, Kristie Dinh, Lyna Lum, Gregory Lau, Katie Hsu, Jessie Arino, Mariana Paramo, Jorge Lopez-Romero, Arlene Hsiao, Elaine
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The gut microbiome modulates the anti-seizure effects of the ketogenic diet, but how specific dietary formulations differentially modify the gut microbiome in ways that impact seizure outcome is poorly understood. We find that medical ketogenic infant formulas vary in macronutrient ratio, fat source, and fiber content and differentially promote res...
Zheng, Weihao Borja, Michael Dorman, Leah Liu, Jonathan Zhou, Andy Seng, Amanda Arjyal, Ritwicq Sunshine, Sara Nalyvayko, Alina Pisco, Angela
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Mycobacterium tuberculosis (MTB) ESX-1, a type VII secretion system, is a key virulence determinant contributing to MTBs survival within lung mononuclear phagocytes (MNPs), but its effect on MNP recruitment and differentiation remains unknown. Here, using multiple single-cell RNA sequencing techniques, we studied the role of ESX-1 in MNP heterogene...
Li, Zhengxing Guo, Zhongyuan Zhang, Fangyu Sun, Lei Luan, Hao Fang, Zheng Dedrick, Jeramy Zhang, Yichen Tang, Christine Zhu, Audrey
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Amidst the rising prevalence of respiratory diseases, the importance of effective lung treatment modalities is more critical than ever. However, current drug delivery systems face significant limitations that impede their efficacy and therapeutic outcome. Biohybrid microrobots have shown considerable promise for active in vivo drug delivery, especi...
Chen, Wei Kim, Seojin Kim, Sharon Beheshtian, Cheyenne Kim, Naryung Shin, Ki-Hyuk Kim, Reuben Kim, Sangjae Park, No-Hee
Doxorubicin is a highly effective anticancer agent, but its clinical use is restricted by severe side effects, including atherosclerosis and cardiomyopathy. These complications are partly attributed to doxorubicins ability to induce endothelial-to-mesenchymal transition (EndMT) in vascular endothelial cells, a critical process in the initiation and...
Biswas, Subarna Hilser, James Woodward, Nicholas Wang, Zeneng Gukasyan, Janet Nemet, Ina Schwartzman, William Huang, Pin Han, Yi Fouladian, Zachary
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Background: Circulating glycine levels have been associated with reduced risk of coronary artery disease (CAD) in humans but these associations have not been observed in all studies. We evaluated whether the relationship between glycine levels and atherosclerosis was causal using genetic analyses in humans and feeding studies in mice. Methods: Seru...
Hoover, Evelyn M Schneider, Christine A Crouzet, Christian Lima, Tatiane S Velez, Dario X Figueroa Tran, Cuong J Agalliu, Dritan Gandhi, Sunil P Choi, Bernard Lodoen, Melissa B
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BackgroundImmunothrombosis is the process by which the coagulation cascade interacts with the innate immune system to control infection. However, the formation of clots within the brain vasculature can be detrimental to the host. Recent work has demonstrated that Toxoplasma gondii infects and lyses central nervous system (CNS) endothelial cells tha...