Wang, Jiaxi Yan, Kun Wang, Wenya Zhou, Yan
Published in
Bioresources and Bioprocessing
BackgroundLignin’s valorization plays a critical role in refining the bioresource. Considering that the β-aryl ether linkage (β-O-4 bond) accounts for 50–70% of lignin chemical linkage between aromatic rings, the hydrolase of lignin β-aryl ether linkage, especially the β-etherase, provided a promising way for the lignin depolymerization and valoriz...
Ma, Chunling Liu, Meixia You, Chun Zhu, Zhiguang
Published in
Bioresources and Bioprocessing
BackgroundDiaphorase (DI) has received wide attention as the key anodic enzyme mediating the electron transfer and electric energy generation in enzymatic biofuel cells (EBFCs). Lowering the anodic pH may be a useful strategy for constructing high-performance in EBFCs. However, most DI suffered from the poor activity at low pHs. Therefore, it is ne...
Xu, Lian Wang, Li-Chao Su, Bing-Mei Xu, Xin-Qi Lin, Juan
Published in
Bioresource technology
L-threonine transaldolase(PsLTTA) could asymmetric synthesize β-hydroxy-α-amino acids (HAAs) with excellentstereoselectivity, while the poor yield limited its further application. Here we provided a combinatorial strategy to improve HAAs production, by directed evolution of PsLTTA towards enhanced activity and introducing an acetaldehyde eliminatio...
Jia, Xiaoqiang Ma, Yubing Bu, Rongrong Zhao, Tingting Wu, Kang
Published in
AMB Express
Directed evolution has been proven as a powerful tool for developing proteins and strains with novel or enhanced features. In this study, a dual selection system was designed to tune the binding specificity of a transcription factor to a particular ligand with the ampicillin resistance gene amp (ON selection) as the positive selection marker and th...
Mirzadeh, Kiavash Shilling, Patrick J. Elfageih, Rageia Cumming, Alister J. Cui, Huanhuan L. Rennig, Maja Nørholm, Morten H. H. Daley, Daniel O.
Published in
Microbial Cell Factories
BackgroundRecombinant proteins are often engineered with an N-terminal signal peptide, which facilitates their secretion to the oxidising environment of the periplasm (gram-negative bacteria) or the culture supernatant (gram-positive bacteria). A commonly encountered problem is that the signal peptide influences the synthesis and secretion of the r...
Lee, Misun Rozeboom, Henriëtte J. Keuning, Eline de Waal, Paul Janssen, Dick B.
Published in
Biotechnology for Biofuels
BackgroundEfficient bioethanol production from hemicellulose feedstocks by Saccharomyces cerevisiae requires xylose utilization. Whereas S. cerevisiae does not metabolize xylose, engineered strains that express xylose isomerase can metabolize xylose by converting it to xylulose. For this, the type II xylose isomerase from Piromyces (PirXI) is used ...
Zhang, Xiaodong Peng, Yaqin Zhao, Jing Li, Qian Yu, Xiaojuan Acevedo-Rocha, Carlos G. Li, Aitao
Published in
Bioresources and Bioprocessing
Steroids are the most widely marketed products by the pharmaceutical industry after antibiotics. Steroid hydroxylation is one of the most important functionalizations because their derivatives enable a higher biological activity compared to their less polar non-hydroxylated analogs. Bacterial cytochrome P450s constitute promising biocatalysts for s...
Ren, Chang Wen, Xin Mencius, Jun Quan, Shu
Published in
Bioresources and Bioprocessing
Protein stability is not only fundamental for experimental, industrial, and therapeutic applications, but is also the baseline for evolving novel protein functions. For decades, stability engineering armed with directed evolution has continued its rapid development and inevitably poses challenges. Generally, in directed evolution, establishing a re...
Stellwagen, Sarah D. Sarkes, Deborah A. Adams, Bryn L. Hunt, Mia A. Renberg, Rebecca L. Hurley, Margaret M. Stratis-Cullum, Dimitra N.
Published in
BMC Biotechnology
BackgroundBacterial surface display libraries are a popular tool for novel ligand discovery due to their ease of manipulation and rapid growth rates. These libraries typically express a scaffold protein embedded within the outer membrane with a short, surface-exposed peptide that is either terminal or is incorporated into an outer loop, and can the...
Sulej, Agata A.
Published in
BMC Research Notes
ObjectiveType C2H2 zinc fingers bind a variety of substrates, specific sequences in the double-stranded DNA counting among them. Engineering efforts led to the discovery of a set of general rules that enable obtaining zinc fingers modules that bind to almost any given sequence. The objective of this work was to determine an analogical set of rules ...
