Sun, Qi-Fan Zheng, Yu-Cong Chen, Qi Xu, Jian-He Pan, Jiang
Published in
Biochemical and biophysical research communications
Oleate hydratase catalyzes the hydration of unsaturated fatty acids, giving access to C10-functionalization of oleic acid. The resultant 10-hydroxystearic acid is a key material for the synthesis of many biomass-derived value-added products. Herein, we report the engineering of an oleate hydratase from Paracoccus aminophilus (PaOH) with significant...
Runthala, Ashish Sai, Tavakala Harsha Kamjula, Vandana Phulara, Suresh C. Rajput, Vikrant Singh Sangapillai, Karthikeyan
Published in
Journal of Genetic Engineering and Biotechnology
BackgroundTo achieve a high yield of terpenoid-based therapeutics, 1-deoxy-d-xylulose-5-phosphate (DXP) pathway has been significantly exploited for the production of downstream enzymes. The DXP synthase (DXS) enzyme, the initiator of this pathway, is pivotal for the convergence of carbon flux, and is computationally studied well for the industrial...
Liu, Xiutao Feng, Xinjun Ding, Yamei Gao, Wenjie Xian, Mo Wang, Jichao Zhao, Guang
Published in
Metabolic engineering
Propionyl-CoA carboxylase (PCC) is a promising enzyme in the fields of biological CO2 utilization, synthesis of natrual products, and so on. The activity and substrate specificity of PCC are dependent on its key subunit carboxyltransferase (CT). To obtain PCC with high enzyme activity, seven pccB genes encoding CT subunit from diverse microorganism...
Liu, Yilan Chen, Jinjin Khusnutdinova, Anna N. Correia, Kevin Diep, Patrick Batyrova, Khorcheska A. Nemr, Kayla Flick, Robert Stogios, Peter Yakunin, Alexander F.
...
Published in
Biotechnology for Biofuels
BackgroundAldehyde decarbonylases (ADs), which convert acyl aldehydes into alkanes, supply promising solution for producing alkanes from renewable feedstock. However the instability of ADs impedes their further application. Therefore, the current study aimed to investigate the degradation mechanism of ADs and engineer it towards high stability.Resu...
Viña-Gonzalez, Javier Alcalde, Miguel
Published in
Computational and Structural Biotechnology Journal
Aryl-alcohol oxidase (AAO) is a fungal GMC flavoprotein secreted by white-rot fungi that supplies H2O2 to the ligninolytic consortium. This enzyme can oxidize a wide array of aromatic alcohols in a highly enantioselective manner, an important trait in organic synthesis. The best strategy to adapt AAO to industrial needs is to engineer its propertie...
Ilić Đurđić, Karla Ece, Selin Ostafe, Raluca Vogel, Simon Balaž, Ana Marija Schillberg, Stefan Fischer, Rainer Prodanović, Radivoje
Published in
Journal of bioscience and bioengineering
Lignin peroxidase (LiP) is a heme-containing oxidoreductase that oxidizes structurally diverse substrates in an H2O2-dependent manner. Its ability to oxidize many pollutants makes it suitable for bioremediation applications and an ideal candidate for optimization by mutagenesis and selection. In order to increase oxidative stability of LiP we gener...
Wang, Jiaxi Yan, Kun Wang, Wenya Zhou, Yan
Published in
Bioresources and Bioprocessing
BackgroundLignin’s valorization plays a critical role in refining the bioresource. Considering that the β-aryl ether linkage (β-O-4 bond) accounts for 50–70% of lignin chemical linkage between aromatic rings, the hydrolase of lignin β-aryl ether linkage, especially the β-etherase, provided a promising way for the lignin depolymerization and valoriz...
Ma, Chunling Liu, Meixia You, Chun Zhu, Zhiguang
Published in
Bioresources and Bioprocessing
BackgroundDiaphorase (DI) has received wide attention as the key anodic enzyme mediating the electron transfer and electric energy generation in enzymatic biofuel cells (EBFCs). Lowering the anodic pH may be a useful strategy for constructing high-performance in EBFCs. However, most DI suffered from the poor activity at low pHs. Therefore, it is ne...
Xu, Lian Wang, Li-Chao Su, Bing-Mei Xu, Xin-Qi Lin, Juan
Published in
Bioresource technology
L-threonine transaldolase(PsLTTA) could asymmetric synthesize β-hydroxy-α-amino acids (HAAs) with excellentstereoselectivity, while the poor yield limited its further application. Here we provided a combinatorial strategy to improve HAAs production, by directed evolution of PsLTTA towards enhanced activity and introducing an acetaldehyde eliminatio...
Jia, Xiaoqiang Ma, Yubing Bu, Rongrong Zhao, Tingting Wu, Kang
Published in
AMB Express
Directed evolution has been proven as a powerful tool for developing proteins and strains with novel or enhanced features. In this study, a dual selection system was designed to tune the binding specificity of a transcription factor to a particular ligand with the ampicillin resistance gene amp (ON selection) as the positive selection marker and th...
