Kaplan, Mehmet Mahsum Flucher, Bernhard E
Published in
iScience
Activity-dependent calcium signals in developing muscle play a crucial role in neuromuscular junction (NMJ) formation. However, its downstream effectors and interactions with other regulators of pre- and postsynaptic differentiation are poorly understood. Here, we demonstrate that the skeletal muscle calcium channel CaV1.1 and β-catenin interact in...
Degond, Pierre Hecht, Sophie Romanos, Michèle Trescases, Ariane
We introduce two 2D mechanical models reproducing the evolution of two viscous tissues in contact. Their main property is to model the swirling cell motions while keeping the tissues segregated, as observed during vertebrate embryo elongation. Segregation is encoded differently in the two models: by passive or active segregation (based on a mechani...
Velloso, Fernando Janczur Kumari, Ekta Buono, Krista D Frondelli, Michelle J Levison, Steven W
Published in
STAR protocols
This protocol describes an ex vivo approach to identify and quantify the proportions of proliferating neural stem cells and progenitors of the mouse subventricular zone. It uses ethynyl deoxyuridine (EdU) incorporation to identify dividing cells, combined with multicolor flow cytometry for 4 cell surface antigens to distinguish between 8 phenotypic...
Hernandez-Huertas, Luis Kushawah, Gopal Diaz-Moscoso, Alejandro Tomas-Gallardo, Laura Moreno-Sanchez, Ismael da Silva Pescador, Gabriel Bazzini, Ariel A Moreno-Mateos, Miguel A
Published in
STAR protocols
CRISPR-Cas systems have been used to induce DNA mutagenesis for gene function discovery. However, the development of tools to eliminate RNAs provides complementary and unique approaches to disrupt gene expression. Here, we present a workflow to perform specific, efficient, and cost-effective mRNA knockdown in zebrafish embryos using our in vivo opt...
Wang, Mengzhe Misgeld, Thomas Brill, Monika S
Published in
STAR protocols
This step-by-step protocol provides a fast and easy technique to label and/or genetically manipulate neural cells, achieved by intraventricular injection of viral vectors into neonatal mice under ultrasound guidance. Successful injection of adeno-associated viral vectors (AAV) induces neural transduction as fast as 3 days post injection (dpi) in bo...
Watanabe-Takano, Haruko Fukumoto, Moe Fukuhara, Shigetomo Mochizuki, Naoki
Published in
STAR protocols
Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in reflecting endogenous expression of genes of interest in the whole body can be visualized by X-gal staining. This protocol is suitable for examining the developmental stage-specific expression of genes of in...
Ruiz, Oscar E Samms, Krystin M Eisenhoffer, George T
Published in
STAR protocols
Epithelial tissues sustain barrier function by removing and replacing aberrant or unfit cells. Here, we describe approaches to evaluate epithelial restorative capacity after inducing cell loss in zebrafish larvae. We provide details to quantify morphological changes to the tail fin epithelium after cell loss, and instructions to interrogate changes...
Zhu, Xiyu Grace, Anthony A
Published in
STAR protocols
Here, we present a protocol for delivering environmental enrichment (EE) in discrete postnatal windows to prevent long-term dopamine neuron dysfunctions in a neurodevelopmental rat model of schizophrenia risk. We describe generation of the schizophrenia model through prenatal treatment of rats with methylazoxymethanol acetate (the MAM model) and th...
Braun, Tatum W Kuoch, Michael K Khandros, Eugene Li, Hojun
Published in
STAR protocols
Early erythroid progenitors are transit-amplifying cells with high proliferative capacity committed to undergoing red cell differentiation. CD71/CD24low progenitors are less mature and have greater proliferative capacity than CD71/CD24high. We present protocols for isolation of CD71/CD24low progenitors from mouse fetal liver using both fluorescence...
Kleist, Thomas J Bortolazzo, Anthony Keyser, Zachary P Perera, Adele M Irving, Thomas Venkateshwaran, Muthusubramanian Atanjaoui, Fatiha Tang, Ren-Jie Maeda, Junko Cartwright, Heather N
...
Symbioses between angiosperms and rhizobia or arbuscular mycorrhizal fungi are controlled through a conserved signaling pathway. Microbe-derived, chitin-based elicitors activate plant cell surface receptors and trigger nuclear calcium oscillations, which are decoded by a calcium/calmodulin-dependent protein kinase (CCaMK) and its target transcripti...
