Pieles, Oliver Reichert, Torsten E. Morsczeck, Christian
Published in
Stem Cell Research & Therapy
BackgroundHuman dental follicle cells (DFCs) are the precursor cells of the periodontium with a high potential for regenerative therapies of (alveolar) bone. However, the molecular mechanisms of osteogenic differentiation are inadequately understood. Classical isoforms of protein kinase C (PKC) are reported to inhibit osteogenesis of stem/precursor...
Pieles, Oliver Hartmann, Maria Morsczeck, Christian
Published in
Archives of oral biology
Dental follicle cells (DFCs) are progenitors of alveolar osteoblasts. AMP-activated protein kinase (AMPK) and the down-stream activated autophagy process play a key role in cellular energy and metabolic homeostasis and are involved in many biological processes including differentiation. Previous studies showed ambiguous results about the role of AM...
Wang, Qi Yuan, Xiaojuan Li, Boqi Sun, Dalei Liu, Jia Liu, Tao Bi, Xiaojuan Liu, Yishan
Published in
Molecular Biology Reports
ObjectiveTo investigate the expression of C-JNK, RANKL and OPG after SP600125 administration in cultured dental follicle cells (DFCs).MethodsTRAP staining and electron microscope were carried out on day 7 and 9 after coculture of BMMs and DFCs with a ratio of 5:1 in different groups. To determine the effects of SP600125 on the expression of C-JNK, ...
Morsczeck, Christian Reck, Anja Reichert, Torsten E.
Published in
Molecular and Cellular Biochemistry
The osteogenic differentiation of dental follicle cells (DFCs) is inhibited by the onset of cellular senescence, but the cause for this is largely unknown. Recently it was shown that WNT5a, which is an inductor of the non-canonical WNT pathway, stimulates both cellular senescence and osteogenic differentiation of different cell types. In this study...
Morsczeck, Christian Reck, Anja Reichert, Torsten E.
Published in
BMC Molecular and Cell Biology
BackgroundDental follicle cells (DFCs) are dental stem cells and interesting options for regenerative therapies in dentistry. However, DFCs acquire replicative senescence in long-term cultures, but little is known about molecular processes. In previous studies, we observed that DFC cell lines become senescent at different rates. We hypothesized tha...
Morsczeck, Christian Hullmann, Markus Reck, Anja Reichert, Torsten E.
Published in
Molecular and Cellular Biochemistry
Cellular senescence is a restricting factor for regenerative therapies with somatic stem cells. We showed previously that the onset of cellular senescence inhibits the osteogenic differentiation in stem cells of the dental follicle (DFCs), although the mechanism remains elusive. Two different pathways are involved in the induction of the cellular s...
Morsczeck, C. Reck, A. Beck, H. C.
Published in
Molecular and Cellular Biochemistry
Dental follicle stem cells (DFCs) are precursor cells of alveolar osteoblasts, and previous studies have shown that the growth factor bone morphogenetic protein (BMP)2 induces the osteogenic differentiation of DFCs. However, the molecular mechanism down-stream of the induction of the osteogenic differentiation by BMP2 remains elusive. We investigat...
Klingelhöffer, C. Reck, A. Morsczeck, C.
Published in
Cytotechnology
The secretion of osteocalcin (OCN) is an excellent differentiation marker for the osteogenic differentiation. This study investigated the secretion of OCN during the osteogenic differentiation of DFCs. During the differentiation of DFCs the extracellular concentrations of OCN were higher in standard cell culture medium than in osteogenic differenti...
Sakisaka, Yukihiko Kanaya, Sousuke Nakamura, Takashi Tamura, Masato Shimauchi, Hidetoshi Nemoto, Eiji
Published in
Biochemical and biophysical research communications
Wnt3a is a secreted glycoprotein that activates the glycogen synthase kinase-3β (GSK3β)/β-catenin signaling pathway through low-density-lipoprotein receptor-related protein (LRP)5/6 co-receptors. Wnt3a has been implicated in periodontal development and homeostasis, as well as in cementum formation. Recently, we have reported that Wnt3a increases al...
Morsczeck, Christian Gresser, Jan Ettl, Tobias
Published in
Molecular and Cellular Biochemistry
Dental stem cells such as human dental follicle cells (DFCs) have opened new promising treatment alternatives for today’s dental health issues such as periodontal tissue regeneration. However, cellular senescence represents a restricting factor to cultured stem cells, resulting in limited lifespan and reduced cell differentiation potential. Therefo...
