Développement de sources supercontinuum et applications biomédicales
Dynamic interaction of injected liquid jet with skin layer interfaces revealed by microsecond imaging of optically clear...
Published in Journal of Biological Engineering
Background Needle-free jet injection (NFJI) systems enable a controlled and targeted delivery of drugs into skin tissue. However, a scarce understanding of their underlying mechanisms has been a major deterrent to the development of an efficient system. Primarily, the lack of a suitable visualization technique that could capture the dynamics of the...
Recent advances in optical imaging through deep tissue: imaging probes and techniques
Published in Biomaterials Research
Optical imaging has been essential for scientific observations to date, however its biomedical applications has been restricted due to its poor penetration through tissues. In living tissue, signal attenuation and limited imaging depth caused by the wave distortion occur because of scattering and absorption of light by various molecules including h...
Organelle-specific blue-emitting two-photon probes for calcium ions: Combination with green-emitting two-photon probe fo...
Published in Talanta
In this study, we developed organelle-specific blue-emitting two-photon (TP) probes for Ca2+ (BCa-1, BCa-2mito, and BCa-3mem), with absorption maxima (λmax) at 350-358 nm, emission maxima (λfl) at 464-466 nm, and TP action cross-section (Φδmax) values of 55-70 × 10-50 cm4s/photon, in the presence of excess Ca2+ at 750 nm. Moreover, the probes had d...
Multiphoton Deep-Tissue Imaging of Micrometastases and Disseminated Cancer Cells Using Conjugates of Quantum Dots and Si...
Early detection of malignant tumors, micrometastases, and disseminated tumor cells is one of the effective way of fighting cancer. Among the many existing imaging methods like computed tomography (CT), ultrasound (US), magnetic resonance imaging (MRI), positron emission tomography (PET), and single-photon emission computed tomography (SPECT), optic...
Deep tissue photoacoustic imaging of nickel(II) dithiolene-containing polymeric nanoparticles in the second near-infrare...
Published in Theranostics
Photoacoustic imaging is gaining great attention in the medical world due to its significant potential for clinical translation. Light excitation in the second near-infrared (NIR-II) window (1000-1350 nm) has resolution and penetration depth suitable for several clinical applications. However, the significant challenge exists for clinical translati...
Deep tissue photoacoustic imaging of nickel(II) dithiolene-containing polymeric nanoparticles in the second near-infrare...
Photoacoustic imaging is gaining great attention in the medical world due to its significant potential for clinical translation. Light excitation in the second near-infrared (NIR-II) window (1000-1350 nm) has resolution and penetration depth suitable for several clinical applications. However, the significant challenge exists for clinical translati...
Application of a cybLuc Aminoluciferin for Deep Tissue Bioluminescence Imaging in Rodent Models.
Published in Methods in molecular biology (Clifton, N.J.)
Bioluminescent imaging (BLI) technology has been extensively applied due to various advantages such as noninvasiveness, high sensitivity and selectivity, excellent biocompatibility and real-time visualization and monitoring. The firefly luciferase (Fluc)/luciferin system, one of the principal bioluminescent systems, has been developed as a sensor f...
A mitochondria-targeted ratiometric two-photon fluorescent probe for detecting intracellular cysteine and homocysteine.
Published in Talanta
A novel mitochondria-targeted ratiometric two-photon fluorescent probe (Mito-MQ) for detecting intracellular cysteine (Cys) and homocysteine (Hcy) has been designed. Mito-MQ showed the ratiometric fluorescent detection signal (the green-to-blue emissionfrom 517nm to 460nm) to cysteine (Cys) and homocysteine (Hcy) over glutathione (GSH), along with ...
Optical Brain Imaging: A Powerful Tool for Neuroscience
Published in Neuroscience Bulletin
As the control center of organisms, the brain remains little understood due to its complexity. Taking advantage of imaging methods, scientists have found an accessible approach to unraveling the mystery of neuroscience. Among these methods, optical imaging techniques are widely used due to their high molecular specificity and single-molecule sensit...
