Christopher, Heavenlight Burns, Adam Josephat, Emmanuel Makani, Julie Schuh, Anna Nkya, Siana
Published in
BMC Genomics
BackgroundSickle cell disease (SCD) is an important cause of under-five mortality. Tanzania is the 5th country in the world with the highest births prevalence of SCD individuals. Significant advances in the neonatal diagnosis of SCD using rapid point-of-care testing have been made. However genetic confirmation is still required for positive cases, ...
Bocharova, D. V. Alekseev, Ya. I. Volkov, A. A. Lavrov, G. S. Plugov, A. G. Volkov, I. A. Chemigov, A. A. Bardin, B. V. Kurochkin, V. E.
Published in
Journal of Analytical Chemistry
AbstractDNA sequencing is an essential task of modern molecular genetic research. Its fundamental parameter is the accuracy of decoding. In this work, we determined the maximum lengths of DNA fragments, obtained by the Sanger method and sequenced with an accuracy of 99%, in a polymer based on linear poly(N,N-dimethylacrylamide) PDMA-6 for capillari...
Harhay, Gregory P Harhay, Dayna M Brader, Kerry D Smith, Timothy P L
Published in
Microbiology spectrum
Histophilus somni is a Gram-negative bacterial organism that acts as an opportunistic pathogen and is a fastidious member of the Pasteurellaceae family associated with diseases of respiratory, reproductive, cardiac, and other tissues of ruminants. We identified an intervening sequence (IVS) embedded in all five copies of the 23S rRNA gene in the cl...
Malonga, Gervillien Arnold Jary, Aude Leducq, Valentin Moudiongui Mboungou Malanda, Dimitry Boumba, Anicet Luc Magloire Chicaud, Elodie Malet, Isabelle Calvez, Vincent Peko, Jean Felix Marcelin, Anne-Geneviève
...
Published in
Scientific Reports
Human herpesvirus 8 (HHV8) is endemic in Africa, although studies of this infection are rare in Congo. We evaluated seroprevalence and HHV-8 diversity among people living with HIV. We included 353 patients receiving highly active antiretroviral therapy. Antibodies against HHV-8 latency-associated nuclear antigen were detected by indirect immunofluo...
Pellegrino, Eric Jacques, Coralie Beaufils, Nathalie Nanni, Isabelle Carlioz, Antoine Metellus, Philippe Ouafik, L’Houcine
Published in
Scientific Reports
Since 2017, we have used IonTorrent NGS platform in our hospital to diagnose and treat cancer. Analyzing variants at each run requires considerable time, and we are still struggling with some variants that appear correct on the metrics at first, but are found to be negative upon further investigation. Can any machine learning algorithm (ML) help us...
Pérez-Hernández, Valentín Hernández-Guzmán, Mario Valenzuela-Encinas, César Alcántara-Hernández, Rocío Estrada-Alvarado, Isabel Dendooven, Luc Marsch, Rodolfo Gutiérrez-Miceli, Federico Ruíz-Valdiviezo, Víctor M. Montes-Molina, Joaquín A.
...
Summary: A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (Mo Bio™ Laboratories, Inc.) was used as a reference technique. The method reported her...
Antonín, Vladimír Ďuriška, Ondrej Jančovičová, Soňa Para, Roberto Kudláček, Tomáš Tomšovský, Michal
Published in
Mycologia
Revision of the taxonomically complicated European Melanoleuca taxa with well-developed macrocystidia (subgenus Melanoleuca) is presented. The species are delimited by macro- and micromorphological characters and by multilocus molecular data based on internal transcribed spacer (ITS), second largest subunit of RNA polymerase I (rpb2), and translati...
Wang, Chen Maeda, Masakazu Chen, Jian Wang, Mengxue Naruse, Keiji Takahashi, Ken
The method presented in this article are related to the research article entitled as "Role of the TRPM4 channel in mitochondrial function, calcium release, and ROS generation in oxidative stress" [1]. TRPM4, a non-selective monovalent cation channel, is not only involved in the generation of the action potential in cardiomyocytes, but also thought ...
Okada, Yukinori Wang, Qingbo S
Published in
Nature
Yuan, Yuxuan Bayer, Philipp E Batley, Jacqueline Edwards, David
Published in
Plant biotechnology journal
Structural variations (SVs) including gene presence/absence variations and copy number variations are a common feature of genomes in plants and, together with single nucleotide polymorphisms and epigenetic differences, are responsible for the heritable phenotypic diversity observed within and between species. Understanding the contribution of SVs t...
