Piazza, Aurèle Rajput, Pallavi Heyer, Wolf-Dietrich
Published in
Methods in molecular biology (Clifton, N.J.)
DNA double-strand breaks (DSBs) are genotoxic lesions that can be repaired in a templated fashion by homologous recombination (HR). HR is a complex pathway that involves the formation of DNA joint molecules (JMs) containing heteroduplex DNA. Various types of JMs are formed throughout the pathway, including displacement loops (D-loops), multi-invasi...
Arnould, Coline Rocher, Vincent Legube, Gaëlle
Published in
Methods in molecular biology (Clifton, N.J.)
Among the types of damage, DNA double-strand breaks (DSBs) (provoked by various environmental stresses, but also during normal cell metabolic activity) are the most deleterious, as illustrated by the variety of human diseases associated with DSB repair defects. DSBs are repaired by two groups of pathways: homologous recombination (HR) and nonhomolo...
Eckenroth, Brian E Cao, Vy Bao Averill, April M Dragon, Julie A Doublié, Sylvie
Published in
Structure (London, England : 1993)
Oxidative damage on DNA arising from both endogenous and exogenous sources can result in base modifications that promote errors in replication as well as generating sites of base loss (abasic sites) that present unique challenges to maintaining genomic integrity. These lesions are excised by DNA glycosylases in the first step of the base excision r...
Liu, Jinhui Jiang, Pinping Chen, Xucheng Shen, Yujie Cui, Guoliang Ma, Ziyan Zhao, Shaojie Zhang, Yan
Published in
BMC Cancer
BackgroundEndometrial cancer (EC) is one of the most common gynecological malignancies worldwide. However, the molecular mechanisms and the prognostic prediction for EC patients remain unclear.MethodsIn the current study, we performed an in-depth analysis of over 500 patients which were obtained from the Cancer Genome Atlas (TCGA) database. The bio...
Carreira, Raquel Aguado, F Javier Lama-Diaz, Tomas Blanco, Miguel G
Published in
Methods in molecular biology (Clifton, N.J.)
Holliday junctions are four-way DNA structures that may arise during meiotic recombination, double-strand break repair, or postreplicative repair by the reciprocal exchange of single strands between two DNA molecules. Given their ability to effectively bridge two sister chromatids or homologous chromosomes, cells have implemented various pathways t...
Joseph, Fraulin Lee, So Jung Bryant, Eric Edward Rothstein, Rodney
Published in
Methods in molecular biology (Clifton, N.J.)
The precise organization of the genome inside the cell nucleus is vital to many cell functions including gene expression, cell division, and DNA repair. Here we describe a method to measure pairing of DNA loci during homologous recombination (HR) at a site-specific double-strand break (DSB) in Saccharomyces cerevisiae. This method utilizes a chromo...
Briest, Franziska Koziolek, Eva J Albrecht, Jakob Schmidt, Fränze Bernsen, Monique R Haeck, Joost Kühl, Anja A Sedding, Dagmar Hartung, Teresa Exner, Samantha
...
Published in
Neoplasia (New York, N.Y.)
Well-differentiated gastroenteropancreatic neuroendocrine neoplasms are rare tumors with a slow proliferation. They are virtually resistant to many DNA-damaging therapeutic approaches, such as chemo- and external beam therapy, which might be overcome by DNA damage inhibition induced by proteasome inhibitors such as bortezomib. In this study, we ass...
Zhou, Yi Paull, Tanya T
Published in
Methods in molecular biology (Clifton, N.J.)
DNA double-strand break (DSB) end resection initiates homologous recombination (HR) and is critical for genomic stability. DSB resection has been monitored indirectly in mammalian cells using detection of protein foci or BrdU foci formation, which is dependent on single-stranded DNA (ssDNA) products of resection. Here we describe a quantitative PCR...
Wong, Nancy John, Sam Nussenzweig, André Canela, Andrés
Published in
Methods in molecular biology (Clifton, N.J.)
DNA double-strand breaks (DSBs) represent the most toxic form of DNA damage and can arise in either physiological or pathological conditions. If left unrepaired, these DSBs can lead to genome instability which serves as a major driver to tumorigenesis and other pathologies. Consequently, localizing DSBs and understanding the dynamics of break forma...
Gaurav, Sachin Ranjan, Rajeev Kuldeep, Jitendra Dhiman, Kanika Mahapatra, Pinaki Prasad Ashish, Siddiqi, Mohammad Imran Ahmed, Shakil
Published in
Biochimica et biophysica acta. General subjects
Defects in DNA repair pathway can lead to double-strand breaks leading to genomic instability. Earlier we have shown that S.pombe Drp1, a Rint1/Tip1 family protein is required for the recovery from DNA damage. Various truncations of Drp1 protein were constructed and their role in DNA damage response and interaction with Rad50 protein has been studi...
