Chandrasekaran, Arun Richard
Published in
Nature Reviews. Chemistry
DNA nanostructures are increasingly used in biological applications, in which nuclease resistance is a key parameter. This Review discusses the different strategies used to modulate and evaluate the nuclease resistance of DNA nanostructures.
Khan, Muhammad Isa Gulzar, Salma Majid, Abdul Noor, Irum
Published in
Journal of Molecular Modeling
Deoxyribonucleic acid (DNA) drug intercalation is a well-known phenomenon for the treatment of cancer. Streptozotocin (STZ) is a drug agent containing toxic properties that make it good in the pancreatic cancer. The main objective of this study is the intercalation of the anticancer drug into the stacked base pair of DNA sequence with ATGC using a ...
Hiraga, Chiho Yamamoto, Satoshi Hashimoto, Sadamitsu Kasahara, Masataka Minamisawa, Tamiko Matsumura, Sachiko Katakura, Akira Yajima, Yasutomo Nomura, Takeshi Shiba, Kiyotaka
...
Published in
Scientific Reports
Oral fluids (OFs) contain small extracellular vesicles (sEVs or exosomes) that carry disease-associated diagnostic molecules. However, cells generate extracellular vesicles (EVs) other than sEVs, so the EV population is quite heterogeneous. Furthermore, molecules not packaged in EVs can also serve as diagnostic markers. For these reasons, developin...
Laifi, Anouar
This work presents an electrochemical DNA sensor microarray which supports the detection principles cyclic voltammetry (CV) and Coulometry. The three-electrode system needed for these detection methods is post-processed on the solid-state 5.5 mm x 3 mm CMOS-based chip, which is realized in a 180 nm, 1.8 V and 3.3 V standard CMOS technology. The con...
Li, Wenjing Zhang, Tianlong Sun, Mingliang Shi, Yu Zhang, Xiao-Jie Xu, Guo-Liang Ding, Jianping
Published in
Nature Communications
C5-glyceryl-methylcytosine is a DNA modification that plays a role in the regulation of green alga photosynthesis and is catalysed by CMD1, using vitamin C (VC) as a co-substrate. Here, the authors provide insights into the catalytic mechanism of CMD1 by determining the crystal structures of apo CMD1 and CMD1 bound to either VC or DNA, as well as t...
Dong, Wenjing Song, Erqun Song, Yang
Published in
Scientific Reports
The acute liver injury (ALI) and hepatic fibrosis caused by the co-treatment of lipopolysaccharide (LPS)/d-galactosamine (D-GalN) have been extensively studied. However, whether LPS/D-GalN are genotoxic has been left unknown. In this study, male mice were divided into eight groups with eight animals in each group. For acute challenge of LPS/D-GalN,...
Bartas, Martin Červeň, Jiří Guziurová, Simona Slychko, Kristyna Pečinka, Petr
Published in
International Journal of Molecular Sciences
Nucleic acid-binding proteins are traditionally divided into two categories: With the ability to bind DNA or RNA. In the light of new knowledge, such categorizing should be overcome because a large proportion of proteins can bind both DNA and RNA. Another even more important features of nucleic acid-binding proteins are so-called sequence or struct...
Purwidyantri, Agnes Domingues, Telma Borme, Jérôme Guerreiro, Joana Rafaela Ipatov, Andrey Abreu, Catarina M. Martins, Marco Alpuim, Pedro Prado, Marta
Published in
Biosensors
Liquid-gated Graphene Field-Effect Transistors (GFET) are ultrasensitive bio-detection platforms carrying out the graphene’s exceptional intrinsic functionalities. Buffer and dilution factor are prevalent strategies towards the optimum performance of the GFETs. However, beyond the Debye length (λD), the role of the graphene-electrolytes’ ionic spec...
Shende, Pravin Trivedi, Riddhi
Published in
Stem Cell Reviews and Reports
Layer-by-layer deposition of cells, tissues and similar molecules provided by additive manufacturing techniques such as 3D bioprinting offers safe, biocompatible, effective and inert methods for the production of biological structures and biomimetic scaffolds. 3D bioprinting assisted through computer programmes and software develops mutli-modal nan...
Yaffe, Nurit Rotem, Dvir Soni, Awakash Porath, Danny Shlomai, Joseph
Published in
Scientific Reports
Condensation and remodeling of nuclear genomes play an essential role in the regulation of gene expression and replication. Yet, our understanding of these processes and their regulatory role in other DNA-containing organelles, has been limited. This study focuses on the packaging of kinetoplast DNA (kDNA), the mitochondrial genome of kinetoplastid...