Published in Scientific Reports
Antibody-Oligonucleotide Conjugates (AOCs) represent an emerging class of functionalized antibodies that have already been used in a wide variety of applications. While the impact of dye and drug conjugation on antibodies’ ability to bind their target has been extensively studied, little is known about the effect caused by the conjugation of hydrop...
Published in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
V okviru magistrskega dela smo se osredotočili na s purini bogate oligonukleotide DNA, ki vsebujejo nukleotidna zaporedja 5'-d(CAGAGG)-3'. Tandemske ponovitve tega zaporedja pri replikaciji človeške genomske DNA tvorijo slabo raziskane sekundarne strukture, ki se tekom podvajanja ne morejo razviti in posledično pride do kolapsa replikacijskih vilic...
Published in BMC Research Notes
ObjectiveVisualisation methods, primarily color-coded representation of sequence data, have been a predominant means of representation of DNA data. Algorithmic conversion of DNA sequence data to sound—sonification—represents an alternative means of representation that uses a different range of human sensory perception. We propose that sonification ...
Published in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
TRIzol is a monophasic solution of phenol and guanidine isothiocyanate used for the extraction of RNA, DNA and proteins from tissues or cells. However, few studies have described its application to DNA extraction due to its time-consuming procedure. We present a TRIzol-modified method of extracting DNA from tissues using the TRIzol reagent and a si...
Hydration of biomolecules is an important physiological process that governs their structure, stability, and function. Herein, we probe the microhydration structure of cationic pyrimidine (Pym), a common building block of DNA/RNA bases, by infrared photodissociation spectroscopy (IRPD) of mass-selected microhydrated clusters, Pym+-Wn (W=H2O), in th...
Published in BMC Bioinformatics
BackgroundTumors are composed by a number of cancer cell subpopulations (subclones), characterized by a distinguishable set of mutations. This phenomenon, known as intra-tumor heterogeneity (ITH), may be studied using Copy Number Aberrations (CNAs). Nowadays ITH can be assessed at the highest possible resolution using single-cell DNA (scDNA) sequen...
Published in European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
Gold(III) complexes have been studied for the past years due to their anticancer properties and great affinity to biotargets, such as enzymes and proteins, which support their pharmacological applications. Within this scope, in this work the antiproliferative activities of two Au(III)-thiosemicarbazonate complexes, [AuClL1] (1, L1: (E,Z)-N-ethyl-N'...
Published in Biotechnic & histochemistry : official publication of the Biological Stain Commission
The mutation status of KRAS is important for anti-EGFR therapy in colorectal cancer (CRC) patients; however, detection of KRAS mutations in circulating tumor DNA (ctDNA) is problematic. We investigated tissue and plasma assays for KRAS mutations in CRC patients. The KRAS status of 407 CRC patients was evaluated using integration of amplification re...
Published in Frontiers in Immunology
