Published in Cancers
Simple Summary Recently, several molecules have improved the clinical outcome of acute myeloid leukemia (AML) patients. Despite these recent advances, their prognosis remains poor and new strategies to improve the standard anthracycline and Ara-C-based chemotherapy are needed. We recently published that dendrogenin A (DDA), a mammalian cholesterol ...
Published in Journal of proteome research
Severe acute pancreatitis (SAP) is caused by complicated biological factors, and revealing its complex pathogenesis by single-target analysis is difficult. Systematic studies have developed slowly because extraction of degradable pancreatic proteins exposed to multiple proteases is challenging. We present integrated whole proteomic and phosphoprote...
Delivered as part of the session "Unlocking the Value of the Monograph" at Electronic Resources and Libraries (ER&L) 15th Annual Conference, Austin, Texas, March 9, 2020. Scholarly monographs are undergoing a digital transformation that brings new value to both libraries and researchers. This presentation approaches the session theme with a single ...
Published in Metabolites
Constant improvements to the Orbitrap mass analyzer, such as acquisition speed, resolution, dynamic range and sensitivity have strengthened its value for the large-scale identification and quantification of metabolites in complex biological matrices. Here, we report the development and optimization of Data Dependent Acquisition (DDA) and Sequential...
Published in Journal of proteome research
Phosphorylation-driven cell signaling governs most biological functions and is widely studied using mass-spectrometry-based phosphoproteomics. Identifying the peptides and localizing the phosphorylation sites within them from the raw data is challenging and can be performed by several algorithms that return scores that are not directly comparable. ...
Published in Molecular & Cellular Proteomics : MCP
State-of-the-art proteomics-grade mass spectrometers can measure peptide precursors and their fragments with ppm mass accuracy at sequencing speeds of tens of peptides per second with attomolar sensitivity. Here we describe a compact and robust quadrupole-orbitrap mass spectrometer equipped with a front-end High Field Asymmetric Waveform Ion Mobili...
Published in Plants
Diatom–diazotroph associations (DDAs) are symbioses where trichome-forming cyanobacteria support the host diatom with fixed nitrogen through dinitrogen (N2) fixation. It is inferred that the growth of the trichomes is also supported by the host, but the support mechanism has not been fully quantified. Here, we develop a coarse-grained, cellular mod...
Di-nitrogen (N2) fixers, also called diazotrophs, are able to reduce atmospheric N2 into bioavailable nitrogen, giving them an advantage in open ocean regions with low dissolved inorganic nitrogen concentrations. The focus of this thesis are three lineages of symbiotic heterocystous filamentous types (het-1, het-2 and het-3), that associate with se...
A profound understanding of the scattering properties of hydrometeors is key to extract information from passive and active MW observations. The natural variety of hydrometeor properties also leads to the complexity of how they interact with electromagnetic radiation. The challenge of calculating hydrometeor scattering properties is not only the sc...
Published in Advances in therapy
The new direct acting antiviral (DAA) therapies are able to effectively treat chronic hepatitis C (CHC). This study elicited the preferences of CHC patients for treatment attributes of new DAAs. An online discrete choice experiment survey was designed to collect data from adult CHC patients in the USA, UK, France, Germany, Spain, and Italy. Patient...
