VerPlank, Jordan JS Tyrkalska, Sylwia Fleming, Angeleen Rubinsztein, David Goldberg, Alfred L
Because raising cAMP enhances 26S proteasome activity and the degradation of cell proteins, including the selective breakdown of misfolded proteins, we investigated whether agents that raise cGMP may also regulate protein degradation. Treating various cell lines with inhibitors of phosphodiesterase 5 or stimulators of soluble guanylyl cyclase rapid...
Chan, Yu-Tzu Lai, Alan C-Y Lin, Ruey-Jen Wang, Ya-Hui Wang, Yi-Ting Chang, Wen-Wei Wu, Hsin-Yi Lin, Yu-Ju Chang, Wen-Ying Wu, Jen-Chine
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G protein-coupled estrogen receptor-1 (GPER), a member of the G protein-coupled receptor (GPCR) superfamily, mediates estrogen-induced proliferation of normal and malignant breast epithelial cells. However, its role in breast cancer stem cells (BCSCs) remains unclear. Here we showed greater expression of GPER in BCSCs than non-BCSCs of three patien...
Chien, Jason Wolf, Fred Grosche, Sarah Yosef, Nebeyu GARRIGA, Gian Mörck, Catarina
Caenorhabditis elegans larval development requires the function of the two Canal-Associated Neurons (CANs): killing the CANs by laser microsurgery or disrupting their development by mutating the gene ceh-10 results in early larval arrest. How these cells promote larval development, however, remains a mystery. In screens for mutations that bypass CA...
Ohadi, Donya Rangamani, Padmini
The spatiotemporal regulation of cyclic adenosine monophosphate (cAMP) and its dynamic interactions with other second messengers such as calcium are critical features of signaling specificity required for neuronal development and connectivity. cAMP is known to contribute to long-term potentiation and memory formation by controlling the formation an...
Ohadi, Donya Schmitt, Danielle L Calabrese, Barbara Halpain, Shelley Zhang, Jin Rangamani, Padmini
Dendritic spines are the primary excitatory postsynaptic sites that act as subcompartments of signaling. Ca2+ is often the first and most rapid signal in spines. Downstream of calcium, the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway plays a critical role in the regulation of spine formation, morphological modifications, and...
Shen, Ao Chen, Dana Kaur, Manpreet Bartels, Peter Xu, Bing Shi, Qian Martinez, Joseph M Man, Kwun-Nok Mimi Nieves-Cintron, Madeline Hell, Johannes W
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G protein-coupled receptors (GPCRs) transduce pleiotropic intracellular signals in mammalian cells. Here, we report neuronal excitability of β-blockers carvedilol and alprenolol at clinically relevant nanomolar concentrations. Carvedilol and alprenolol activate β2AR, which promote G protein signaling and cAMP/PKA activities without action of G prot...
Prada, Maria Paz Syed, Arsalan U Buonarati, Olivia R Reddy, Gopireddy R Nystoriak, Matthew A Ghosh, Debapriya Simó, Sergi Sato, Daisuke Sasse, Kent C Ward, Sean M
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Elevated glucose increases vascular reactivity by promoting L-type CaV1.2 channel (LTCC) activity by protein kinase A (PKA). Yet, how glucose activates PKA is unknown. We hypothesized that a Gs-coupled P2Y receptor is an upstream activator of PKA mediating LTCC potentiation during diabetic hyperglycemia. Experiments in apyrase-treated cells suggest...
Song, Wenxin Luo, Qi Zhang, Yuping Zhou, Linkang Liu, Ye Ma, Zhilong Guo, Jianan Huang, Yuedong Cheng, Lili Meng, Ziyi
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Beiging of white adipose tissue (WAT) is a particularly appealing target for therapeutics in the treatment of metabolic diseases through norepinephrine (NE)-mediated signaling pathways. Although previous studies report NE clearance mechanisms via SLC6A2 on sympathetic neurons or proinflammatory macrophages in adipose tissues (ATs), the low catechol...
Suhr, Frank; 111316; Braun, Kristina; Vanmunster, Mathias; 110033; Bloch, Wilhelm;
BACKGROUND/AIMS: Calcium (Ca²⁺) coordinates skeletal muscle functions by controlling contractions as well as signaling pathways and transcriptional properties. The ryanodine receptor 1 (RyR1), its phosphorylation site (pRyR1Ser²⁸⁴⁰) and its stabilizers navigate Ca²⁺ oscillations to command muscle signaling cascades and transcriptional activities. W...
Ju, Won-Kyu Shim, Myoung Sup Kim, Keun-Young Park, Tae Lim Ahn, Sangphil Edwards, Genea Weinreb, Robert N
Glaucoma is characterized by a progressive optic nerve degeneration and retinal ganglion cell loss, but the underlying biological basis for the accompanying neurodegeneration is not known. Accumulating evidence indicates that structural and functional abnormalities of astrocytes within the optic nerve head (ONH) have a role in glaucomatous neurodeg...