Correia, Cláudia Christoffersson, Jonas Tejedor, Sandra El-Haou, Saïd Matadamas-Guzman, Meztli Nair, Syam Dönnes, Pierre Musa, Gentian Rohman, Mattias Sundqvist, Monika
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Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) constitute an appealing tool for drug discovery, disease modeling, and cardiotoxicity screening. However, their physiological immaturity, resembling CMs in the late fetal stage, limits their utility. Herein, we have developed a novel, scalable cell culture medium designed to enhance the ...
Correia, Cláudia Christoffersson, Jonas Tejedor, Sandra El-Haou, Saïd Matadamas-Guzman, Meztli Nair, Syam Dönnes, Pierre Musa, Gentian Rohman, Mattias Sundqvist, Monika
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Peer reviewed: True / Acknowledgements: The authors acknowledge support from the National Genomics Infrastructure in Genomics Production Stockholm funded by Science for Life Laboratory, the Knut and Alice Wallenberg Foundation, the Swedish Research Council, and the SNIC/Uppsala Multidisciplinary Center for Advanced Computational Science for assista...
Revista MVZ Córdoba Fonseca-López, Dania Rodriguez Molano, Carlos Eduardo Monroy-Fonseca, Lily
Published in
Revista Mvz Córdoba
Objective. Evaluate lacto-serum as a source of mesophils with lactic acid activity in complex culture medium with sago starch (Canna indica L.). Materials and methods. Three culture media were analyzed for mesophilic lacto-serum bacteria differentiated by the inclusion of sago starch (Canna indica L.) (0, 0.5 and 1%). Control treatments were refrig...
Haney, Michael Pálovics, Róbert Munson, Christy Long, Chris Johansson, Patrik Yip, Oscar Dong, Wentao Rawat, Eshaan Tsai, Andy Guldner, Ian
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Several genetic risk factors for Alzheimers disease implicate genes involved in lipid metabolism and many of these lipid genes are highly expressed in glial cells1. However, the relationship between lipid metabolism in glia and Alzheimers disease pathology remains poorly understood. Through single-nucleus RNA sequencing of brain tissue in Alzheimer...
Srinivasan, Aparajitha Chen-Xiao, Kevin Banerjee, Deepanwita Oka, Asun Pidatala, Venkataramana R Eudes, Aymerick Simmons, Blake A Eng, Thomas Mukhopadhyay, Aindrila
The industrial amino acid production workhorse, Corynebacterium glutamicum naturally produces low levels of 2,3,5,6-tetramethylpyrazine (TMP), a valuable flavor, fragrance, and commodity chemical. Here, we demonstrate TMP production (∼0.8 g L-1) in C. glutamicum type strain ATCC13032 via overexpression of acetolactate synthase and/or α-acetolactate...
Sciorio, Romualdo Rinaudo, Paolo
In the last four decades, the assisted reproductive technology (ART) field has witnessed advances, resulting in improving pregnancy rates and diminishing complications, in particular reduced incidence of multiple births. These improvements are secondary to advanced knowledge on embryonic physiology and metabolism, resulting in the ability to design...
Shu, Yi Upara, Chawin Ding, Qiong Zhu, Min Zeng, Erliang Banas, Jeffrey A Hong, Liu
Published in
Journal of periodontology
The oral commensal bacterial species Streptococcus gordonii has been reported to regulate the inflammation of oral epithelial cells stimulated by the periodontal pathogen Porphyromonas gingivalis. This study investigated the activities of S. gordonii metabolites in S. gordonii spent culture supernatants (Sg-SCS) on periodontal-related bacterial gro...
Cosenza, Zachary Astudillo, Raul Frazier, Peter I Baar, Keith Block, David E
Culture media used in industrial bioprocessing and the emerging field of cellular agriculture is difficult to optimize due to the lack of rigorous mathematical models of cell growth and culture conditions, as well as the complexity of the design space. Rapid growth assays are inaccurate yet convenient, while robust measures of cell number can be ti...
Short, Walker D Steen, Emily Kaul, Aditya Wang, Xinyi Olutoye, Oluyinka O Vangapandu, Hima V Templeman, Natalie Blum, Alexander J Moles, Chad M Narmoneva, Daria A
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Endothelial progenitor cells (EPCs) contribute to de novo angiogenesis, tissue regeneration, and remodeling. Interleukin 10 (IL-10), an anti-inflammatory cytokine that primarily signals via STAT3, has been shown to drive EPC recruitment to injured tissues. Our previous work demonstrated that overexpression of IL-10 in dermal wounds promotes regener...
Mei, Jiyang Vasan, Aditya Magaram, Uri Takemura, Kenjiro Chalasani, Sreekanth H Friend, James
Three-dimensional cell agglomerates are broadly useful in tissue engineering and drug testing. We report a well-free method to form large (1.4-mm) multicellular clusters using 100-MHz surface acoustic waves (SAW) without direct contact with the media or cells. A fluid couplant is used to transform the SAW into acoustic streaming in the cell-laden m...
