Methods in molecular biology (Clifton, N.J.)
Quiescence (also called "G0") is the state in which cells have exited the cell cycle but are capable to reenter as required. Though poorly understood, it represents one of the most prevalent cell states across all life. Many biologically important cell types reside in quiescence including mature hepatocytes, endothelial cells, and dormant adult ste...
Cell Communication and Signaling
BackgroundLymphotoxin β receptor (LTβR) is a member of tumor necrosis factor receptor (TNFR) superfamily which regulates the immune response. At the cellular level, upon ligand binding, the receptor activates the pro-inflammatory NF-κB and AP-1 pathways. Yet, the intracellular distribution of LTβR, the routes of its endocytosis and their connection...
Current opinion in cell biology
Understanding of the range and mechanisms of clathrin functions has developed exponentially since clathrin's discovery in 1975. Here, newly established molecular mechanisms that regulate clathrin activity and connect clathrin pathways to differentiation, disease and physiological processes such as glucose metabolism are reviewed. Diversity and comm...
Neurobiology of Disease
• LRRK2 interacts with components of the clathrin adaptor complex AP2. • LRRK2 KO animals exhibit a significant depletion of AP2 and Clathrin. • The LRRK2-R1441C pathogenic mutation reduces endocytosis in cellular models. • Clathrin-dependent endocytosis genes are associated with Parkinson's disease.
Cellular stimuli that increase diacylglycerol levels activate several protein kinase C (PKC) isoforms; however, prolonged stimulation depletes cells of PKCs. Ubiquitination is a critical cellular event that mediates the degradation of numerous proteins, including PKCs, but little is known of the molecular mechanisms involved in PKC ubiquitination. ...
Journal of Cell Science
Highlighted Article: Functionalized GFP-binding nanobodies (dongles) can extend the use of GFP-tagged proteins. Here, dongles that allow knocksideways are demonstrated, but they are limited by perturbation of the target protein caused by dongle binding alone.
Journal of neurochemistry
The endolysosomal system is increasingly recognized as the key pathway affected in Parkinson’s disease (PD). Clathrin-mediated synaptic endocytosis, an integral part of the neuronal endolysosomal system, is probably the main early target. Autophagy, another important endolysosomal pathway that aids in maintaining proteostasis and a healthy mitochon...
Silica nanoparticles (SiNPs) are one of the popular nanomaterials used in industrial manufacturing, synthesis, engineering, and medicine. Recently, mechanisms underlying toxicity of silica nanoparticles have been reported; however, their uptake mechanisms have still not fully understood. In this study, toxicity of SiNPs was investigated in the nema...
Biochemical and biophysical research communications
Receptor transactivation or crosstalk refers to instances in which the signaling of a given receptor is regulated by different classes of receptors. Functional crosstalk between α4β2 nicotinic acetylcholine receptor (nAChR) and D3 dopamine receptor (D3R) that belong to the family of ligand-gated ion channels and G protein-coupled receptors, respect...