Published in Public health genomics
This study was undertaken to examine how a layperson is likely to interpret genetic information delivered in a clinical setting. A novel survey was designed to engage the reader in a simulated discussion of heritability as it might relate to human disease. The survey took approximately 5 min to administer. 307 individuals of different backgrounds c...
Published in Journal of cell science
Errors in mitotic chromosome segregation can lead to DNA damage and aneuploidy, both hallmarks of cancer. To achieve synchronous error-free segregation, mitotic chromosomes must align at the metaphase plate with stable amphitelic attachments to microtubules emanating from opposing spindle poles. The Astrin-Kinastrin/SKAP complex, also containing DY...
Published in Gut Pathogens
The emergence and transmission of the mobile colistin resistance gene (mcr-1) threatened the extensive use of polymyxin antimicrobials. Accumulated evidence showed that the banning of colistin additive in livestock feed efficiently reduce mcr-1 prevalence, not only in animals but also in humans and environments. However, our previous study has reve...
Published in BMC Genomics
BackgroundBelonging to lineage I of Brassicaceae, Camelina sativa is formed by two hybridizations of three species (three sub-genomes). The three sub-genomes were diverged from a common ancestor, likely derived from lineage I (Ancestral Crucifer karyotype, ACK). The karyotype evolutionary trajectories of the C. sativa chromosomes are currently unkn...
Published in BMC Biotechnology
BackgroundGene doctoring is an efficient recombination-based genetic engineering approach to mutagenesis of the bacterial chromosome that combines the λ-Red recombination system with a suicide donor plasmid that is cleaved in vivo to generate linear DNA fragments suitable for recombination. The use of a suicide donor plasmid makes Gene Doctoring mo...
Published in Biophysical Reviews
The high-order structure of mitotic chromosomes remains to be fully elucidated. How nucleosomes compact at various structural levels into a condensed mitotic chromosome is unclear. Cryogenic preservation and imaging have been applied for over three decades, keeping biological structures close to the native in vivo state. Despite being extensively u...
Published in Machine Vision and Applications
Chromosome analysis is an important approach to detecting genetic diseases. However, the process of identifying chromosomes in metaphase images can be challenging and time-consuming. Therefore, it is important to use automatic methods for detecting chromosomes to aid diagnosis. This work proposes a study of deep learning approaches for classificati...
Published in Essays in biochemistry
Proper regulation of centromeric cohesion is required for faithful chromosome segregation that prevents chromosomal instability. Extensive studies have identified and established the conserved protein Shugoshin (Sgo1/2) as an essential protector for centromeric cohesion. In this review, we summarize the current understanding of how Shugoshin-1 (Sgo...
Published in Genomics
Centromeric satellite DNA (cen-satDNA) sequences of the Asian swamp eel (Monopterus albus) were characterized. Three GC-rich cen-satDNA sequences were detected as a 233 bp MALREP-A and a 293 bp MALREP-B localized to all chromosomes, and a 293 bp MALREP-C distributed on eight chromosome pairs. Sequence lengths of MALREP-B and MALREP-C were 60 bp lar...
Objectif: comparer le statut pronucléaire de zygotes issus d'ICSI présentant une anomalie du nombre de pronucléus à leur composition chromosomique établit par hybridation in situ par fluorescence (FISH), en utilisant des sondes spécifiques des chromosomes X, Y et 18. Matériels : analyse cytologique à l'aide de sondes fluorescentes pour les chromoso...
