Tyler, James Ralston, Corie Y Rad, Behzad
In vivoimaging of protein complexes is a powerful method for understanding the underlying biological function of these key biomolecules. Though the engineering of small, high affinity nanobodies have become more prevalent, the off-rates of these tags may result in incomplete or partial labeling of proteins in live cells. The SpyCatcher003 and SpyTa...
Wang, Jeffrey Squire, Henry Goh, Natalie Ni, Heyuan Lien, Edward Wong, Cerise González-Grandío, Eduardo Landry, Markita
Using a fluorescence complementation assay, Delivered Complementation in Planta (DCIP), we demonstrate cell-penetrating peptide-mediated cytosolic delivery of peptides and recombinant proteins in Nicotiana benthamiana. We show that DCIP enables quantitative measurement of protein delivery efficiency and enables functional screening of cell-penetrat...
Greene, Lloyd A Zhou, Qing Siegelin, Markus D Angelastro, James M
Developing novel therapeutics often follows three steps: target identification, design of strategies to suppress target activity and drug development to implement the strategies. In this review, we recount the evidence identifying the basic leucine zipper transcription factors ATF5, CEBPB, and CEBPD as targets for brain and other malignancies. We d...
Saha, Abhishek Mandal, Shaswati Arafiles, Jan Vincent V Gómez-González, Jacobo Hackenberger, Christian P R Brik, Ashraf
Published in
Angewandte Chemie (International ed. in English)
Modifying cyclic cell-penetrating deca-arginine (cR10) peptides with 4-(4-dimethylaminophenylazo)benzoic acid (DABCYL) improves the uptake efficiency of synthetic ubiquitin (Ub) cargoes into living cells. To probe the role of the DABCYL moiety, we performed time-lapse microscopy and fluorescence lifetime imaging microscopy (FLIM) of fluorescent DAB...
Wang, Siwen Li, Zhonghan Aispuro, Desiree Guevara, Nathan Van Valkenburgh, Juno Chen, Boxi Zhou, Xiaoyun McCarroll, Matthew N Ji, Fei Cong, Xu
...
Delivering cargo molecules across the plasma membrane is critical for biomedical research, and the need to develop molecularly well-defined tags that enable cargo transportation is ever-increasing. We report here a hydrophilic endocytosis-promoting peptide (EPP6) rich in hydroxyl groups with no positive charge. EPP6 can transport a wide array of sm...
Li, Jie Tuma, Jan Han, Hesong Kim, Hansol Wilson, Ross Lee, Hye Murthy, Niren
Protein-based therapeutics have the potential to treat a variety of diseases, however, safe and effective methods for delivering them into cells need to be developed before their clinical potential can be realized. Peptide fusions have great potential for improving intracellular delivery of proteins. However, very few peptides have been identified ...
Longoria-García, Samuel Sánchez-Domínguez, Celia Nohemi Gallardo-Blanco, Hugo Leonid Gallardo Blanco, Hugo Leonid
Published in
Oncology Letters
Cell-penetrating peptides (CPPs) are small peptides from natural sources or designed from other protein sequences that can penetrate cell membranes. This property has been used in biomedicine to add them to biomolecules to improve their capacity for cell internalization and as a guidance tool for specific cell types. CPPs have been shown to enhance...
Hupy, Matthew Pedler, Michelle Shieh, Biehuoy Wang, Dongyan Wang, Xiao-Jing Petrash, J
Cataracts, a clouding of the eye lens, are a leading cause of visual impairment and are responsible for one of the most commonly performed surgical procedures worldwide. Although generally safe and effective, cataract surgery can lead to a secondary lens abnormality due to transition of lens epithelial cells to a mesenchymal phenotype (EMT) and opa...
Asher, Anthony H Theg, Steven M
The mechanism and pore architecture of the Tat complex during transport of folded substrates remain a mystery, partly due to rapid dissociation after translocation. In contrast, the proteinaceous SecY pore is a persistent structure that needs only to undergo conformational shifts between "closed" and "opened" states when translocating unfolded subs...
Ferreira, Abigail Moreira, Sara Lapa, Rui Vale, Nuno
Published in
ADMET & DMPK
Cancer is one of the most alarming diseases due to its high mortality and still increasing incidence rate. Currently available treatments for this condition present several shortcomings and new options are continuously being developed and evaluated, aiming at increasing the overall treatment efficiency and reducing associated adverse side effects. ...