Liu, Ke Liu, Jiao Zou, Borong Li, Changfeng Zeh, Herbert J Kang, Rui Kroemer, Guido Huang, Jun Tang, Daolin
Published in
Cellular and molecular gastroenterology and hepatology
Pancreatitis is characterized by acinar cell death and persistent inflammation. Ferroptosis is a type of lipid peroxidation-dependent necrosis, which is negatively regulated by glutathione peroxidase 4. We studied how trypsin, a serine protease secreted by pancreatic acinar cells, affects the contribution of ferroptosis to triggering pancreatitis. ...
Linsley, Jeremy W Shah, Kevan Castello, Nicholas Chan, Michelle Haddad, Dominik Doric, Zak Wang, Shijie Leks, Wiktoria Mancini, Jay Oza, Viral
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Cell death is a critical process that occurs normally in health and disease. However, its study is limited due to available technologies that only detect very late stages in the process or specific death mechanisms. Here, we report the development of a family of fluorescent biosensors called genetically encoded death indicators (GEDIs). GEDIs speci...
Digby, Zsofi Tourlomousis, Panagiotis Rooney, James Boyle, Joseph P Bibo-Verdugo, Betsaida Pickering, Robert J Webster, Steven J Monie, Tom Hopkins, Lee J Kayagaki, Nobuhiko
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Zoonotic pathogens, such as COVID-19, reside in animal hosts before jumping species to infect humans. The Carnivora, like mink, carry many zoonoses, yet how diversity in host immune genes across species affect pathogen carriage is poorly understood. Here, we describe a progressive evolutionary downregulation of pathogen-sensing inflammasome pathway...
Ramachandran, Shaliny Ma, Tiffany S Griffin, Jon Ng, Natalie Foskolou, Iosifina Hwang, Ming-Shih Victori, Pedro Cheng, Wei-Chen Buffa, Francesca M Leszczynska, Katarzyna B
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Tumour hypoxia is associated with poor patient prognosis and therapy resistance. A unique transcriptional response is initiated by hypoxia which includes the rapid activation of numerous transcription factors in a background of reduced global transcription. Here, we show that the biological response to hypoxia includes the accumulation of R-loops a...
Ecker, Veronika Stumpf, Martina Brandmeier, Lisa Neumayer, Tanja Pfeuffer, Lisa Engleitner, Thomas Ringshausen, Ingo Nelson, Nina Jücker, Manfred Wanninger, Stefan
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Current therapeutic approaches for chronic lymphocytic leukemia (CLL) focus on the suppression of oncogenic kinase signaling. Here, we test the hypothesis that targeted hyperactivation of the phosphatidylinositol-3-phosphate/AKT (PI3K/AKT)-signaling pathway may be leveraged to trigger CLL cell death. Though counterintuitive, our data show that gene...
Ecker, Veronika Stumpf, Martina Brandmeier, Lisa Neumayer, Tanja Pfeuffer, Lisa Engleitner, Thomas Ringshausen, Ingo Nelson, Nina Jücker, Manfred Wanninger, Stefan
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Current therapeutic approaches for chronic lymphocytic leukemia (CLL) focus on the suppression of oncogenic kinase signaling. Here, we test the hypothesis that targeted hyperactivation of the phosphatidylinositol-3-phosphate/AKT (PI3K/AKT)-signaling pathway may be leveraged to trigger CLL cell death. Though counterintuitive, our data show that gene...
Morris, Jordan L Gillet, Germain Prudent, Julien Popgeorgiev, Nikolay
Bcl-2 family proteins are considered as one of the major regulators of apoptosis. Indeed, this family is known to control the mitochondrial outer membrane permeabilization (MOMP): a central step in the mitochondrial pathway of apoptosis. However, in recent years Bcl-2 family members began to emerge as a new class of intracellular calcium (Ca2+) reg...
Hesse, Friederike Somai, Vencel Kreis, Felix Bulat, Flaviu Wright, Alan Brindle, Kevin
2H magnetic resonance spectroscopic imaging has been shown recently to be a viable technique for metabolic imaging in the clinic. We show here that 2H MR spectroscopy and spectroscopic imaging measurements of [2,3-2H2]malate production from [2,3-2H2]fumarate can be used to detect tumor cell death in vivo via the production of labeled malate. Produc...
Askarian, Fatemeh Uchiyama, Satoshi Masson, Helen Sørensen, Henrik Vinther Golten, Ole Bunæs, Anne Cathrine Mekasha, Sophanit Røhr, Åsmund Kjendseth Kommedal, Eirik Ludviksen, Judith Anita
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The recently discovered lytic polysaccharide monooxygenases (LPMOs), which cleave polysaccharides by oxidation, have been associated with bacterial virulence, but supporting functional data is scarce. Here we show that CbpD, the LPMO of Pseudomonas aeruginosa, is a chitin-oxidizing virulence factor that promotes survival of the bacterium in human b...
Chuang, Hsiu-Chun Nichols, Eva K Rauch, Isabella Chang, Wei-Cheng Lin, Patrick M Misra, Rhea Kitaoka, Maiko Vance, Russell E Saijo, Kaoru
Microglial cells are known to contribute to brain development and behaviors, but the mechanisms behind such functions are not fully understood. Here, we show that mice deficient in inflammasome regulators, including caspase-1 (Casp1), NLR family pyrin domain containing 3 (Nlrp3), IL-1 receptor (Il-1r), and gasdermin D (Gsdmd), exhibit behavior abno...
