Colomé, Núria Abian, Joaquín Aloria, Kerman Arizmendi, Jesús M Barceló-Batllori, Silvia Braga-Lagache, Sophie Burlet-Schiltz, Odile Carrascal, Montse Casal, J Ignacio Chicano-Gálvez, Eduard
...
Published in
Journal of proteomics
Global analysis of protein phosphorylation by mass spectrometry proteomic techniques has emerged in the last decades as a powerful tool in biological and biomedical research. However, there are several factors that make the global study of the phosphoproteome more challenging than measuring non-modified proteins. The low stoichiometry of the phosph...
Singh, Harshita Singh, Zeenu Zhu, Tingting Xu, Xiangyu Waghmode, Bhairavnath Garg, Tushar Yadav, Shivani Sircar, Debabrata De Smet, Ive Yadav, Shri Ram
...
Abstract
The rice root system is primarily composed of shoot-borne adventitious/crown roots (ARs/CRs) that develop from the coleoptile base, and therefore, it is an excellent model system for studying shoot-to-root trans-differentiation process. We reveal global changes in protein and metabolite abundance and protein phosphorylation in response to a...
Malyshev, Dmitry Öberg, Rasmus Dahlberg, Tobias Wiklund, Krister Landström, Lars Andersson, Per Ola Andersson, Magnus
Micro-Raman spectroscopy combined with optical tweezers is a powerful method to analyze how the biochemical composition and molecular structures of individual biological objects change with time. In this work we investigate laser induced effects in the trapped object. Bacillus thuringiensis spores, which are robust organisms known for their resilie...
Struk, Sylwia Braem, Lukas Matthys, Cedrick Walton, Alan Vangheluwe, Nick Van Praet, Stan Jiang, Lingxiang Baster, Pawel De Cuyper, Carolien Boyer, François-Didier
...
Published in
Plant & cell physiology
The synthetic strigolactone (SL) analog, rac-GR24, has been instrumental in studying the role of SLs as well as karrikins because it activates the receptors DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2) of their signaling pathways, respectively. Treatment with rac-GR24 modifies the root architecture at different levels, such as decreasing the lat...
chuanying, li cheng, rong lin, li chen, miaomiao cheng, wu
Duodenal ulcer seriously affects the quality of life and life safety of children, but the pathogenesis of children with duodenal ulcer is still unclear. As an important second messenger in the body, Ca2+ participates in the physiological and pathological processes of various diseases. Therefore, transient receptor potential vanilloid type 4 (TRPV4)...
Han, Chao Hua, Wenbo Li, Jinge Qiao, Yan Yao, Lianmei Hao, Wei Li, Ruizhi Fan, Min De Jaeger, Geert Yang, Wenqiang
...
Abstract
Starch is the main energy storage carbohydrate in plants and serves as an essential carbon storage molecule for plant metabolism and growth under changing environmental conditions. The TARGET of RAPAMYCIN (TOR) kinase is an evolutionarily conserved master regulator that integrates energy, nutrient, hormone, and stress signaling to regulate ...
Geeraerts, Xenia; Fernandez-Garcia, Juan; Hartmann, Felix J; de Goede, Kyra E; Martens, Liesbet; Elkrim, Yvon; Debraekeleer, Ayla; Stijlemans, Benoit; Vandekeere, Anke; 141389; Rinaldi, Gianmarco;
...
Macrophages are often prominently present in the tumor microenvironment, where distinct macrophage populations can differentially affect tumor progression. Although metabolism influences macrophage function, studies on the metabolic characteristics of ex vivo tumor-associated macrophage (TAM) subsets are rather limited. Using transcriptomic and met...
Wong, Andrew Kam Ho Kulpa, Deanna A Silvestri, Guido Maier, Cheryl L
Published in
STAR protocols
Here, we describe a protocol for cell-based detection of autoantibodies from human plasma and serum samples using a standard flow cytometer. The protocol allows detection of autoantibodies against a wide array of extracellular antigens. Antigen coverage is limited to the cell types tested, and researchers will need to further determine if autoantib...
Wurster, Sebastian Ruiz, Oscar E Tatara, Alexander M Kontoyiannis, Dimitrios P Eisenhoffer, George T
Published in
STAR protocols
Epithelia provide the first line of defense against foreign pathogens, and disruption of tissue homeostasis frequently allows for opportunistic infections. Here we provide a protocol for induction of epithelial cell loss in zebrafish larvae, followed by infection with fungal pathogens. Details are provided for monitoring larval survival after infec...
Io, Shingo Iemura, Yoshiki Takashima, Yasuhiro
Published in
STAR protocols
Human trophoblasts arise from the morula as trophectoderm, which differentiates into cytotrophoblast, syncytiotrophoblast, and extravillous trophoblast after implantation. Here, we present a robust step-by-step protocol to induce trophectoderm (TE) from naive human pluripotent stem cells (PSCs) corresponding to pre-implantation epiblast. Our cultur...
