Farhat, Sarah Le, Phuong Kayal, Ehsan Noel, Benjamin Bigeard, Estelle Corre, Erwan Maumus, Florian Florent, Isabelle Alberti, Adriana Aury, Jean-Marc
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Published in
BMC Biology
BackgroundDinoflagellates are aquatic protists particularly widespread in the oceans worldwide. Some are responsible for toxic blooms while others live in symbiotic relationships, either as mutualistic symbionts in corals or as parasites infecting other protists and animals. Dinoflagellates harbor atypically large genomes (~ 3 to 250 Gb), with gene...
Kabelac, Zachary Tarolli, Christopher G. Snyder, Christopher Feldman, Blake Glidden, Alistair Hsu, Chen-Yu Hristov, Rumen Dorsey, E. Ray Katabi, Dina
Published in
Digital Biomarkers
We conducted a pilot study using a passive radio-wave-based home monitor in individuals with Parkinson disease (PD) with a focus on gait, home activity, and time in bed. We enrolled 7 ambulatory individuals to have the device installed in the bedroom of their homes over 8 weeks and performed standard PD assessments at baseline. We evaluated the abi...
Beerts, Charlotte Brondeel, Carlien Pauwelyn, Glenn Depuydt, Eva Tack, Liesa Duchateau, Luc Xu, Yangfeng Saunders, Jimmy H. Peremans, Kathelijne Spaas, Jan H.
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Published in
Stem Cell Research & Therapy
BackgroundMesenchymal stem cell treatments in dogs have been investigated as a potential innovative alternative to current conventional therapies for a variety of conditions. So far, the precise mode of action of the MSCs has yet to be determined. The aim of this study was to gain more insights into the pharmacokinetics of MSCs by evaluating their ...
Hou, Yuqing Zhao, Lei Kubo, Tomohiro Cheng, Xi McNeill, Nathan A. Oda, Toshiyuki Witman, George B.
Cilia are essential organelles required for cell signaling and motility. Nearly all motile cilia have a '9+2' axoneme composed of nine outer doublet microtubules plus two central microtubules; the central microtubules together with their projections are termed the central apparatus (CA). In Chlamydomonas reinhardtii, a model organism for studying c...
Ermine, Charlotte M. Chavez, Carolina A. Thompson, Lachlan H.
Published in
STAR Protocols
This protocol describes the identification and characterization of newborn cells generated in the rodent brain after injury through birthdating with the thymidine analog 5-bromo-2′-deoxyuridine, followed by immunohistochemical labeling of fixed tissue sections. We also describe a software-assisted approach for automated detection and quantification...
Wang, Lihui Wang, Zhiguo Liu, Jun-Ping
Published in
STAR Protocols
Telomere dysfunction-induced focus (TIF) assay allows efficient profiling of telomere dysfunctions in cells and tissues. Here, we describe the use of the TIF assay to screen synthetic peptides from E3 ubiquitin ligase FBW7, a tumor suppressor gene product, to prevent TIFs caused by environmental radiation stress. We demonstrate peptidomimetic telom...
Yang, Yun Zhang, Guimin Yang, Tao Gan, Jia Xu, Lin Yang, Hanshuo
Published in
STAR protocols
Hypoxia is known to stimulate mitochondrial reactive oxygen species (mROS) in cells. Here, we present a detailed protocol to detect mROS using MitoSOX staining in live cells under normoxia and hypoxia. Flow cytometry allows sensitive and reliable quantification of mROS by FlowJo software. We optimized several aspects of the procedure including hypo...
Liu, Lu Besson-Girard, Simon Ji, Hao Gehring, Katrin Bulut, Buket Kaya, Tuğberk Usifo, Fumere Simons, Mikael Gokce, Ozgun
Published in
STAR protocols
Single-cell RNA sequencing (scRNA-seq) provides the transcriptome of individual cells and addresses previously intractable problems including the central nervous system's transcriptional responses during health and disease. However, dissociating brain cells is challenging and induces artificial transcriptional responses. Here, we describe an enzyma...
Zellner, Susanne Nalbach, Karsten Behrends, Christian
Published in
STAR protocols
The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry, suitable to specifically map the content of autophagosomes and potentially other short-lived endomembrane tr...
Fra-Bido, Sigrid Walker, Simon A. Innocentin, Silvia Linterman, Michelle A.
Published in
STAR Protocols
Location of immune cells that form the germinal center reaction within secondary lymphoid tissues can be characterized using confocal microscopy. Here, we present an optimized immunofluorescence staining protocol to image germinal center structures in fixed/frozen spleen sections from ChAdOx1 nCoV-19 immunized mice. This protocol can be adapted to ...
