Xie, Linlin Chen, Lang Zhong, Chaojie Yu, Ting Ju, Zhao Wang, Meirong Xiong, Hairong Zeng, Yan Wang, Jianhua Hu, Haitao
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Published in
Retrovirology
BackgroundThe human myxovirus resistance 2 (Mx2/MxB) protein was originally found to regulate cytoplasmic-nuclear transport but was recently reported to restrict HIV-1 replication by binding to HIV-1 capsid (CA), preventing uncoating, the nuclear import of pre-integration complex (PIC) and viral DNA integration. This work explores the mechanisms of...
Freesmeyer, Martin Winkens, Thomas Weissenrieder, Luis Kühnel, Christian Gühne, Falk Schenke, Simone Drescher, Robert Seifert, Philipp
Published in
Sensors (Basel, Switzerland)
In conventional thyroid diagnostics, the topographical correlation between thyroid nodules (TN) depicted on ultrasound (US) in axial or sagittal orientation and coronally displayed scintigraphy images can be challenging. Sensor-navigated I-124-PET/US fusion imaging has been introduced as a problem-solving tool for ambiguous cases. The purpose of th...
Saito, Akatsuki Sultana, Tahmina Ode, Hirotaka Nohata, Kyotaro Samune, Yoshihiro Nakayama, Emi E Iwatani, Yasumasa Shioda, Tatsuo
Published in
AIDS research and human retroviruses
Binding of HIV-1 capsid (CA) to cleavage and polyadenylation specificity factor 6 (CPSF6) is hypothesized to provide a significant fitness advantage to in vivo viral replication, explaining why CA-CPSF6 interactions are strictly conserved in primate lentiviruses. We recently identified a Q4R mutation in CA after propagation of an interferon (IFN)-β...
Wang, Bi-Jun Liu, Da-Chao Guo, Qian-Ying Han, Xiao-Wen Bi, Xiao-Min Wang, Hao Wu, Zheng-Sheng Wu, Wen-Yong
Published in
Cancer management and research
NUDT21, an RNA binding protein, has been reported to play an important role in the regulation of multiple biological responses. Detection of NUDT21 expression may lead to the identification of a novel marker for breast cancer. The aim of this study was to investigate the clinical significance and functional role of NUDT21 in breast cancer. The prot...
Wang, Xiaomei Xu, Peng Cheng, Fang Li, Yi Wang, Zekun Hao, Siyuan Wang, Jianke Ning, Kang Ganaie, Safder S Engelhardt, John F
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Published in
Journal of virology
Human bocavirus 1 (HBoV1), which belongs to the genus Bocaparvovirus of the Parvoviridae family, causes acute respiratory tract infections in young children. In vitro, HBoV1 infects polarized primary human airway epithelium (HAE) cultured at an air-liquid interface (HAE-ALI). HBoV1 encodes a small nonstructural protein, nuclear protein 1 (NP1), tha...
Zila, Vojtech Müller, Thorsten G Laketa, Vibor Müller, Barbara Kräusslich, Hans-Georg
Published in
mBio
HIV-1 infects host cells by fusion at the plasma membrane, leading to cytoplasmic entry of the viral capsid encasing the genome and replication machinery. The capsid eventually needs to disassemble, but time and location of uncoating are not fully characterized and may vary depending on the host cell. To study the fate of the capsid by fluorescence...
Saito, Akatsuki Ode, Hirotaka Nohata, Kyotaro Ohmori, Hisaki Nakayama, Emi E Iwatani, Yasumasa Shioda, Tatsuo
Published in
Virology
The HIV-1 capsid (CA) utilizes CPSF6 for nuclear entry and integration site targeting. Previous studies demonstrated that the HIV-1 CA C-terminal domain (CTD) contains a highly conserved K182 residue involved in interaction with CPSF6. In contrast, certain HIV-2 strains possess a substitution at this residue (K182R). To assess whether CA-CPSF6 inte...
Achuthan, Vasudevan Perreira, Jill M Ahn, Jenny J Brass, Abraham L Engelman, Alan N
Published in
Journal of life sciences (Westlake Village, Calif.)
HIV-1 integration favors active chromatin, which is primarily mediated through interactions between the viral capsid and integrase proteins with host factors cleavage and polyadenylation specificity factor 6 (CPSF6) and lens epithelium-derived growth factor/p75, respectively. Previously published image-based studies had suggested that HIV-1 prefers...
Zuliani-Alvarez, Lorena Towers, Greg J
Published in
eLife
Identification of a protein that pulls HIV into the nucleus explains a key step in HIV infection. © 2019, Zuliani-Alvarez and Towers.
Bejarano, David Alejandro Peng, Ke Laketa, Vibor Börner, Kathleen Jost, K Laurence Lucic, Bojana Glass, Bärbel Lusic, Marina Müller, Barbara Kräusslich, Hans-Georg
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Published in
eLife
Nuclear entry of HIV-1 replication complexes through intact nuclear pore complexes is critical for successful infection. The host protein cleavage-and-polyadenylation-specificity-factor-6 (CPSF6) has been implicated in different stages of early HIV-1 replication. Applying quantitative microscopy of HIV-1 reverse-transcription and pre-integration-co...
