Crohn’s disease is a waxing and waning lifelong inflammatory condition that affects the colon (large bowel) and small bowel. Treatment relies on accurately determining disease extent and underlying inflammation. Colonoscopy is very good for examining the colon, but it is invasive and, at best, can only visualise a few centimetres of the small bowel...
Raf kinase inhibitor protein (RKIP) appears to control cancer cell metastasis and its expression in colonic tissue is related to colonic cancer development. We sought to identify the roles of RKIP in maintaining homeostasis of GI tract. The expression of RKIP was determined by immunohistochemistry and western blot analysis. RKIP knockout and wild-t...
Law, Ivy Ka ManBakirtzi, KyriakiPolytarchou, ChristosOikonomopoulos, AngelosHommes, DanielIliopoulos, DimitriosPothoulakis, Charalabos
Neurotensin (NT) mediates colonic inflammation through its receptor neurotensin receptor 1 (NTR1). NT stimulates miR-133α expression in colonic epithelial cells. We investigated the role of miR-133α in NT-associated colonic inflammation in vitro and in vivo.miR-133α and aftiphilin (AFTPH) levels were measured by quantitative PCR. Antisense (as)-miR...
Neurotensin (NT) mediates colonic inflammation through its receptor neurotensin receptor 1 (NTR1). NT stimulates miR-133α expression in colonic epithelial cells. We investigated the role of miR-133α in NT-associated colonic inflammation in vitro and in vivo. miR-133α and aftiphilin (AFTPH) levels were measured by quantitative PCR. Antisense (as)-mi...
Clostridium difficile is a major cause of nosocomial infectious diarrhoea. Treatment of C. difficile infection (CDI) depends on disease severity. A combination of vancomycin and metronidazole is often recommended in severe cases. The aim of this study was to examine, in a murine model of CDI, if mice treated with a combination of vancomycin and met...
