Synthesis of uracil-coumarin conjugates as potential inhibitors of virus replication
status: published
Assay optimisation and age-related baseline variation in biochemical markers in Lesser Black-backed gulls.
Published in Ecotoxicology and environmental safety
Free-ranging animals are often used as bioindicators of both short- and long-term changes in ecosystem health, mainly to detect the presence and effects of contaminants. Birds, and gulls in particular, have been used as bioindicators over a broad range of marine and terrestrial ecosystems. In this study, we standardise the conditions for the use of...
Dosage en microplaque de l’activité enzymatique AChE du gammare
Cette méthode décrit la mesure de l’activité enzymatique AChE (Acétylcholinestérase) par colorimétrie sur microplaque 96 puits à partir de la fraction post-mitochondriale (S9) d’un homogénat de gammare. Cette mesure peut être réalisée avec des homogénats gammares pêchés sur le terrain ou exposés en laboratoire. Elle permet le diagnostic de l’exposi...
Laboratory measurements of biomarkers and individual performances in Chironomus xanthus to evaluate pesticide contaminat...
Research Support Foundation of the State of Sao Paulo (FAPESP)
Laboratory measurements of biomarkers and individual performances in Chironomus xanthus to evaluate pesticide contaminat...
Research Support Foundation of the State of Sao Paulo (FAPESP)
Laboratory measurements of biomarkers and individual performances in Chironomus xanthus to evaluate pesticide contaminat...
Research Support Foundation of the State of Sao Paulo (FAPESP)
B-type esterases in the snail Xeropicta derbentina: an enzymological analysis to evaluate their use as biomarkers of pes...
The study was prompted to characterize the B-type esterase activities in the terrestrial snail Xeropicta derbentina and to evaluate its sensitivity to organophosphorus and carbamate pesticides. Specific cholinesterase and carboxylesterase activities were mainly obtained with acetylthiocholine (Km =77.2 mM; Vmax= 38.2 mU/mg protein) and 1-naphthyl a...
In vitro expression, kinetic, pharmacologic, molecular, and evolutionary analyses, and molecular modeling of a recombina...
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To learn more about the evolution of the cholinesterases, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) in the vertebrates, we investigated the AChE activity of a deuterostome invertebrate, the urochordate Ciona intestinalis, by expressing in vitro a synthetic recombinant cDNA for the enzyme in COS-7 cells. On the basis of evidence ...
Tissue distribution and characterization of cholinesterase activity in six earthworm species
To validate cholinesterase activity as a biomarker of pesticide exposure, we characterized the tissue distribution (whole body, nervous tissue and crop/gizzard), activity at two seasons of cholinesterase in six different species of earthworms collected in an unpolluted field: Lumbricus terrestris, Lumbricus castaneus, Aporrectodea nocturna, Aporrec...
Cholinesterase activity as a biomarker of pesticide exposure in Allolobophora chlorotica earthworms living in apple orch...
The present study used cholinesterase (ChE) activity in earthworms as a biomarker of pesticide exposure at 17 apple orchards using different pest protection strategies (organic, integrated pest management [IPM], conventional, and abandoned) located within a 300-km2 subregion near Avignon in southeastern France). The most common earthworm species in...
