Phi29 DNA polymerase (DNAP) derives from bacteriophage Phi29 and replicates DNA under isothermal conditions by rolling circle amplification. It is a particularly interesting enzyme due to its outstanding processivity and low error rates in the range of one base-pair mismatch for every 10^(-5) to 10^(-6) nucleotides incorporated. Phi29 DNAP achieves...
Phi29 DNA polymerase (DNAP) derives from bacteriophage Phi29 and replicates DNA under isothermal conditions by rolling circle amplification. It is a particularly interesting enzyme due to its outstanding processivity and low error rates in the range of one base-pair mismatch for every 10^(-5) to 10^(-6) nucleotides incorporated. Phi29 DNAP achieves...
Au-delà des structures duplex, il existe des preuves convaincantes qui suggèrent que les acides nucléiques peuvent former des structures secondaires telles que la structure G-quadruplex (G4). Le G-quadruplex est formé par des interactions non canoniques de nucléobases au sein de séquences d'ADN et d'ARN riches en G. Ces structures, formées dans des...
The clinical presentation of plasma cell disorders is heterogeneous ranging from asymptomatic conditions such as Monoclonal Gammopathy of Undetermined Significance (MGUS) or Smouldering Multiple Myeloma (SMM) to symptomatic form of diseases such as Multiple Myeloma (MM) or light chain Amyloidosis (AL). This work aims at understanding the genetic ba...
Published in Biochemistry (Moscow)
AbstractIn response to stress, eubacteria reduce the level of protein synthesis and either disassemble ribosomes into the 30S and 50S subunits or turn them into translationally inactive 70S and 100S complexes. This helps the cell to solve two principal tasks: (i) to reduce the cost of protein biosynthesis under unfavorable conditions, and (ii) to p...
Published in Scientific Reports
Parthenocarpy, or pollination-independent fruit set, is an attractive trait for fruit production and can be induced by increased responses to the phytohormone gibberellin (GA), which regulates diverse aspects of plant development. GA signaling in plants is negatively regulated by DELLA proteins. A loss-of-function mutant of tomato DELLA (SlDELLA), ...
L'agronomie est un domaine important constitué de divers domaines de recherche tels que la génétique, la biologie moléculaire des plantes, l'écologie et les sciences de la terre. Les dernières décennies ont vu le succès du développement de technologies à haut débit qui ont révolutionnés et transformés la recherche agronomique. L'application de ces ...
