Glasgow, Anum Glasgow, Jeff Limonta, Daniel Solomon, Paige Lui, Irene Zhang, Yang Nix, Matthew A Rettko, Nicholas J Zha, Shoshana Yamin, Rachel
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An essential mechanism for severe acute respiratory syndrome coronavirus 1 (SARS-CoV-1) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection begins with the viral spike protein binding to the human receptor protein angiotensin-converting enzyme II (ACE2). Here, we describe a stepwise engineering approach to generate a set of a...
Clausen, Thomas Mandel Sandoval, Daniel R Spliid, Charlotte B Pihl, Jessica Perrett, Hailee R Painter, Chelsea D Narayanan, Anoop Majowicz, Sydney A Kwong, Elizabeth M McVicar, Rachael N
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We show that SARS-CoV-2 spike protein interacts with both cellular heparan sulfate and angiotensin-converting enzyme 2 (ACE2) through its receptor-binding domain (RBD). Docking studies suggest a heparin/heparan sulfate-binding site adjacent to the ACE2-binding site. Both ACE2 and heparin can bind independently to spike protein in vitro, and a terna...
Bridges, Hannah Fedor, Justin G Blaza, James N Di Luca, Andrea Jussupow, Alexander Jarman, Owen D Wright, John J Agip, Ahmed-Noor A Gamiz-Hernandez, Ana P Roessler, Maxie M
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Park, SeHee Doherty, Erin E Xie, Yixuan Padyana, Anil K Fang, Fang Zhang, Yue Karki, Agya Lebrilla, Carlito B Siegel, Justin B Beal, Peter A
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Adenosine Deaminases that act on RNA (ADARs) are enzymes that catalyze adenosine to inosine conversion in dsRNA, a common form of RNA editing. Mutations in the human ADAR1 gene are known to cause disease and recent studies have identified ADAR1 as a potential therapeutic target for a subset of cancers. However, efforts to define the mechanistic eff...
Douse, Christopher H Tchasovnikarova, Iva A Timms, Richard T Protasio, Anna V Seczynska, Marta Prigozhin, Daniil M Albecka, Anna Wagstaff, Jane Williamson, James C Freund, Stefan MV
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The HUSH complex represses retroviruses, transposons and genes to maintain the integrity of vertebrate genomes. HUSH regulates deposition of the epigenetic mark H3K9me3, but how its three core subunits - TASOR, MPP8 and Periphilin - contribute to assembly and targeting of the complex remains unknown. Here, we define the biochemical basis of HUSH as...
Byrnes, James R Zhou, Xin X Lui, Irene Elledge, Susanna K Glasgow, Jeff E Lim, Shion A Loudermilk, Rita P Chiu, Charles Y Wang, Taia T Wilson, Michael R
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As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread around the world, there is an urgent need for new assay formats to characterize the humoral response to infection. Here, we present an efficient, competitive serological assay that can simultaneously determine an individual's seroreactivity against the SARS-CoV-2 Sp...
Douse, Christopher H Tchasovnikarova, Iva Timms, Richard Protasio, Anna V Seczynska, Marta Prigozhin, Daniil M Albecka, Anna Wagstaff, Jane Williamson, James Freund, Stefan MV
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The HUSH complex represses retroviruses, transposons and genes to maintain the integrity of vertebrate genomes. HUSH regulates deposition of the epigenetic mark H3K9me3, but how its three core subunits – TASOR, MPP8 and Periphilin – contribute to assembly and targeting of the complex remains unknown. Here we define the biochemical basis of HUSH ass...
Damas, Joana Hughes, Graham M Keough, Kathleen C Painter, Corrie A Persky, Nicole S Corbo, Marco Hiller, Michael Koepfli, Klaus-Peter Pfenning, Andreas R Zhao, Huabin
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The novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of COVID-19. The main receptor of SARS-CoV-2, angiotensin I converting enzyme 2 (ACE2), is now undergoing extensive scrutiny to understand the routes of transmission and sensitivity in different species. Here, we utilized a unique dataset of ACE2 sequenc...
Thomas, Sherine Whitehouse, Andrew J Brown, Karen Burbaud, Sophie Belardinelli, Juan M Sangen, Jasper Lahiri, Ramanuj Libardo, Mark Daben J Gupta, Pooja Malhotra, Sony
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Rogers, Thomas F Zhao, Fangzhu Huang, Deli Beutler, Nathan Burns, Alison He, Wan-Ting Limbo, Oliver Smith, Chloe Song, Ge Woehl, Jordan
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Countermeasures to prevent and treat coronavirus disease 2019 (COVID-19) are a global health priority. We enrolled a cohort of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-recovered participants, developed neutralization assays to investigate antibody responses, adapted our high-throughput antibody generation pipeline to rapidly scr...