Muconic acid isomers as platform chemicals and monomers in the biobased economy
status: published
From second generation feed-stocks to innovative fermentation and downstream techniques for succinic acid production
Succinic acid (SA) is one of the most important biobuilding blocks in biorefinery. Its production from fermentation of renewable biomass sources is becoming a consolidated alternative that is more sustainable and potentially more economic than the traditional petroleum-based path for SA production. Fermentative production of SA has been successfull...
From second generation feed-stocks to innovative fermentation and downstream techniques for succinic acid production
Succinic acid (SA) is one of the most important biobuilding blocks in biorefinery. Its production from fermentation of renewable biomass sources is becoming a consolidated alternative that is more sustainable and potentially more economic than the traditional petroleum-based path for SA production. Fermentative production of SA has been successfull...
Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase
...
Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer compounds, several aspects of th...
Pathway optimization by re-design of untranslated regions for L-tyrosine production in Escherichia coli
L-tyrosine is a commercially important compound in the food, pharmaceutical, chemical, and cosmetic industries. Although several attempts have been made to improve L-tyrosine production, translation-level expression control and carbon flux rebalancing around phosphoenolpyruvate (PEP) node still remain to be achieved for optimizing the pathway. Here...
Xylosylation as an effective means for reducing yeast growth inhibition by 2-phenylethanol.
Published in Journal of basic microbiology
Microbial bioproduction processes of 2-phenylethanol, an important rose-like flavor and fragrance compound that occurs naturally in the essential oils of many flowers and plants, are hindered by the growth inhibition it exerts towards the producing microorganism, mainly yeast. We show here for the first time that glycosylation of 2-phenylethanol wi...
Integrating the protein and metabolic engineering toolkits for next-generation chemical biosynthesis.
Published in ACS chemical biology
Through microbial engineering, biosynthesis has the potential to produce thousands of chemicals used in everyday life. Metabolic engineering and synthetic biology are fields driven by the manipulation of genes, genetic regulatory systems, and enzymatic pathways for developing highly productive microbial strains. Fundamentally, it is the biochemical...
Efficient production and characterization of the sweet-tasting brazzein secreted by the yeast Pichia pastoris.
Published in Journal of agricultural and food chemistry
Brazzein is a small, heat-, and pH-stable sweet protein present in the fruits of the West African plant Pentadiplandra brazzeana Baillon. It exists in two forms differing in sweetness intensity. The major form, called pyrE-bra, contains a pyroglutamic acid at its N-terminus, while the minor form, called des-pyrE-bra, lacks this residue. Here we des...
Screening, immobilization and utilization of whole cell biocatalysts to mediate the ethanolysis of babassu oil
The screening. biomass growth of lipase-producing fungus isolated from different sources and available at URM (University Recife Mycologia). as well as, the immobilization and utilization of the whole cells for the transesterification of babassu oil were investigated. Rhizopus oryzae (URM 3231, 4692), Mucor circinelloides (URM 4140, 4182) and Penic...
Screening, immobilization and utilization of whole cell biocatalysts to mediate the ethanolysis of babassu oil
The screening. biomass growth of lipase-producing fungus isolated from different sources and available at URM (University Recife Mycologia). as well as, the immobilization and utilization of the whole cells for the transesterification of babassu oil were investigated. Rhizopus oryzae (URM 3231, 4692), Mucor circinelloides (URM 4140, 4182) and Penic...
