Diago-Sempere, Elena Bueno, José Luis Sancho-López, Aránzazu Rubio, Elena Múñez Torres, Ferrán de Molina, Rosa Malo Fernández-Cruz, Ana de Diego, Isabel Salcedo Velasco-Iglesias, Ana Payares-Herrera, Concepción
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Published in
Trials
Background COVID-19 is a respiratory disease caused by a novel coronavirus (SARS-CoV-2) and causes substantial morbidity and mortality. At the time this clinical trial was planned, there were no available vaccine or therapeutic agents with proven efficacy, but the severity of the condition prompted the use of several pharmacological and non-pharmac...
Kim, Cheolmin Ryu, Dong-Kyun Lee, Jihun Kim, Young-Il Seo, Ji-Min Kim, Yeon-Gil Jeong, Jae-Hee Kim, Minsoo Kim, Jong-In Kim, Pankyeom
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Published in
Nature Communications
Therapies and vaccines for COVID-19, caused by the SARS-CoV-2 viral pandemic, are urgently needed. Here the authors establish and screen an antibody library from a convalescent COVID-19 patient to isolate a neutralizing antibody with the ability to reduce viral titer and alleviate symptoms in ferret, hamster, and rhesus monkey infection models.
Rochereau, Nicolas Roblin, Xavier Michaud, Eva Gayet, Rémi Chanut, Blandine Jospin, Fabienne Corthésy, Blaise Paul, Stéphane
Published in
Nature Communications
Trafficking of IgA/commensal complex in the gut has been implicated in inflammatory bowel diseases such as Crohn’s disease, but molecular insights are still lacking. Here the authors show, using mouse model or human cells, that NOD2 mutation increases IgA transport, potentially by altering gut microfold cells from the gut, to impact gut inflammatio...
Kevadiya, Bhavesh D Machhi, Jatin Herskovitz, Jonathan Oleynikov, Maxim D Blomberg, Wilson R Bajwa, Neha Soni, Dhruvkumar Das, Srijanee Hasan, Mahmudul Patel, Milankumar
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Published in
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology
The COVID-19 pandemic has affected more than 38 million people world-wide by person to person transmission of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Therapeutic and preventative strategies for SARS-CoV-2 remains a significant challenge. Within the past several months, effective treatment options have emerged and now inclu...
Bachmann, Martin F. Mohsen, Mona O. Zha, Lisha Vogel, Monique Speiser, Daniel E.
Published in
npj Vaccines
Neutralizing antibody responses of SARS-CoV-2-infected patients may be low and of short duration. We propose here that coronaviruses employ a structural strategy to avoid strong and enduring antibody responses. Other viruses induce optimal and long-lived neutralizing antibody responses, thanks to 20 or more repetitive, rigid antigenic epitopes, spa...
Klumpp-Thomas, Carleen Kalish, Heather Drew, Matthew Hunsberger, Sally Snead, Kelly Fay, Michael P. Mehalko, Jennifer Shunmugavel, Anandakumar Wall, Vanessa Frank, Peter
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Published in
Nature Communications
Understanding the infection parameters and host responses against SARS-CoV-2 require data from large cohorts using standardized methods. Here, the authors optimize a serum ELISA protocol that has minimal cross-reactivity and flexible sample collection workflow in an attempt to standardize data generation and help inform on COVID-19 pandemic and imm...
Mao, Ying Qing Petritis, Brianne Huang, Ruo-Pan
Published in
Methods in molecular biology (Clifton, N.J.)
Sandwich-based antibody arrays enable the detection of multiple proteins simultaneously, thus offering a time- and cost-effective alternative to single-plex platforms. The protein of interest is "sandwiched" between an antibody that captures it to the array and a second antibody that is used for detection. Here we describe a 1-day procedure to proc...
Shah, Ashu Rauth, Sanchita Aithal, Abhijit Kaur, Sukhwinder Ganguly, Koelina Orzechowski, Catherine Varshney, Grish C Jain, Maneesh Batra, Surinder K
Published in
Theranostics
Over the past three decades, monoclonal antibodies (mAbs) have revolutionized the landscape of cancer therapy. Still, this benefit remains restricted to a small proportion of patients due to moderate response rates and resistance emergence. The field has started to embrace better mAb-based formats with advancements in molecular and protein engineer...
Rashed, Mohamed Z Kopechek, Jonathan A Priddy, Mariah C Hamorsky, Krystal T Palmer, Kenneth E Mittal, Nikhil Valdez, Joseph Flynn, Joseph Williams, Stuart J
Published in
Biosensors & bioelectronics
Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was classified as a pandemic by the World Health Organization and has caused over 550,000 deaths worldwide as of July 2020. Accurate and scalable point-of-care devices would increase screening, diagnosis, and monitoring of COVID-19 patients. Here, ...
Halushkina, Anastasiia Buchmann, William Jarroux, Nathalie Daniel, Régis
Published in
Methods in molecular biology (Clifton, N.J.)
The coupling of surface plasmon resonance imaging (SPRi) with mass spectrometry (MS) offers a very promising multidimensional analysis. This system takes advantage of the two well-established techniques: SPR, which allows for the analysis of biomolecular interactions through the determination of kinetic and thermodynamic constants, and MS, which ca...
