PCR enables sensitive and specific detection of infectious disease agents, but application in point-of-care diagnostic testing remains scarce. A compact tool that runs PCR assays in less than a few minutes and that relies on mass-producible, disposable reactors could revolutionize while-you-wait molecular testing. We here exploit well-established s...
PCR enables sensitive and specific detection of infectious disease agents, but application in point-of-care diagnostic testing remains scarce. A compact tool that runs PCR assays in less than a few minutes and that relies on mass-producible, disposable reactors could revolutionize while-you-wait molecular testing. We here exploit well-established s...
Simulated static in vitro digestion of tef injeras, sampled after different fermentation times, was performed to measure the dialyzable (D) and soluble nondialyzable total phenolic (TPC), total flavonoid contents (TFC), and their total antioxidants using ABTS, DPPH and FRAP assays. The %TPC and %TFC of D and SND fractions of the in vitro digested i...
Background: Ov16 serology is considered a reference method for Onchocerca volvulus epidemiological mapping. Given the suboptimal sensitivity of this test and the fact that seroconversion takes more than a year after infection, additional serological tests might be needed to guide onchocerciasis elimination programmes. Recently, two linear epitopes ...
The potential and current state-of-the-art of forensic SNP genotyping using nanopore sequencing was investigated with a panel of 16 tri-allelic single nucleotide polymorphisms (SNPs), multiplexing five samples per sequencing run. The sample set consisted of three single-source human genomic reference control DNA samples and two GEDNAP samples, simu...
Objectives: The antibiotic teicoplanin, used for the treatment of infections caused by Gram-positive bacteria, is highly bound to plasma proteins (percentage protein binding, %PB, around 90%) and therapeutic plasma levels of total teicoplanin are 10-100 mg/L. Because of the low free concentrations (i.e.
Simulated static in vitro digestion of tef injeras, sampled after different fermentation times, was performed to measure the dialyzable (D) and soluble nondialyzable total phenolic (TPC), total flavonoid contents (TFC), and their total antioxidants using ABTS, DPPH and FRAP assays. The %TPC and %TFC of D and SND fractions of the in vitro digested i...
Objectives: The antibiotic teicoplanin, used for the treatment of infections caused by Gram-positive bacteria, is highly bound to plasma proteins (percentage protein binding, %PB, around 90%) and therapeutic plasma levels of total teicoplanin are 10-100 mg/L. Because of the low free concentrations (i.e.
Objectives: The antibiotic teicoplanin, used for the treatment of infections caused by Gram-positive bacteria, is highly bound to plasma proteins (percentage protein binding, %PB, around 90%) and therapeutic plasma levels of total teicoplanin are 10-100 mg/L. Because of the low free concentrations (i.e.
