Anderson, Wesley A. Bosak, Alexander Hogberg, Helena T. Hartung, Thomas Moore, Michael J.
Published in
In Vitro Cellular & Developmental Biology. Animal
Microphysiological systems (MPS) designed to study the complexities of the peripheral and central nervous systems have made marked improvements over the years and have allowed researchers to assess in two and three dimensions the functional interconnectivity of neuronal tissues. The recent generation of brain organoids has further propelled the fie...
Gao, Wenling Liang, Tangzhao He, Ronghang Ren, Jianhua Yao, Hui Wang, Kun Zhu, Lei Xu, Yue
Published in
Stem cell research
Angiogenesis is an essential step in tissue engineering. MSC exosomes play an important role in angiogenesis. Functional biomolecules in exosomes vested by the culture microenvironment can be transferred to recipient cells and affects their effect. 3D culture can improve the proliferation and activity of MSCs. However, whether exosomes derived from...
Boucherit, Nicolas Gorvel, Laurent Olive, Daniel
Published in
Frontiers in Immunology
Over the past decade, immunotherapy has become a powerful and evident tool in the fight against cancers. Notably, the rise of checkpoint blockade using monoclonal antibodies (anti-CTLA4, anti-PD1) to avoid interaction between inhibitory molecules allowed the betterment of patient care. Indeed, immunotherapies led to increased overall survival in fo...
Muguruma, Masako Teraoka, Saeko Miyahara, Kana Ueda, Ai Asaoka, Mariko Okazaki, Miki Kawate, Takahiko Kuroda, Masahiko Miyagi, Yohei Ishikawa, Takashi
...
Published in
Biochemical and biophysical research communications
Three-dimensional (3D) culture reflects tumor biology complexities compared with two-dimensional (2D) culture. Thus, 3D culture has attracted attention in cell biology studies including drug sensitivity tests. Herein, we investigated differences in anticancer drug sensitivities between 2D and 3D culture systems in triple-negative breast cancer (TNB...
Suelzu, Caterina M. Conti, Virna Khalidy, Youssef Montagna, Sara Strusi, Gabriele Di Lecce, Rosanna Berni, Priscilla Basini, Giuseppina Ramoni, Roberto Grolli, Stefano
...
Published in
Cells
Mesenchymal stem cells (MSCs) have been recently introduced in veterinary medicine as a potential therapeutic tool for several pathologies. The large-scale in vitro expansion needed to ensure the preparation of a suitable number of MSCs for clinical application usually requires the use of xenogeneic supplements like the fetal bovine serum (FBS). Th...
Consales, Claudia Butera, Alessio Merla, Caterina Pasquali, Emanuela Lopresto, Vanni Pinto, Rosanna Pierdomenico, Maria Mancuso, Mariateresa Marino, Carmela Benassi, Barbara
...
Published in
Molecular neurobiology
We here characterize the response to the extremely low-frequency (ELF) magnetic field (MF, 50 Hz, 1 mT) of SH-SY5Y human neuroblastoma cells, cultured in a three-dimensional (3D) Alvetex® scaffold compared to conventional two-dimensional (2D) monolayers. We proved that the growing phenotype of proliferating SH-SY5Y cells is not affected by the cult...
Zhu, Huimin Qiao, Xin Liu, Wei Wang, Changyong Zhao, Yuwei
Published in
Frontiers in Neuroscience
In the process of constructing engineered neural tissues, we often use mixed primary neural cells, which contain microglia in the cell culture. However, the role that microglia play in the construction of engineered neural tissue has not been well studied. Here, we generated three-dimensional (3D) engineered neural tissues by silk fibroin/collagen ...
Venkataraman, Lalitha Fair, Summer R. McElroy, Craig A. Hester, Mark E. Fu, Hongjun
Published in
Stem Cell Reviews and Reports
Many neurodegenerative diseases (NDs) such as Alzheimer’s disease, Parkinson’s disease, frontotemporal dementia, amyotrophic lateral sclerosis and Huntington’s disease, are characterized by the progressive accumulation of abnormal proteinaceous assemblies in specific cell types and regions of the brain, leading to cellular dysfunction and brain dam...
Parodi, Valentina Jacchetti, Emanuela Bresci, Arianna Talone, Benedetta Valensise, Carlo M. Osellame, Roberto Cerullo, Giulio Polli, Dario Raimondi, Manuela T.
Published in
International Journal of Molecular Sciences
Three-dimensional culture systems and suitable substrates topographies demonstrated to drive stem cell fate in vitro by mechanical conditioning. For example, the Nichoid 3D scaffold remodels stem cells and shapes nuclei, thus promoting stem cell expansion and stemness maintenance. However, the mechanisms involved in force transmission and in bioche...
Abecasis, Bernardo Canhão, Pedro G.M. Almeida, Henrique V. Calmeiro, Tomás Fortunato, Elvira Gomes-Alves, Patrícia Serra, Margarida Alves, Paula M.
Published in
Frontiers in Bioengineering and Biotechnology
The combination of cardiomyocytes (CM) and non-myocyte cardiac populations, such as endothelial cells (EC), and mesenchymal cells (MC), has been shown to be critical for recapitulation of the human heart tissue for in vitro cell-based modeling. However, most of the current engineered cardiac microtissues still rely on either (i) murine/human limite...
