Laroche, Adrien Orsini Delgado, Maria Lucia Chalopin, Benjamin Cuniasse, Philippe Dubois, Steven Sierocki, Raphaël Gallais, Fabrice Debroas, Stéphanie Bellanger, Laurent Simon, Stéphanie
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Published in
mAbs
Here, we report the molecular engineering of nanobodies that bind with picomolar affinity to both SARS-CoV-1 and SARS-CoV-2 receptor-binding domains (RBD) and are highly neutralizing. We applied deep mutational engineering to VHH72, a nanobody initially specific for SARS-CoV-1 RBD with little cross-reactivity to SARS-CoV-2 antigen. We first identif...
Olga, Kolesnikova Amélie, Zachayus Simon, Pichard Judit, Osz Natacha, Rochel Paola, Rossolillo Isabelle, Kolb-Cheynel Nathalie, Troffer-Charlier Emmanuel, Compe Olivier, Bensaude
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Published in
Scientific Reports
The Baculovirus/insect cell expression system is a powerful technology for reconstitution of eukaryotic macromolecular assemblies. Most multigene expression platforms rely on Tn7-mediated transposition for transferring the expression cassette into the baculoviral genome. This allows a rigorous characterization of recombinant bacmids but involves mu...
Nicolas, Noémie de San Asokan, Aromal Rosa, Rafael Voisin, Sébastien Travers, Marie-Agnès Rocha, Gustavo Dantan, Luc Dorant, Yann Mitta, Guillaume Petton, Bruno
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Big defensins are two-domain antimicrobial peptides (AMPs) that have highly diversified in mollusks. Cg-BigDefs are expressed by immune cells in the oyster Crassostrea gigas, and their expression is dampened during the Pacific Oyster Mortality Syndrome (POMS), which evolves toward fatal bacteremia. We evaluated whether Cg-BigDefs contribute to the ...
Siddiqui, Bazla Rehman, Asim.ur. Haq, Ihsan-ul Al-Dossary, Amal A. Elaissari, Abdelhamid Ahmed, Naveed
Published in
International Journal of Pharmaceutics: X
• MSNs serve as an ideal candidate for gene/drug delivery with unique and excellent attributes. • MSNs surface can be functionalized using specific materials to impart unique structural features. • Functionalization of MSNs with stimuli-responsive molecules can act as gatekeepers by responding to the desired stimulus after uncapping. • These cappin...
Leducq, Valentin Jary, Aude Bridier-Nahmias, Antoine Daniel, Lena Zafilaza, Karen Damond, Florence Goldstein, Valérie Duval, Audrey Blanquart, François Calvez, Vincent
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Published in
Scientific Reports
France went through three deadly epidemic waves due to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causing major public health and socioeconomic issues. We proposed to study the course of the pandemic along 2020 from the outlook of two major Parisian hospitals earliest involved in the fight against COVID-19. Genome sequencing ...
Guiot, F. Praud, C. Quillard, S. Humbert, B. Ropers, M.-H. Paris, M. Terrisse, H.
This multi-scale and multi-technique work investigates the adsorption of phosphated species on the TiO2 anatase surface. Our original approach declines for the first time the progressive ethyl substitution of phosphates to identify the structure of complexes formed upon adsorption of these molecules on anatase in aqueous dispersions, under various ...
Nore, Alexandre Juarez-Martinez, Ariadna Clément, Julie Brun, Christine Diagouraga, Boubou Laroussi, Hamida Grey, Corinne Bourbon, Henri Marc Kadlec, Jan Robert, Thomas
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Abstract Meiosis requires the formation of programmed DNA double strand breaks (DSBs), essential for fertility and for generating genetic diversity. DSBs are induced by the catalytic activity of the TOPOVIL complex formed by SPO11 and TOPOVIBL. To ensure genomic integrity, DNA cleavage activity is tightly regulated, and several accessory factors (R...
Fiteni, Estelle Durand, Karine Gimenez, Sylvie Meagher Jr., Robert Legeai, Fabrice Kergoat, Gael Nègre, Nicolas dAlençon, Emmanuelle Nam, Ki Woong
BackgroundDivergent selection on host-plants is one of the main evolutionary forces driving ecological speciation in phytophagous insects. The ecological speciation might be challenging in the presence of gene flow and assortative mating because the direction of divergence is not necessarily the same between ecological selection (through host-plant...
Bernardi, Caroline Nogueira, Luísa Carle, Georges Alunni, Véronique Quatrehomme, Gérald
International audience
Kahilainen, Aapo Oostra, Vicencio Somervuo, Panu Minard, Guillaume Saastamoinen, Marjo
Published in
Molecular ecology
Predicting how climate change affects biotic interactions poses a challenge. Plant-insect herbivore interactions are particularly sensitive to climate change, as climate-induced changes in plant quality cascade into the performance of insect herbivores. Whereas the immediate survival of herbivore individuals depends on plastic responses to climate ...
