Wang, Yuan Wang, Sa Wu, Chunyan Chen, Xi Duan, Zhuhui Xu, Qian Jiang, Wen Xu, Lei Wang, Tingting Su, Lingkai
...
Published in
mSystems
Globally, dental caries is the most prevalent chronic oral disease and affects roughly half of all children. The aim of this report was to use metagenomic analyses to investigate the relationship between the oral microbiome and caries in preschool children. A total of 25 preschoolers, aged 3 to 5 years old with severe early childhood caries (ECC), ...
Hamilton, Trinity L Bennett, Annastacia C Murugapiran, Senthil K Havig, Jeff R
Published in
mSystems
Extant anoxygenic phototrophs are taxonomically, physiologically, and metabolically diverse and include examples from all seven bacterial phyla with characterized phototrophic members. pH, temperature, and sulfide are known to constrain phototrophs, but how these factors dictate the distribution and activity of specific taxa of anoxygenic phototrop...
Sharma, Kiran Bala Sharma, Manish Aggarwal, Suruchi Yadav, Amit Kumar Bhatnagar, Shinjini Vrati, Sudhanshu Kalia, Manjula
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mSystems
Basal autophagy is crucial for maintenance of cellular homeostasis. ATG5 is an essential protein for autophagosome formation, and its depletion has been extensively used as a tool to disrupt autophagy. Here, we characterize the impact of Atg5 deficiency on the cellular proteome of mouse embryonic fibroblasts (MEFs). Using a tandem mass tagging (TMT...
Niu, Chunyu Cheng, Chao Liu, Yanmin Huang, Shaolei Fu, Yanru Li, Peifeng
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mSystems
Lactobacillus strain SQ0048 isolated from bovine vagina has been shown to exhibit specific adherence to the epithelium and to produce inhibitory substances; however, the underlying mechanisms remain unclear. We cultured and identified primary bovine vaginal epithelial cells treated with SQ0048 to investigate the pathways involved in host cell respo...
Dumolin, Charles Aerts, Maarten Verheyde, Bart Schellaert, Simon Vandamme, Tim Van der Jeugt, Felix De Canck, Evelien Cnockaert, Margo Wieme, Anneleen D Cleenwerck, Ilse
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Published in
mSystems
The isolation of microorganisms from microbial community samples often yields a large number of conspecific isolates. Increasing the diversity covered by an isolate collection entails the implementation of methods and protocols to minimize the number of redundant isolates. Matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass ...
Zhang, Cui-Jing Pan, Jie Duan, Chang-Hai Wang, Yong-Ming Liu, Yang Sun, Jian Zhou, Hai-Chao Song, Xin Li, Meng
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mSystems
Mangroves, as a blue carbon reservoir, provide an environment for a variety of microorganisms. Mangroves lie in special locations connecting coastal and estuarine areas and experience fluctuating conditions, which are expected to intensify with climate change, creating a need to better understand the relative roles of stochastic and deterministic p...
Rubbens, Peter Schmidt, Marian L Props, Ruben Biddanda, Bopaiah A Boon, Nico Waegeman, Willem Denef, Vincent J
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mSystems
High-nucleic-acid (HNA) and low-nucleic-acid (LNA) bacteria are two operational groups identified by flow cytometry (FCM) in aquatic systems. A number of reports have shown that HNA cell density correlates strongly with heterotrophic production, while LNA cell density does not. However, which taxa are specifically associated with these groups, and ...
Small, Clayton M Currey, Mark Beck, Emily A Bassham, Susan Cresko, William A
Published in
mSystems
Multicellular organisms interact with resident microbes in important ways, and a better understanding of host-microbe interactions is aided by tools such as high-throughput 16S sequencing. However, rigorous evaluation of the veracity of these tools in a different context from which they were developed has often lagged behind. Our goal was to perfor...
McDonald, Daniel Kaehler, Benjamin Gonzalez, Antonio DeReus, Jeff Ackermann, Gail Marotz, Clarisse Huttley, Gavin Knight, Rob
Published in
mSystems
Meta-analyses at the whole-community level have been important in microbiome studies, revealing profound features that structure Earth's microbial communities, such as the unique differentiation of microbes from the mammalian gut relative to free-living microbial communities, the separation of microbiomes in saline and nonsaline environments, and t...
Walker, Alan W
Published in
mSystems
DNA sequence-based microbiome studies can be impacted by a range of different methodological artefacts. Contamination originating from laboratory kits and reagents can lead to erroneous results, particularly in samples containing a low microbial biomass. Minich and colleagues (mSystems 4:e00186-19, 2019, https://doi.org/10.1128/mSystems.00186-19) r...
