Laroche, Adrien Orsini Delgado, Maria Lucia Chalopin, Benjamin Cuniasse, Philippe Dubois, Steven Sierocki, Raphaël Gallais, Fabrice Debroas, Stéphanie Bellanger, Laurent Simon, Stéphanie
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Published in
mAbs
Here, we report the molecular engineering of nanobodies that bind with picomolar affinity to both SARS-CoV-1 and SARS-CoV-2 receptor-binding domains (RBD) and are highly neutralizing. We applied deep mutational engineering to VHH72, a nanobody initially specific for SARS-CoV-1 RBD with little cross-reactivity to SARS-CoV-2 antigen. We first identif...
Kailasan, Shweta Kant, Ravi Noonan-Shueh, Madeleine Kanipakala, Tulasikumari Liao, Grant Shulenin, Sergey Leung, Daisy W. Alm, Richard A. Adhikari, Rajan P. Amarasinghe, Gaya K.
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Published in
mAbs
Staphylococcus aureus carries an exceptional repertoire of virulence factors that aid in immune evasion. Previous single-target approaches for S. aureus -specific vaccines and monoclonal antibodies (mAbs) have failed in clinical trials due to the multitude of virulence factors released during infection. Emergence of antibiotic-resistant strains dem...
Geddie, Melissa L Kirpotin, Dmitri B Kohli, Neeraj Kornaga, Tad Boll, Bjoern Razlog, Maja Drummond, Daryl C Lugovskoy, Alexey A
Published in
mAbs
Antibody-directed nanotherapeutics (ADNs) represent a promising delivery platform for selective delivery of an encapsulated drug payload to the site of disease that improves the therapeutic index. Although both single-chain Fv (scFv) and Fab antibody fragments have been used for targeting, no platform approach applicable to any target has emerged. ...
de Graaf, Sebastiaan C. Hoek, Max Tamara, Sem Heck, Albert J. R.
Published in
mAbs
A key step in therapeutic and endogenous humoral antibody characterization is identifying the amino acid sequence. So far, this task has been mainly tackled through sequencing of B-cell receptor (BCR) repertoires at the nucleotide level. Mass spectrometry (MS) has emerged as an alternative tool for obtaining sequence information directly at the – m...
Ledsgaard, Line Laustsen, Andreas H. Pus, Urska Wade, Jack Villar, Pedro Boddum, Kim Slavny, Peter Masters, Edward W. Arias, Ana S. Oscoz, Saioa
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Published in
mAbs
The monocled cobra ( Naja kaouthia ) is among the most feared snakes in Southeast Asia due to its toxicity, which is predominantly derived from long-chain α-neurotoxins. The only specific treatment for snakebite envenoming is antivenom based on animal-derived polyclonal antibodies. Despite the lifesaving importance of these medicines, major limitat...
Hawkins, Alice Joyce, Callum Brady, Kevin Hold, Adam Smith, Alan Knight, Michael Howard, Conor van den Elsen, Jean Lawson, Alastair D.G. Macpherson, Alex
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mAbs
Cysteine-rich knob domains can be isolated from the ultralong heavy-chain complementarity-determining region (CDR) 3, which are unique to a subset of bovine antibodies, to create antibody fragments of ~4 kDa. Advantageously, the N- and C- termini of these small binding domains are in close proximity, and we propose that this may offer a practical r...
Wijdeven, Marloes A. van Geel, Remon Hoogenboom, Jorin H. Verkade, Jorge M. M. Janssen, Brian M. G. Hurkmans, Inge de Bever, Laureen van Berkel, Sander S. van Delft, Floris L.
Published in
mAbs
Liu, Yanyun Tsang, Kenny Mays, Michelle Hansen, Gale Chiecko, Jeffrey Crames, Maureen Wei, Yangjie Zhou, Weijie Fredrick, Chase Hu, James
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Published in
mAbs
Biotherapeutic optimization, whether to improve general properties or to engineer specific attributes, is a time-consuming process with uncertain outcomes. Conversely, Consensus Protein Design has been shown to be a viable approach to enhance protein stability while retaining function. In adapting this method for a more limited number of protein se...
Guimaraes Koch, Sofia S. Thorpe, Robin Kawasaki, Nana Lefranc, Marie-Paule Malan, Sarel Martin, Andrew C.R. Mignot, Gilles Plückthun, Andreas Rizzi, Menico Shubat, Stephanie
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Published in
mAbs
Appropriate nomenclature for all pharmaceutical substances is important for clinical development, licensing, prescribing, pharmacovigilance, and identification of counterfeits. Nonproprietary names that are unique and globally recognized for all pharmaceutical substances are assigned by the International Nonproprietary Names (INN) Programme of the ...
Schmitz, Samuel Schmitz, Emily A. Crowe, James E. Jr Meiler, Jens
Published in
mAbs
The human adaptive immune response enables the targeting of epitopes on pathogens with high specificity. Infection with a pathogen induces somatic hyper-mutation and B-cell selection processes that govern the shape and diversity of the antibody sequence landscape. To date, even the largest immunome repertoires of adaptive immune receptors acquired ...
