Carossino, Mariano Montanaro, Paige O'Connell, Aoife Kenney, Devin Gertje, Hans Grosz, Kyle A Kurnick, Susanna A Bosmann, Markus Saeed, Mohsan Balasuriya, Udeni B R
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Published in
bioRxiv : the preprint server for biology
Animal models recapitulating the distinctive features of severe COVID-19 are critical to enhance our understanding of SARS-CoV-2 pathogenesis. Transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) under the cytokeratin 18 promoter (K18-hACE2) represent a lethal model of SARS-CoV-2 infection. However, the cause(s) and mechanisms o...
Tian, Ruilin Samelson, Avi J Rezelj, Veronica V Chen, Merissa Ramadoss, Gokul N Guo, Xiaoyan Kain, Alice Mac Tran, Quang Dinh Lim, Shion A Lui, Irene
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bioRxiv : the preprint server for biology
SARS-CoV-2 infection of human cells is initiated by the binding of the viral Spike protein to its cell-surface receptor ACE2. We conducted an unbiased CRISPRi screen to uncover druggable pathways controlling Spike protein binding to human cells. We found that the protein BRD2 is an essential node in the cellular response to SARS-CoV-2 infection. BR...
Jaworski, Elizabeth Langsjoen, Rose M Judy, Barbara Newman, Patrick Plante, Jessica A Plante, Kenneth S Miller, Aaron L Zhou, Yiyang Swetnam, Daniele Dong, Jianli
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bioRxiv : the preprint server for biology
High-throughput genomics of SARS-CoV-2 is essential to characterize virus evolution and to identify adaptations that affect pathogenicity or transmission. While single-nucleotide variations (SNVs) are commonly considered as driving virus adaption, RNA recombination events that delete or insert nucleic acid sequences are also critical. Whole genome ...
Cheng, Kuoyuan Riva, Laura Sinha, Sanju Pal, Lipika Ray Nair, Nishanth Ulhas Martin-Sancho, Laura Chanda, Sumit K Ruppin, Eytan
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bioRxiv : the preprint server for biology
Tremendous progress has been made to control the COVID-19 pandemic, including the development and approval of vaccines as well as the drug remdesivir, which inhibits the SARS-CoV-2 virus that causes COVID-19. However, remdesivir confers only mild benefits to a subset of patients, and additional effective therapeutic options are needed. Drug repurpo...
Garushyants, Sofya K Rogozin, Igor B Koonin, Eugene V
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bioRxiv : the preprint server for biology
The appearance of multiple new SARS-CoV-2 variants during the winter of 2020-2021 is a matter of grave concern. Some of these new variants, such as B.1.351 and B.1.1.17, manifest higher infectivity and virulence than the earlier SARS-CoV-2 variants, with potential dramatic effects on the course of the COVID-19 pandemic. So far, analysis of new SARS...
McDonald, J Tyson Enguita, Francisco Javier Taylor, Deanne Griffin, Robert J Priebe, Waldemar Emmett, Mark R McGrath, Marisa Sajadi, Mohammad M Harris, Anthony D Clement, Jean
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bioRxiv : the preprint server for biology
MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional gene regulation that have a major impact on many diseases and provides an exciting avenue towards antiviral therapeutics. From patient transcriptomic data, we have discovered a circulating miRNA, miR-2392, that is directly involved with SARS-CoV-2 machinery during host in...
Sahoo, Debashis Katkar, Gajanan D Shimizu, Chisato Kim, Jihoon Khandelwal, Soni Tremoulet, Adriana H Kanegaye, John Bocchini, Joseph Das, Soumita Burns, Jane C
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bioRxiv : the preprint server for biology
A significant surge in cases of multisystem inflammatory syndrome in children (MIS-C, also called Pediatric Inflammatory Multisystem Syndrome - PIMS) has been observed amidst the COVID-19 pandemic. MIS-C shares many clinical features with Kawasaki disease (KD), although clinical course and outcomes are divergent. We analyzed whole blood RNA sequenc...
Leary, Shay Gaudieri, Silvana Parker, Matthew D Chopra, Abha James, Ian Pakala, Suman Alves, Eric John, Mina Lindsey, Benjamin B Keeley, Alexander J
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bioRxiv : the preprint server for biology
Genetic variations across the SARS-CoV-2 genome may influence transmissibility of the virus and the host’s anti-viral immune response, in turn affecting the frequency of variants over-time. In this study, we examined the adjacent amino acid polymorphisms in the nucleocapsid (R203K/G204R) of SARS-CoV-2 that arose on the background of the spike D61...
Gamble, Amandine Fischer, Robert J Morris, Dylan H Yinda, Kwe Claude Munster, Vincent J Lloyd-Smith, James O
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bioRxiv : the preprint server for biology
Decontamination helps limit environmental transmission of infectious agents. It is required for the safe re-use of contaminated medical, laboratory and personal protective equipment, and for the safe handling of biological samples. Heat treatment is a common decontamination method, notably used for viruses. We show that for liquid specimens (here, ...
Ullah, Irfan Prévost, Jérémie Ladinsky, Mark S Stone, Helen Lu, Maolin Anand, Sai Priya Beaudoin-Bussières, Guillaume Symmes, Kelly Benlarbi, Mehdi Ding, Shilei
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bioRxiv : the preprint server for biology
Neutralizing antibodies (NAbs) are effective in treating COVID-19 but the mechanism of immune protection is not fully understood. Here, we applied live bioluminescence imaging (BLI) to monitor the real-time effects of NAb treatment in prophylaxis and therapy of K18-hACE2 mice intranasally infected with SARS-CoV-2-nanoluciferase. We could visualize ...
