Djukic, Teodora Mladenovic, Maja Stanic-Vucinic, Dragana Radosavljevic, Jelena Smiljanic, Katarina Sabljic, Ljiljana Devic, Marija Cujic, Danica Vasovic, Tamara Simovic, Ana
...
Published in
Virology
Serological testing is important method for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production. SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58–419 aa) was ...
Kang, Xi Chen, Xi He, Ying Guo, Deyin Guo, Lin Zhong, Jin Shu, Hong-Bing
Published in
Virology
Mondal, Samiran Karmakar, Abhijit Mallick, Tamanna Begum, Naznin Ara
Published in
Virology
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Terry, James S. Anderson, Loran BR. Scherman, Michael S. McAlister, Carley E. Perera, Rushika Schountz, Tony Geiss, Brian J.
Published in
Virology
• Generation of novel monoclonal antibodies towards SARS-CoV-2 nucleocapsid protein. • Demonstration of utility in western blot, ELISA, and immunofluorescence assays. • Sequence analysis of antibody heavy and kappa chains. • Identification of a monoclonal antibody specific for SARS-CoV-2 nucleocapsid protein.
O'Brien, Amornrat Chen, Da-Yuan Hackbart, Matthew Close, Brianna J. O'Brien, Timothy E. Saeed, Mohsan Baker, Susan C.
Published in
Virology
• Coronavirus 3CLpro activity is efficiently measured using a luciferase-based biosensor. • Small molecule inhibitors of 3CLpro block activation of the biosensor. • Polyclonal antiserum raised against SARS-CoV 3CLpro recognizes SARS-CoV-2 3CLpro in WB and IF assays.
Stauft, Charles B. Lien, Christopher Z. Selvaraj, Prabhuanand Liu, Shufeng Wang, Tony T.
Published in
Virology
Dynamic tracking of variant frequencies among viruses circulating in the global pandemic has revealed the emergence and dominance of a D614G mutation in the SARS-CoV-2 spike protein. To address whether pandemic SARS-CoV-2 G614 variant has evolved to become more pathogenic, we infected adult hamsters (>10 months old) with two natural SARS-CoV-2 vari...
Qin, Pan Luo, Wen-Ting Su, Quan Zhao, Pengwei Zhang, Yuqi Wang, Bin Yang, Yong-Le Huang, Yao-Wei
Published in
Virology
• PDCoV accessory protein NS6 is expressed during infection in vivo. • A 13-KDa NS6 protein is detected in purified PDCoV virions. • NS6 is incorporated into PDCoV particles by immunogold labeling with anti-NS6. • Anti-NS6 does not exhibit neutralizing activity against PDCoV.
Detsika, Maria G. Ampelakiotou, Kleio Grigoriou, Eirini Psarra, Katherina Jahaj, Edison Roussos, Charis Dimopoulou, Ioanna Orfanos, Stylianos E. Tsirogianni, Alexandra Kotanidou, Anastasia
...
Published in
Virology
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Ratcliff, Jeremy Simmonds, Peter
Published in
Virology
- SARS-CoV-2 sequence variants contain an overabundance of C- > U transitions - C- > U transitions are the hallmark of the activity of APOBEC cytosine deaminases - Further work is needed to determine APOBEC's role in coronavirus evolution
Takeshita, Masaru Nishina, Naoshi Moriyama, Saya Takahashi, Yoshimasa Uwamino, Yoshifumi Nagata, Mika Aoki, Wataru Masaki, Katsunori Ishii, Makoto Saya, Hideyuki
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Published in
Virology
The pandemic of COVID-19 is still ongoing, and many studies on serum antibodies have been reported, however, there are few studies about asymptomatic and mild patients. In this study, we enrolled 44 COVID-19 patients with relatively mild disease and 48 pre-pandemic controls. We measured serum antibodies against extracellular domain, S1 domain, and ...
