Kruse, Martin Kohout, Susy C Hille, Bertil
Published in
The Journal of general physiology
Voltage-sensing phosphatases (VSPs) cleave both 3- and 5-phosphates from inositol phospholipids in response to membrane depolarization. When low concentrations of Ciona intestinalis VSP are expressed in Xenopus laevis oocytes, the 5-phosphatase reaction can be observed during large membrane depolarizations. When higher concentrations are expressed,...
Shi, Edward Y Yuan, Christine L Sipple, Matthew T Srinivasan, Jayasri Ptak, Christopher P Oswald, Robert E Nowak, Linda M
Published in
The Journal of general physiology
Glutamate is released from presynaptic nerve terminals in the central nervous system (CNS) and spreads excitation by binding to and activating postsynaptic iGluRs. Of the potential glutamate targets, tetrameric AMPA receptors mediate fast, transient CNS signaling. Each of the four AMPA subunits in the receptor channel complex is capable of binding ...
Moss, Richard L de Tombe, Pieter P Solaro, R John
Published in
The Journal of general physiology
Sobolevsky, Alexander I
Published in
The Journal of general physiology
Tilley, Drew C Angueyra, Juan M Eum, Kenneth S Kim, Heesoo Chao, Luke H Peng, Anthony W Sack, Jon T
Published in
The Journal of general physiology
Allosteric ligands modulate protein activity by altering the energy landscape of conformational space in ligand-protein complexes. Here we investigate how ligand binding to a K+ channel's voltage sensor allosterically modulates opening of its K+-conductive pore. The tarantula venom peptide guangxitoxin-1E (GxTx) binds to the voltage sensors of the ...
Jennings, Michael L
Published in
The Journal of general physiology
Transporters, pumps, and channels are proteins that catalyze the movement of solutes across membranes. The single-solute carriers, coupled exchangers, and coupled cotransporters that are collectively known as transporters are distinct from conductive ion channels, water channels, and ATP-hydrolyzing pumps. The main conceptual framework for studying...
Touska, Filip Turnquist, Brian Vlachova, Viktorie Reeh, Peter W Leffler, Andreas Zimmermann, Katharina
Published in
The Journal of general physiology
Damage-sensing nociceptors in the skin provide an indispensable protective function thanks to their specialized ability to detect and transmit hot temperatures that would block or inflict irreversible damage in other mammalian neurons. Here we show that the exceptional capacity of skin C-fiber nociceptors to encode noxiously hot temperatures depend...
Pierce, Jonathan T
Published in
The Journal of general physiology
The role of ion channels in cell excitability was first revealed in a series of voltage clamp experiments by Hodgkin and Huxley in the 1950s. However, it was not until the 1970s that patch-clamp recording ushered in a revolution that allowed physiologists to witness how ion channels flicker open and closed at angstrom scale and with microsecond res...
Liin, Sara I Lund, Per-Eric Larsson, Johan E Brask, Johan Wallner, Björn Elinder, Fredrik
Published in
The Journal of general physiology
Voltage-gated ion channels are key molecules for the generation of cellular electrical excitability. Many pharmaceutical drugs target these channels by blocking their ion-conducting pore, but in many cases, channel-opening compounds would be more beneficial. Here, to search for new channel-opening compounds, we screen 18,000 compounds with high-thr...
Melzer, Werner
Published in
The Journal of general physiology
Calcium ions control multiple physiological functions by binding to extracellular and intracellular targets. One of the best-studied Ca2+-dependent functions is contraction of smooth and striated muscle tissue, which results from Ca2+ ligation to calmodulin and troponin C, respectively. Ca2+ signaling typically involves flux of the ion across membr...
