Correction: Role of the early secretory pathway in SARS-CoV-2 infection.
Published in The Journal of cell biology
ULK complex organization in autophagy by a C-shaped FIP200 N-terminal domain dimer.
Published in The Journal of cell biology
The autophagy-initiating human ULK complex consists of the kinase ULK1/2, FIP200, ATG13, and ATG101. Hydrogen-deuterium exchange mass spectrometry was used to map their mutual interactions. The N-terminal 640 residues (NTD) of FIP200 interact with the C-terminal IDR of ATG13. Mutations in these regions abolish their interaction. Negative stain EM a...
TORC1 inactivation stimulates autophagy of nucleoporin and nuclear pore complexes.
Published in The Journal of cell biology
The mechanisms underlying turnover of the nuclear pore complex (NPC) and the component nucleoporins (Nups) are still poorly understood. In this study, we found that the budding yeast Saccharomyces cerevisiae triggers NPC degradation by autophagy upon the inactivation of Tor kinase complex 1. This degradation largely depends on the selective autopha...
Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.
Published in The Journal of cell biology
In the absence of Hedgehog ligand, patched-1 (Ptch1) localizes to cilia and prevents ciliary accumulation and activation of smoothened (Smo). Upon ligand binding, Ptch1 is removed from cilia, and Smo is derepressed and accumulates in cilia where it activates signaling. The mechanisms regulating these dynamic movements are not well understood, but d...
A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy.
Published in The Journal of cell biology
Autophagy degrades cytoplasmic cargo by its delivery to lysosomes within double membrane autophagosomes. Synthesis of the phosphoinositide PI(3)P by the autophagic class III phosphatidylinositol-3 kinase complex I (PI3KC3-C1) and conjugation of ATG8/LC3 proteins to phagophore membranes by the ATG12-ATG5-ATG16L1 (E3) complex are two critical steps i...
Stress-induced phosphorylation of CLIP-170 by JNK promotes microtubule rescue.
Published in The Journal of cell biology
The stress-induced c-Jun N-terminal kinase (JNK) controls microtubule dynamics by enhancing both microtubule growth and rescues. Here, we show that upon cell stress, JNK directly phosphorylates the microtubule rescue factor CLIP-170 in its microtubule-binding domain to increase its rescue-promoting activity. Phosphomimetic versions of CLIP-170 enha...
LUZP1 and the tumor suppressor EPLIN modulate actin stability to restrict primary cilia formation.
Published in The Journal of cell biology
Cilia and flagella are microtubule-based cellular projections with important sensory and motility functions. Their absence or malfunction is associated with a growing number of human diseases collectively referred to as ciliopathies. However, the fundamental mechanisms underpinning cilia biogenesis and functions remain only partly understood. Here,...
Functional modulation of IFT kinesins extends the sensory repertoire of ciliated neurons in Caenorhabditis elegans.
Published in The Journal of cell biology
The diversity of sensory cilia on Caenorhabditis elegans neurons allows the animal to detect a variety of sensory stimuli. Sensory cilia are assembled by intraflagellar transport (IFT) kinesins, which transport ciliary precursors, bound to IFT particles, along the ciliary axoneme for incorporation into ciliary structures. Using fluorescence microsc...
Susana Godinho: Placing cell biology at the center of cancer research.
Published in The Journal of cell biology
Godinho investigates the role centrosomes play in cancer cell biology. © 2020 Rockefeller University Press.
The stress-responsive gene GDPGP1/mcp-1 regulates neuronal glycogen metabolism and survival.
...Published in The Journal of cell biology
Maladaptive responses to stress might play a role in the sensitivity of neurons to stress. To identify novel cellular responses to stress, we performed transcriptional analysis in acutely stressed mouse neurons, followed by functional characterization in Caenorhabditis elegans. In both contexts, we found that the gene GDPGP1/mcp-1 is down-regulated...
