Baker, Martin J Cooke, Mariana Kreider-Letterman, Gabriel Garcia-Mata, Rafael Janmey, Paul A Kazanietz, Marcelo G
Published in
The Journal of biological chemistry
A large number of aggressive cancer cell lines display elevated levels of activated Rac1, a small GTPase widely implicated in cytoskeleton reorganization, cell motility, and metastatic dissemination. A commonly accepted methodological approach for detecting Rac1 activation in cancer cells involves the use of a conformation-sensitive antibody that d...
Lee, Soon Goo Harline, Kate Abar, Orchid Akadri, Sakirat O Bastian, Alexander G Chen, Hui-Yuan S Duan, Michael Focht, Caroline M Groziak, Amanda R Kao, Jesse
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Published in
The Journal of biological chemistry
Aldehyde dehydrogenases are versatile enzymes that serve a range of biochemical functions. Although traditionally considered metabolic housekeeping enzymes because of their ability to detoxify reactive aldehydes, like those generated from lipid peroxidation damage, the contributions of these enzymes to other biological processes are widespread. For...
Nagareddy, Bhavika Khan, Arafat Kim, Hyungjin
Published in
The Journal of biological chemistry
Fanconi anemia (FA) is a chromosome instability syndrome of children caused by inherited mutations in one of FA genes, which together constitute a DNA interstrand cross-link (ICL) repair, or the FA pathway. Monoubiquitination of Fanconi anemia group D2 protein (FANCD2) by the multisubunit ubiquitin E3 ligase, the FA core complex, is an obligate ste...
Linden, Katrina J Callis, Judy
Published in
The Journal of biological chemistry
In a single vascular plant species, the ubiquitin system consists of thousands of different proteins involved in attaching ubiquitin to substrates, recognizing or processing ubiquitinated proteins, or constituting or regulating the 26S proteasome. The ubiquitin system affects plant health, reproduction, and responses to the environment, processes t...
Laverde, Eduardo E Lai, Yanhao Leng, Fenfei Balakrishnan, Lata Freudenreich, Catherine H Liu, Yuan
Published in
The Journal of biological chemistry
Trinucleotide repeat (TNR) expansion and deletion are responsible for over 40 neurodegenerative diseases and associated with cancer. TNRs can undergo somatic instability that is mediated by DNA damage and repair and gene transcription. Recent studies have pointed toward a role for R-loops in causing TNR expansion and deletion, and it has been shown...
Wedemeyer, Michael J Mahn, Sarah A Getschman, Anthony E Crawford, Kyler S Peterson, Francis C Marchese, Adriano McCorvy, John D Volkman, Brian F
Published in
The Journal of biological chemistry
The human chemokine family consists of 46 protein ligands that induce chemotactic cell migration by activating a family of 23 G protein-coupled receptors. The two major chemokine subfamilies, CC and CXC, bind distinct receptor subsets. A sequence motif defining these families, the X position in the CXC motif, is not predicted to make significant co...
Chen, Joseph C Luu, Amanda R Wise, Nathan Angelis, Rolando De Agrawal, Vishal Mangini, Linley Vincelette, Jon Handyside, Britta Sterling, Harry Lo, Melanie J
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Published in
The Journal of biological chemistry
Autosomal recessive mutations in the galactosidase β1 (GLB1) gene cause lysosomal β-gal deficiency, resulting in accumulation of galactose-containing substrates and onset of the progressive and fatal neurodegenerative lysosomal storage disease, GM1 gangliosidosis. Here, an enzyme replacement therapy (ERT) approach in fibroblasts from GM1 gangliosid...
Platsaki, Semeli Zhou, Xin Pinan-Lucarré, Bérangère Delauzun, Vincent Tu, Haijun Mansuelle, Pascal Fourquet, Patrick Bourne, Yves Bessereau, Jean-Louis Marchot, Pascale
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Published in
The Journal of biological chemistry
Punctin/MADD-4, a member of the ADAMTSL extracellular matrix protein family, was identified as an anterograde synaptic organizer in the nematode Caenorhabditis elegans. At GABAergic neuromuscular junctions, the short isoform MADD-4B binds the ectodomain of neuroligin NLG-1, itself a postsynaptic organizer of inhibitory synapses. To identify the mol...
Flowers, Sarah A Thomsson, Kristina A Ali, Liaqat Huang, Shan Mthembu, Yolanda Regmi, Suresh C Holgersson, Jan Schimdt, Tannin A Rolfson, Ola Bjorkman, Lena I
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Published in
The Journal of biological chemistry
The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development. Lubricin's ability to reduce friction is related to its glycosylation consisting of sialylated and unsialylated Tn-antigens, core-1 and core-2 structures. Th...
Söderholm, Annika Newton, Matilda S Patrick, Wayne M Selmer, Maria
Published in
The Journal of biological chemistry
In tryptophan biosynthesis, the reaction catalyzed by the enzyme indole-3-glycerol phosphate synthase (IGP synthase, IGPS) starts with a condensation step where the substrate's carboxylated phenyl group makes a nucleophilic attack to form the pyrrole ring of the indole, followed by a decarboxylation which restores the aromaticity of the phenyl. IGP...
