Kalous, Kelsey S Wynia-Smith, Sarah L Summers, Steven B Smith, Brian C
Published in
The Journal of biological chemistry
Sirtuins (e.g., human Sirt1-7) catalyze the removal of acyl groups from lysine residues in proteins in an NAD+-dependent manner, and loss of sirtuin deacylase activity correlates with the development of aging-related diseases. Although multiple reports suggest that sirtuin activity is regulated by oxidative posttranslational modifications of cystei...
Kapoor, Aastha Chen, Carolyn G Iozzo, Renato V
Published in
The Journal of biological chemistry
Endorepellin, the C-terminal fragment of the heparan sulfate proteoglycan perlecan, influences various signaling pathways in endothelial cells by binding to VEGFR2. In this study, we discovered that soluble endorepellin activates the canonical stress signaling pathway consisting of PERK, eIF2α, ATF4 and GADD45α. Specifically, endorepellin evoked tr...
Bradley, Michelle C Yang, Krista Fernández-Del-Río, Lucia Ngo, Jennifer Ayer, Anita Tsui, Hui S Novales, Noelle Alexa Stocker, Roland Shirihai, Orian S Barros, Mario H
...
Published in
The Journal of biological chemistry
Coenzyme Q (Qn) is a vital lipid component of the electron transport chain that functions in cellular energy metabolism and as a membrane antioxidant. In the yeast Saccharomyces cerevisiae, coq1-coq9 deletion mutants are respiratory incompetent, sensitive to lipid peroxidation stress, and unable to synthesize Q6 The yeast coq10 deletion mutant is a...
Rose, Jason J Bocian, Kaitlin A Xu, Qinzi Wang, Ling DeMartino, Anthony W Chen, Xiukai Corey, Catherine G Guimarães, Danielle A Azarov, Ivan Huang, Xueyin N
...
Published in
The Journal of biological chemistry
Carbon monoxide (CO) remains the most common cause of human poisoning. The consequences of CO poisoning include cardiac dysfunction, brain injury, and death. CO causes toxicity by binding to hemoglobin and by inhibiting mitochondrial cytochrome c oxidase (CcO), thereby decreasing oxygen delivery and inhibiting oxidative phosphorylation. We have rec...
Manska, Salomé Octaviano, Rionna Rossetto, Cyprian C
Published in
The Journal of biological chemistry
Nucleoside analogs are a valuable experimental tool. Incorporation of these molecules into newly synthesized DNA (i.e. pulse-labeling) is used to monitor cell proliferation or to isolate nascent DNA. Some of the most common nucleoside analogs used for pulse-labeling of DNA in cells are the deoxypyrimidine analogs 5-ethynyl-2'-deoxyuridine (EdU) and...
Kabra, Ashish Rumpa, Efsita Li, Ying
Published in
The Journal of biological chemistry
Deubiquitinases deconjugate ubiquitin modifications from target proteins and are involved in many cellular processes in eukaryotes. The functions of deubiquitinases are regulated by post-translational modifications, mainly phosphorylation and ubiquitination. Post-translational modifications can result in subtle changes in structural and dynamic pro...
Huang, Wei Ray, Paramita Ji, Wenbin Wang, Zhuwen Nancarrow, Derek Chen, Guoan Galban, Stefanie Lawrence, Theodore S Beer, David G Rehemtulla, Alnawaz
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Published in
The Journal of biological chemistry
We previously reported that overexpression of cytochrome P450 family 24 subfamily A member 1 (CYP24A1) increases lung cancer cell proliferation by activating RAS signaling and that CYP24A1 knockdown inhibits tumor growth. However, the mechanism of CYP24A1-mediated cancer cell proliferation remains unclear. Here, we conducted cell-synchronization an...
Abe, Yuichi Honsho, Masanori Kawaguchi, Ryoko Matsuzaki, Takashi Ichiki, Yayoi Fujitani, Masashi Fujiwara, Kazushirou Hirokane, Masaaki Oku, Masahide Sakai, Yasuyoshi
...
Published in
The Journal of biological chemistry
The peroxisome is a subcellular organelle that functions in essential metabolic pathways, including biosynthesis of plasmalogens, fatty acid β-oxidation of very-long-chain fatty acids, and degradation of hydrogen peroxide. Peroxisome biogenesis disorders (PBDs) manifest as severe dysfunction in multiple organs, including the central nervous system ...
Shimada, Atsuhiro Etoh, Yuki Kitoh-Fujisawa, Rika Sasaki, Ai Shinzawa-Itoh, Kyoko Hiromoto, Takeshi Yamashita, Eiki Muramoto, Kazumasa Tsukihara, Tomitake Yoshikawa, Shinya
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Published in
The Journal of biological chemistry
Cytochrome c oxidase (CcO) reduces O2 to water, coupled with a proton-pumping process. The structure of the O2-reduction site of CcO contains two reducing equivalents, Fe a3 2+ and CuB 1+, and suggests that a peroxide-bound state (Fe a3 3+-O--O--CuB 2+) rather than an O2-bound state (Fe a3 2+-O2) is the initial catalytic intermediate. Unexpectedly,...
Shen, Yaoyao Sun, Fan Zhang, Liu Cheng, Yijia Zhu, Hongrui Wang, Shu-Ping Jiao, Wei-Hua Leadlay, Peter F Zhou, Yongjun Lin, Hou-Wen
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Published in
The Journal of biological chemistry
Neoantimycins are anticancer compounds of 15-membered ring antimycin-type depsipeptides. They are biosynthesized by a hybrid multimodular protein complex of non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS), typically from the starting precursor 3-formamidosalicylate (3-FAS). Examining fermentation extracts of Streptomyces congl...
