WAKE, Yui KANEKO, Takehito
Published in
The Journal of Reproduction and Development
Genome editing technology contributes to the quick and highly efficient production of genetically engineered animals. These animals are helpful in clarifying the mechanism of human disease. Recently, a new electroporation technique (TAKE: Technique for animal knockout system by electroporation) was developed to produce genome-edited animals by intr...
TONAI, Shingo KAWABATA, Akane NAKANISHI, Tomoya LEE, Joo Yeon OKAMOTO, Asako SHIMADA, Masayuki YAMASHITA, Yasuhisa
Published in
The Journal of Reproduction and Development
Iron is important for many cellular functions, including ATP synthesis and cell proliferation. Insufficient of iron in the diet causes iron deficiency anemia (IDA), which often occurs in people living in the world. Since 50% of women with IDA show amenorrhea, the relationship of between iron deficiency and reproductive function was assessed using m...
SENOO, Manami HOZOJI, Hiroshi ISHIKAWA-YAMAUCHI, Yu TAKIJIRI, Takashi OHTA, Sho UKAI, Tomoyo KABATA, Mio YAMAMOTO, Takuya YAMADA, Yasuhiro IKAWA, Masahito
...
Published in
The Journal of Reproduction and Development
PTBP1, a well-conserved RNA-binding protein, regulates cellular development by tuning posttranscriptional mRNA modification such as alternative splicing (AS) or mRNA stabilization. We previously revealed that the loss of Ptbp1 in spermatogonia causes the dysregulation of spermatogenesis, but the molecular mechanisms by which PTBP1 regulates spermat...
NOGUCHI, Michiko SUZUKI, Takehito SATO, Reiichiro SASAKI, Yosuke KANEKO, Kazuyuki
Published in
The Journal of Reproduction and Development
This study aimed to determine if lactation can be induced by exogenous hormonal treatment in non-pregnant sows. In experiment 1, pseudopregnant animals were divided into four groups and given: 1) 5 mg of estradiol dipropionate (EDP) 5 days before (n = 4), 2) 5 mg of EDP 10 days before (n = 3), 3) 10 mg of EDP 5 days before (n = 3) or 4) 10 mg of ED...
KOYAMA, Keisuke TAKAHASHI, Takayoshi
Published in
The Journal of Reproduction and Development
Modern genetic improvement in dairy cattle is directed towards improvement of fertility; however, reproduction traits generally exhibit a genetic antagonism with milk yield. Herein, we aimed to clarify the effects of sire predicted transmitting ability (PTA) for daughter pregnancy rate (DPR) on the reproductive performance and milk yield of daughte...
ENDO, Natsumi RAHAYU, Larasati Puji YAMAMURA, Takashi TANAKA, Hitoshi TANAKA, Tomomi
Published in
The Journal of Reproduction and Development
The objective of the present study was to develop and evaluate a sustained release vaginal progesterone (P4) capsule containing a mixture of mucoadhesive polymer and silicone fluid. Goats were administered a gelatin capsule containing 0.4 g of P4 mixed in silicone fluid and either a hydroxypropylmethylcellulose (HM) or polyaclil starch (PA) base. T...
INOUE, Rei HARADA, Kana WAKAYAMA, Sayaka OOGA, Masatoshi WAKAYAMA, Teruhiko
Published in
The Journal of Reproduction and Development
Mouse oocytes are generally collected after euthanasia. However, if oocytes were collected without euthanasia, then mice could be used to collect oocytes again after recovery. This condition is especially useful for mice that are genotypically rare. In this study, we examined the reusability of mice after collecting oocytes via a surgical operation...
MAYAMA, Shota HAMAZAKI, Nobuhiko MARUYAMA, Yuki MATSUBARA, Shin KIMURA, Atsushi P.
Published in
The Journal of Reproduction and Development
Specific gene expression in granulosa cells is key for the function of ovary, but the molecular mechanism of transcriptional activation is not well studied. Here we investigated the regulatory mechanism of the mouse stearoyl-CoA desaturase 2 ( Scd2 ) gene encoding an enzyme for lipid metabolism. Northern blot and in situ hybridization indicated tha...
HIRADATE, Yuki HARA, Kenshiro TANEMURA, Kentaro
Published in
The Journal of Reproduction and Development
Previously, we revealed that neurotensin (NTS) derived from the oviduct and uterus can function during fertilization. However, little is known about NTS actions on the pre-implantation embryo after fertilization. Here, we found that pro- Nts mRNA is expressed in the oviduct and uterus during when preimplantation embryos develop and an increase in m...
GARCIA-ISPIERTO, Irina LÓPEZ-GATIUS, Fernando
Published in
The Journal of Reproduction and Development
We examined the effects of a single or 2.5-fold dose of dephereline [a gonadotropin-releasing hormone (GnRH) analogue] as well as the drainage of the smaller follicle at the time of insemination on ovulation in dairy cows with two follicles of pre-ovulatory size in the same ovary. The three study groups included 220 monovular cows (control), 110 no...
