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Domain structure of secretin PulD revealed by limited proteolysis and electron microscopy.

Nouwen, N Stahlberg, H Pugsley, A P Engel, A

Secretins, a superfamily of multimeric outer membrane proteins, mediate the transport of large macromolecules across the outer membrane of Gram-negative bacteria. Limited proteolysis of secretin PulD from the Klebsiella oxytoca pullulanase secretion pathway showed that it consists of an N-terminal domain and a protease-resistant C-terminal domain t...

High resolution AFM topographs of the Escherichia coli water channel aquaporin Z.

Scheuring, S Ringler, P Borgnia, M Stahlberg, H Müller, D J Agre, P Engel, A

Published in The EMBO journal

Aquaporins form a large family of membrane channels involved in osmoregulation. Electron crystallography has shown monomers to consist of six membrane spanning alpha-helices confirming sequence based predictions. Surface exposed loops are the least conserved regions, allowing differentiation of aquaporins. Atomic force microscopy was used to image ...

The Sir4 H‐ BRCT domain interacts with phospho‐proteins to sequester and repress yeast heterochromatin

Deshpande, Ishan Keusch, Jeremy J Challa, Kiran Iesmantavicius, Vytautas Gasser, Susan M Gut, Heinz

Published in The EMBO Journal

Loss of the maintenance methyltransferase, xD nmt1, induces apoptosis in Xenopus embryos

Stancheva, Irina Hensey, Carmel Meehan, Richard R

Published in The EMBO Journal

Retraction: Regulation of MBD1-mediated transcriptional repression by SUMO and PIAS proteins.

Lyst, Matthew J Nan, Xinsheng Stancheva, Irina

Published in The EMBO journal

Phosphatidylinositol 4,5‐bisphosphate controls Rab7 and PLEKHM 1 membrane cycling during autophagosome–lysosome fusion

Baba, Takashi Toth, Daniel J Sengupta, Nivedita Kim, Yeun Ju Balla, Tamas

Published in The EMBO Journal

Retraction: "CITRX thioredoxin interacts with the tomato Cf-9 resistance protein and negatively regulates defence".

Rivas, Susana Rougon-Cardoso, Alejandra Smoker, Matthew Schauser, Leif Yoshioka, Hirofumi Jones, Jonathan Dg

Published in The EMBO journal

Function and dysfunction of the PI system in membrane trafficking

Vicinanza, Mariella D'Angelo, Giovanni Di Campli, Antonella De Matteis, Maria Antonietta

Published in The EMBO Journal

The phosphoinositides (PIs) function as efficient and finely tuned switches that control the assembly–disassembly cycles of complex molecular machineries with key roles in membrane trafficking. This important role of the PIs is mainly due to their versatile nature, which is in turn determined by their fast metabolic interconversions. PIs can be tig...

Large conformational changes in MutS during DNA scanning, mismatch recognition and repair signaling

Qiu, Ruoyi DeRocco, Vanessa C Harris, Credle Sharma, Anushi Hingorani, Manju M Erie, Dorothy A Weninger, Keith R

Published in The EMBO Journal

STIM1 (c)AMPs up melanogenesis.

Soboloff, Jonathan Gligorijevic, Bojana Zaidi, M Raza

Published in The EMBO journal

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