Byrne, Dominic P Clarke, Christopher J Brownridge, Philip J Kalyuzhnyy, Anton Perkins, Simon Campbell, Amy Mason, David Jones, Andrew R Eyers, Patrick A Eyers, Claire E
...
Published in
The Biochemical journal
Polo-like kinase 4 (PLK4) is the master regulator of centriole duplication in metazoan organisms. Catalytic activity and protein turnover of PLK4 are tightly coupled in human cells, since changes in PLK4 concentration and catalysis have profound effects on centriole duplication and supernumerary centrosomes, which are associated with aneuploidy and...
Du, Min Wang, Guozheng Barsukov, Igor L Gross, Stephane R Smith, Richard Rudland, Philip S
Published in
The Biochemical journal
Overexpression of S100P promotes breast cancer metastasis in animals and elevated levels in primary breast cancers are associated with poor patient outcomes. S100P can differentially interact with nonmuscle myosin (NM) isoforms (IIA > IIC > IIB) leading to the redistribution of actomyosin filaments to enhance cell migration. Using COS-7 cells which...
Harrington, John M Chou, Hui-Ting Gutsmann, Thomas Gelhaus, Christoph Stahlberg, Henning Leippe, Matthias Armstrong, Peter B
Published in
The Biochemical journal
The pentraxins are a family of highly conserved plasma proteins of metazoans known to function in immune defence. The canonical members, C-reactive protein and serum amyloid P component, have been identified in arthropods and humans. Mammalian pentraxins are known to bind lipid bilayers, and a pentraxin representative from the American horseshoe cr...
Friedrich, Michael G. Wang, Zhen Schey, Kevin L. Truscott, Roger J. W.
Published in
The Biochemical journal
Long-lived proteins are present in numerous tissues within the human body. With age, they deteriorate, often leading to the formation of irreversible modifications such as peptide bond cleavage and covalent crosslinking. Currently understanding of the mechanism of formation of these crosslinks is limited. As part of an ongoing study, proteomics was...
Gamiz-Arco, Gloria Risso, Valeria A Candel, Adela M Inglés-Prieto, Alvaro Romero-Romero, Maria L Gaucher, Eric A Gavira, Jose A Ibarra-Molero, Beatriz Sanchez-Ruiz, Jose M
Published in
The Biochemical journal
Evolution involves not only adaptation, but also the degradation of superfluous features. Many examples of degradation at the morphological level are known (vestigial organs, for instance). However, the impact of degradation on molecular evolution has been rarely addressed. Thioredoxins serve as general oxidoreductases in all cells. Here, we report...
Davydova, Nadezhda Y Dangi, Bikash Maldonado, Marc A Vavilov, Nikita E Zgoda, Victor G Davydov, Dmitri R
Published in
The Biochemical journal
In this study, we investigate the ability of ethanol-inducible CYP2E1 to interact with other cytochrome P450 species and affect the metabolism of their substrates. As a model system, we used CYP2E1-enriched human liver microsomes (HLM) obtained by the incorporation of purified CYP2E1. Using a technique based on homo-FRET in oligomers of CYP2E1 labe...
Strack, Karen Lauri, Natalia Maté, Sabina María Saralegui, Andrés Muñoz-Garay, Carlos Schwarzbaum, Pablo J Herlax, Vanesa
Published in
The Biochemical journal
Alpha hemolysin (HlyA) is the major virulence factor of uropathogenic Escherichia coli (UPEC) strains. Once in circulation, a low concentration of the toxin induces an increase in intracellular calcium that activates calpains - which proteolyse cytoskeleton proteins - and also favours the exposure of phosphatidylserine (PS) in the outer leaflet of ...
Soubeyrand, Eric Kelly, Megan Keene, Shea A Bernert, Ann C Latimer, Scott Johnson, Timothy S Elowsky, Christian Colquhoun, Thomas A Block, Anna K Basset, Gilles J
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Published in
The Biochemical journal
Plants have evolved the ability to derive the benzenoid moiety of the respiratory cofactor and antioxidant, ubiquinone (coenzyme Q), either from the β-oxidative metabolism of p-coumarate or from the peroxidative cleavage of kaempferol. Here, isotopic feeding assays, gene co-expression analysis and reverse genetics identified Arabidopsis 4-COUMARATE...
Si, Meiru Chen, Can Wei, Zengfan Gong, Zhijin Li, GuiZhi Yao, Shumin
Published in
The Biochemical journal
MarR (multiple antibiotic resistance regulator) proteins are a family of transcriptional regulators that is prevalent in Corynebacterium glutamicum. Understanding the physiological and biochemical function of MarR homologs in C. glutamicum has focused on cysteine oxidation-based redox-sensing and substrate metabolism-involving regulators. In this s...
Uhrik, Lukas Wang, Lixiao Haronikova, Lucia Medina-Medina, Ixaura Rebolloso-Gomez, Yolanda Chen, Sa Vojtesek, Borivoj Fahraeus, Robin Hernychova, Lenka Olivares-Illana, Vanesa
...
Published in
The Biochemical journal
Allosteric changes imposed by post-translational modifications regulate and differentiate the functions of proteins with intrinsic disorder regions. HDM2 is a hub protein with a large interactome and with different cellular functions. It is best known for its regulation of the p53 tumour suppressor. Under normal cellular conditions, HDM2 ubiquitina...
