Du, Min Wang, Guozheng Barsukov, Igor L Gross, Stephane R Smith, Richard Rudland, Philip S
Published in
The Biochemical journal
Overexpression of S100P promotes breast cancer metastasis in animals and elevated levels in primary breast cancers are associated with poor patient outcomes. S100P can differentially interact with nonmuscle myosin (NM) isoforms (IIA > IIC > IIB) leading to the redistribution of actomyosin filaments to enhance cell migration. Using COS-7 cells which...
Gamiz-Arco, Gloria Risso, Valeria A Candel, Adela M Inglés-Prieto, Alvaro Romero-Romero, Maria L Gaucher, Eric A Gavira, Jose A Ibarra-Molero, Beatriz Sanchez-Ruiz, Jose M
Published in
The Biochemical journal
Evolution involves not only adaptation, but also the degradation of superfluous features. Many examples of degradation at the morphological level are known (vestigial organs, for instance). However, the impact of degradation on molecular evolution has been rarely addressed. Thioredoxins serve as general oxidoreductases in all cells. Here, we report...
Davydova, Nadezhda Y Dangi, Bikash Maldonado, Marc A Vavilov, Nikita E Zgoda, Victor G Davydov, Dmitri R
Published in
The Biochemical journal
In this study, we investigate the ability of ethanol-inducible CYP2E1 to interact with other cytochrome P450 species and affect the metabolism of their substrates. As a model system, we used CYP2E1-enriched human liver microsomes (HLM) obtained by the incorporation of purified CYP2E1. Using a technique based on homo-FRET in oligomers of CYP2E1 labe...
Strack, Karen Lauri, Natalia Maté, Sabina María Saralegui, Andrés Muñoz-Garay, Carlos Schwarzbaum, Pablo J Herlax, Vanesa
Published in
The Biochemical journal
Alpha hemolysin (HlyA) is the major virulence factor of uropathogenic Escherichia coli (UPEC) strains. Once in circulation, a low concentration of the toxin induces an increase in intracellular calcium that activates calpains - which proteolyse cytoskeleton proteins - and also favours the exposure of phosphatidylserine (PS) in the outer leaflet of ...
Soubeyrand, Eric Kelly, Megan Keene, Shea A Bernert, Ann C Latimer, Scott Johnson, Timothy S Elowsky, Christian Colquhoun, Thomas A Block, Anna K Basset, Gilles J
...
Published in
The Biochemical journal
Plants have evolved the ability to derive the benzenoid moiety of the respiratory cofactor and antioxidant, ubiquinone (coenzyme Q), either from the β-oxidative metabolism of p-coumarate or from the peroxidative cleavage of kaempferol. Here, isotopic feeding assays, gene co-expression analysis and reverse genetics identified Arabidopsis 4-COUMARATE...
Si, Meiru Chen, Can Wei, Zengfan Gong, Zhijin Li, GuiZhi Yao, Shumin
Published in
The Biochemical journal
MarR (multiple antibiotic resistance regulator) proteins are a family of transcriptional regulators that is prevalent in Corynebacterium glutamicum. Understanding the physiological and biochemical function of MarR homologs in C. glutamicum has focused on cysteine oxidation-based redox-sensing and substrate metabolism-involving regulators. In this s...
Legewie, Larissa Loschwitz, Jennifer Steffens, Nora Prescher, Martin Wang, Xue Smits, Sander H J Schmitt, Lutz Strodel, Birgit Degrandi, Daniel Pfeffer, Klaus
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Published in
The Biochemical journal
Guanylate-binding proteins (GBPs) constitute a family of interferon-inducible guanosine triphosphatases (GTPases) that are key players in host defense against intracellular pathogens ranging from protozoa to bacteria and viruses. So far, human GBP1 and GBP5 as well as murine GBP2 (mGBP2) have been biochemically characterized in detail. Here, with m...
Lebredonchel, Elodie Houdou, Marine Hoffmann, Hans-Heinrich Kondratska, Kateryna Krzewinski, Marie-Ange Vicogne, Dorothée Rice, Charles M Klein, André Foulquier, François
Published in
The Biochemical journal
TMEM165 was highlighted in 2012 as the first member of the Uncharacterized Protein Family 0016 (UPF0016) related to human glycosylation diseases. Defects in TMEM165 are associated with strong Golgi glycosylation abnormalities. Our previous work has shown that TMEM165 rapidly degrades with supraphysiological manganese supplementation. In this paper,...
Curtino, Juan A Aon, Miguel A
Published in
The Biochemical journal
Although the discovery of glycogen in the liver, attributed to Claude Bernard, happened more than 160 years ago, the mechanism involved in the initiation of glucose polymerization remained unknown. The discovery of glycogenin at the core of glycogen's structure and the initiation of its glucopolymerization is among one of the most exciting and rela...
Powell, Jason A Pitman, Melissa R Zebol, Julia R Moretti, Paul A B Neubauer, Heidi A Davies, Lorena T Lewis, Alexander C Dagley, Laura F Webb, Andrew I Costabile, Maurizio
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Published in
The Biochemical journal
Sphingosine kinase 1 (SK1) is a signalling enzyme that catalyses the phosphorylation of sphingosine to generate the bioactive lipid sphingosine 1-phosphate (S1P). A number of SK1 inhibitors and chemotherapeutics can induce the degradation of SK1, with the loss of this pro-survival enzyme shown to significantly contribute to the anti-cancer properti...
